pET28b-FOXP2_P1
(Plasmid #122645)

• Purpose: Expresses truncated variant (aa271-aa714) of human FOXP2 in bacterial cells
• Depositing Lab: Hendrik Dietz
• Publication: Haussermann et al Angew Chem Int Ed Engl. 2019 Jun 3;58(23):7662-7667. doi: 10.1002/anie.201901734. Epub 2019 Apr 29.

Backbone

• Vector backbone: pET28b
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5268
• Total vector size (bp): 6640
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Homo sapiens forkhead box P2
• Alt name: FOXP2
• Species: H. sapiens (human)
• Insert Size (bp): 1338
• GenBank ID: 93986
• Entrez Gene: FOXP2 (a.k.a. CAGH44, SPCH1, TNRC10)
• Promoter: T7
• Tags / Fusion Proteins:
  • His-tag (N terminal on backbone)
  • Strep-tag II (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7 promoter
• 3′ sequencing primer: T7 terminator

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Eurofins Genomics GmbH - Gene synthesis of full-lengths human FOXP2 gene

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry