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pAV-U6+27-Linear-1_PolyU(4)_Tornado-Corn

Plasmid

#159482

Purpose

Tests for the impact of 4 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-1_PolyU(4)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6-27

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Linear-1_PolyU(5)_Tornado-Corn

Plasmid

#159483

Purpose

Tests for the impact of 5 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-1_PolyU(5)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6-27

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Linear-1_PolyU(6)_Tornado-Corn

Plasmid

#159484

Purpose

Tests for the impact of 6 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-1_PolyU(6)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6-27

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Linear-1_PolyU(7)_Tornado-Corn

Plasmid

#159485

Purpose

Tests for the impact of 7 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-1_PolyU(7)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6-27

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pAV-U6+27-Linear-1_PolyU(8)_Tornado-Corn

Plasmid

#159486

Purpose

Tests for the impact of 8 uracils in a poly-uracil tract on human polymerase III transcription from a U6 promoter.

Depositor

Julius Lucks

Article

Verosloff et al J Mol Biol. 2021 Jun 25;433(13):166978.

Insert

Termination Module:Linear-1_PolyU(8)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6-27

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pDH42(CUG/A)

Plasmid

#154132

Purpose

Expresses engineered (23S-5S phusion) rRNAs operon in bacterial cells

Depositor

Alexander Mankin

Article

Huang et al Nat Commun. 2020 Jun 9;11(1):2900. doi:

Insert

engineered bacterial rRNAs operon under control of pL promoter

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 2, 2020

Availability

Academic Institutions and Nonprofits only

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TB:3xDBE_RE:MiniP

Plasmid

#124529

Purpose

3 Copies of the Daf-16 family binding element

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Insert

3 copies of the Daf-16 family binding element

Use

Luciferase and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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MT2a_K30A

Plasmid

#105694

Purpose

Bacterial expression plasmids for production of recombinant metallothionein 2a K30A mutant

Depositor

Artur Krężel

Article

Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d

Insert

Metallothionein 2a (MT2A Human)

Use

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Mutation

Lysine 30 to Alanine

Promoter

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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MT2a_K31A

Plasmid

#105695

Purpose

Bacterial expression plasmids for production of recombinant metallothionein 2a K31A mutant

Depositor

Artur Krężel

Article

Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d

Insert

Metallothionein 2a (MT2A Human)

Use

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Mutation

Lysine 31 to Alanine

Promoter

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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MT2a_C21A

Plasmid

#105697

Purpose

Bacterial expression plasmids for production of recombinant metallothionein 2a C21A mutant

Depositor

Artur Krężel

Article

Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d

Insert

Metallothionein 2a (MT2A Human)

Use

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Mutation

Cysteine 21 to Alanine

Promoter

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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MT2a_K20A

Plasmid

#105698

Purpose

Bacterial expression plasmids for production of recombinant metallothionein 2a K20A mutant

Depositor

Artur Krężel

Article

Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d

Insert

Metallothionein 2a (MT2A Human)

Use

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Mutation

Lysine 20 to Alanine

Promoter

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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MT2a_S6A

Plasmid

#105699

Purpose

Bacterial expression plasmids for production of recombinant metallothionein 2a S6A mutant

Depositor

Artur Krężel

Article

Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d

Insert

Metallothionein 2a (MT2A Human)

Use

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Mutation

Serine 6 to Alanine

Promoter

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR207-OEP7(1–50)

Plasmid

#100601

Purpose

Gateway entry vector for AtOEP7(1–50)

Depositor

Yutaka Kodama

Article

Tanaka et al J Plant Res. 2017 Jun 20. doi: 10.1007/s

Insert

OEP7(1–50) (OEP7 Mustard Weed)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pET28b-FOXP2_P6

Plasmid

#122649

Purpose

Expresses truncated variant (aa271-aa500) of human FOXP2 in bacterial cells

Depositor

Hendrik Dietz

Article

Haussermann et al Angew Chem Int Ed Engl. 2019 Jun 3;58(23

Insert

Homo sapiens forkhead box P2 (FOXP2 Human)

Use

Tags

His-tag and Strep-tag II

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pET28b-FOXP2_P1

Plasmid

#122645

Purpose

Expresses truncated variant (aa271-aa714) of human FOXP2 in bacterial cells

Depositor

Hendrik Dietz

Article

Haussermann et al Angew Chem Int Ed Engl. 2019 Jun 3;58(23

Insert

Homo sapiens forkhead box P2 (FOXP2 Human)

Use

Tags

His-tag and Strep-tag II

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pET28b-FOXP2_P3

Plasmid

#122647

Purpose

Expresses truncated variant (aa367-aa714) of human FOXP2 in bacterial cells

Depositor

Hendrik Dietz

Article

Haussermann et al Angew Chem Int Ed Engl. 2019 Jun 3;58(23

Insert

Homo sapiens forkhead box P2 (FOXP2 Human)

Use

Tags

His-tag and Strep-tag II

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pET28b-FOXP2_P4

Plasmid

#122648

Purpose

Expresses truncated variant (aa503-aa714) of human FOXP2 in bacterial cells

Depositor

Hendrik Dietz

Article

Haussermann et al Angew Chem Int Ed Engl. 2019 Jun 3;58(23

Insert

Homo sapiens forkhead box P2 (FOXP2 Human)

Use

Tags

His-tag and Strep-tag II

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pNH-TrxT-G-T136D-S15

Plasmid

#127824

Purpose

Production of recombinant human ribosomal protein S15

Depositor

Ivanhoe Leung

Article

Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

Insert

RPS15 (RPS15 Human)

Use

Tags

His6 - Thioredoxin - TEV

Expression

Bacterial

Mutation

T136D

Promoter

T7

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pNH-TrxT-S18

Plasmid

#127826

Purpose

Production of recombinant human ribosomal protein S18

Depositor

Ivanhoe Leung

Article

Correddu et al Sci Rep. 2019 Jun 20;9(1):8884. doi: 10.

Insert

RPS18 (RPS18 Human)

Use

Tags

His6 - Thioredoxin - TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/AUU-nLuc-3XFLAG

Plasmid

#127305

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from AUU start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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