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TRUPATH Triple Gaq
Plasmid#196058PurposeEncodes a G alpha subunit (GNAQ) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteinsDepositorUseLuciferaseTagsGFP2 and GSAG linkerExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceFeb. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pDYU1B/mRuby3–α-tubulin
Plasmid#154302PurposeExpresses mRuby3–α-tubulin from actin15 promoter in Dictyostelium discoideumDepositorInsertalpha-tubuin
UseDictyostelium expressionTagsmRuby3ExpressionBacterialMutationPromoterAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCX:cIPAD-3 (full length)
Plasmid#208520PurposeddGFP-A and ddGFP-B3-inserted Pcdhα4DepositorInsertPcdha4 (Pcdha4 Mouse)
UseTagsHA tagExpressionMammalianMutationPromoterChicken β-actinAvailable sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCX:cIPAD-1 (full length)
Plasmid#208519PurposeddGFP-A and ddGFP-B1-inserted Pcdhα4DepositorInsertPcdha4 (Pcdha4 Mouse)
UseTagsHA tagExpressionMammalianMutationPromoterChicken β-actinAvailable sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCX:cIPAD-3 (ΔICD)
Plasmid#208518PurposeddGFP-A and ddGFP-B3-inserted Pcdhα4 lacking an intracellular domain (ICD)DepositorInsertPcdha4 (Pcdha4 Mouse)
UseTagsHA tagExpressionMammalianMutationPromoterChicken β-actinAvailable sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCX:cIPAD-1 (ΔICD)
Plasmid#208517PurposeddGFP-A and ddGFP-B1-inserted Pcdhα4 lacking an intracellular domain (ICD)DepositorInsertPcdha4 (Pcdha4 Mouse)
UseTagsHA tagExpressionMammalianMutationPromoterChicken β-actinAvailable sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEF-XMAS-1xStop
Plasmid#164411PurposeTransient Reporter for Editing Enrichment (TREE) Plasmid. GFP turns on with adenosine base editing (ABE). 1 stop codon.DepositorInsertmCherry
UseCRISPR and Synthetic BiologyTagsT2A-GFPExpressionMammalianMutationPromoterEF1-alphaAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF-XMAS-2xStop
Plasmid#164412PurposeTransient Reporter for Editing Enrichment (TREE) Plasmid. GFP turns on with adenosine base editing (ABE). 2 stop codons.DepositorInsertmCherry
UseCRISPR and Synthetic BiologyTagsT2A-GFPExpressionMammalianMutationPromoterEF1-alphaAvailable sinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV-SFFV-PIK3CA-WPRE-UbC-Emerald
Plasmid#203797PurposeLentiviral vector expressing human phosphoinositide 3-kinase catalytic subunit alpha (PIK3CA) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoterDepositorInsertsUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-CMV-Flag-PGC1a-6His
Plasmid#67637PurposeAAV vector expressing PGC1a geneDepositorInsertPGC1a (Ppargc1a Mouse)
UseAAVTags6XHis and Flag tagExpressionMutationPromoterCMVAvailable sinceApril 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
FH95pUp95GW (B4)
Plasmid#74013Purposelentiviral expression of shRNA resistant Psd95-alpha-EGFP fusion and Psd95 shRNADepositorInsertPsd95 shRNA
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterH1Available sinceMarch 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pKLF10.1.0-gDNA
Plasmid#132433PurposeCRISPR/Cas9 plasmid to create GFP fusion proteinsDepositorInsertKLF10 (KLF10 Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceDec. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pCX:α4-EC4-GA
Plasmid#208514PurposeddGFP-A-inserted Pcdhα4DepositorInsertPcdha4 (Pcdha4 Mouse)
UseTagsHA tagExpressionMammalianMutationPromoterChicken β-actinAvailable sinceOct. 5, 2023AvailabilityAcademic Institutions and Nonprofits only
