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pT7-gRNA_zebrafish_mmp21

Plasmid

#72890

Purpose

plasmid to generate guideRNA against mmp21 gene in zebrafish

Depositor

Nicholas Katsanis

Article

Perles et al J Med Genet. 2015 Dec;52(12):840-7. doi:

Insert

zebrafish mmp21 (mmp21 Zebrafish)

Use

CRISPR

Tags

Expression

Mutation

Promoter

T7 RNA pol

Available since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only

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mKO-Tubulin-6

Plasmid

#57850

Purpose

Localization: Microtubules, Excitation: 548, Emission: 561

Depositor

Michael Davidson

Article

Shroff et al Proc Natl Acad Sci U S A. 2007 Dec 18;10

Insert

Tubulin (TUBA1B Human)

Use

Tags

mKO

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
pBT309a_pBS-U-attPx3-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5

Plasmid

#52542

Purpose

Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attPx3 (70 bp each)
Neo
PhiC31o integrase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pPK1E-2+t-gfp

Plasmid

#48132

Purpose

Shuttle vector E. coli/B.meg.; gfp-expression under control of RNAP-K1E-inducible promoter

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Insert

gfp

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 21, 2013

Availability

Academic Institutions and Nonprofits only

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pCI Flag Smad3 LC AAGY

Plasmid

#27039

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

aa 133-425; P181A P182A

Promoter

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only
pENTR4_FUS_G2A_R521H

Plasmid

#26370

Depositor

Thomas Tuschl

Article

Hoell et al Nat Struct Mol Biol. 2011 Nov 13. doi: 1

Insert

FUS (FUS Human)

Use

Entry vector

Tags

Expression

Mutation

R521H

Promoter

Available since

Sept. 22, 2010

Availability

Academic Institutions and Nonprofits only
pCS2 Flag Smad3 AAGY

Plasmid

#27004

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Expression

Mutation

P181A P182A

Promoter

Available since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only
pCI Flag Smad3 LC WT

Plasmid

#26996

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

Smad3 (SMAD3 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

aa 133-425

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pFastBac1-NpSxph

Plasmid

#193097

Purpose

Protein expression in insect cells

Depositor

Dan Minor

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

Insert

Nanorana parkeri saxiphilin

Use

Tags

3C-eGFP-His10

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac1-NpSxphI559Y

Plasmid

#193099

Purpose

Protein expression in insect cells

Depositor

Dan Minor

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

Insert

Nanorana parkeri saxiphilin I559Y

Use

Tags

3C-eGFP-His10

Expression

Insect

Mutation

I559Y

Promoter

polyhedrin

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac1-OsSxph

Plasmid

#193100

Purpose

Protein expression in insect cells

Depositor

Dan Minor

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

Insert

Oophaga sylvatica saxiphilin

Use

Tags

3C-eGFP-His10

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac1-MaSxph

Plasmid

#193101

Purpose

Protein expression in insect cells

Depositor

Dan Minor

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

Insert

Mantella aurantica saxiphilin

Use

Tags

3C-eGFP-His10

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pFastBac1-RiSxph

Plasmid

#193102

Purpose

Protein expression in insect cells

Depositor

Dan Minor

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

Insert

Ranitomeya imitator saxiphilin

Use

Tags

3C-eGFP-His10

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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RcSxph E540A

Plasmid

#194019

Purpose

Protein expression and characterization

Depositor

Dan Minor

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

Insert

Rana catesbeiana Saxiphilin

Use

Tags

His10 and mEGFP,

Expression

Insect

Mutation

E540A

Promoter

Polyhedrin

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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RcSxph E540D

Plasmid

#194020

Purpose

Protein expression and characterization

Depositor

Dan Minor

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

Insert

Rana catesbeiana Saxiphilin

Use

Tags

His10 and mEGFP,

Expression

Insect

Mutation

E540D

Promoter

Polyhedrin

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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RcSxph E540Q

Plasmid

#194021

Purpose

Protein expression and characterization

Depositor

Dan Minor

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

Insert

Rana catesbeiana Saxiphilin

Use

Tags

His10 and mEGFP,

Expression

Insect

Mutation

E540Q

Promoter

Polyhedrin

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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RcSxph Y558A

Plasmid

#194022

Purpose

Protein expression and characterization

Depositor

Dan Minor

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

Insert

Rana catesbeiana Saxiphilin

Use

Tags

His10 and mEGFP,

Expression

Insect

Mutation

Y558A

Promoter

Polyhedrin

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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RcSxph Y558F

Plasmid

#194023

Purpose

Protein expression and characterization

Depositor

Dan Minor

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

Insert

Rana catesbeiana Saxiphilin

Use

Tags

His10 and mEGFP,

Expression

Insect

Mutation

Y558F

Promoter

Polyhedrin

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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RcSxph Y558I

Plasmid

#194024

Purpose

Protein expression and characterization

Depositor

Dan Minor

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

Insert

Rana catesbeiana Saxiphilin

Use

Tags

His10 and mEGFP,

Expression

Insect

Mutation

Y558I

Promoter

Polyhedrin

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

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RcSxph F561A

Plasmid

#194025

Purpose

Protein expression and characterization

Depositor

Dan Minor

Article

Chen et al Proc Natl Acad Sci U S A. 2022 Nov;119(4

Insert

Rana catesbeiana Saxiphilin

Use

Tags

His10 and mEGFP,

Expression

Insect

Mutation

F561A

Promoter

Polyhedrin

Available since

Sept. 6, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge