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Plasmid#136419PurposeLentiviral expression of gRNAs targeting intron 1 of human CTBP2 and intron 1 of human MGMT. Also constitutively expresses Puromycin fused to TagBFP.DepositorInsertU6_sgRNA(CTBP2)_U6_sgRNA(MGMT)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST51-LRRK2-I1371V
Plasmid#29399DepositorInsertLRRK2 (LRRK2 Human)
UseDestination vectorTags6XHis and V5ExpressionMammalianMutationIle1371ValPromoterAvailable sinceApril 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pVP16-tev-PRT1
Plasmid#83405Purposebacterial expression vector pVP16 containing Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studiesDepositorInsertPROTEOLYSIS1 (PRT1) (PRT1 Mustard Weed, E. coli)
UseTagsHis8, MBP, and tev cleavage siteExpressionBacterialMutationPromoterAvailable sinceApril 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
IDG_CLIC2_OE_1
Plasmid#161642PurposeOverexpresses CLIC2DepositorInsertCLIC2 (CLIC2 Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceDec. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1_SARS-COV-2 _NSP1
Plasmid#169376PurposeCell-free/mammalian expression vector for SARS-COV-2 virus NSP1 with N-term eGFPDepositorInsertSARS-COV-2 _NSP1
UseCell-free expression vectorTagsHis and eGFPExpressionMutationCodon optimized for Saccharomyces cerevisiaePromoterT7- CMV/CAGAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDEST51-LRRK2-R1398Q
Plasmid#29398DepositorInsertLRRK2 (LRRK2 Human)
UseDestination vectorTags6XHis and V5ExpressionMammalianMutationArg1398GlnPromoterAvailable sinceApril 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDEST51-LRRK2-T1343G
Plasmid#29400DepositorInsertLRRK2 (LRRK2 Human)
UseDestination vectorTags6XHis and V5ExpressionMammalianMutationThr1343GlyPromoterAvailable sinceApril 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCWX-R4-DEST-R2-PG
Plasmid#45957DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 2, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLV-Enh eGFP Reporter-muKC2-X1
Plasmid#59200PurposeLentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.DepositorInsertkrt1-ex1
UseLentiviralTagsExpressionMutationPromoterMu Hsp68 minimal promoterAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGC157
Plasmid#19682DepositorInsertPrpl-28
UseEntry cloneTagsExpressionWormMutationPromoterAvailable sinceMarch 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBABEpuro-CRAF_S257W
Plasmid#51126Purposemammalian expression of wt CRAF S257WDepositorInsertCRAF (RAF1 Human)
UseRetroviralTagsExpressionMammalianMutationS257WPromoterAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rholike WT
Plasmid#52239PurposeDrosophila expression of YFP-RholikeDepositorInsertRholike (RhoL Fly)
UseDrosophilaTagsYFPExpressionInsectMutationPromoterGAL4Available sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAG426GAL-FES
Plasmid#203501PurposeExpresses human FES kinase from a GAL promoter with a URA3 markerDepositorInsertFES (FES Human)
UseTagsExpressionYeastMutationPromoterGAL1Available sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GALL-SARS-CoV-2_3CL
Plasmid#203496PurposeExpresses SARS-CoV-2 3CL protease from a GALL promoter with a URA3 markerDepositorInsertSARS-CoV-2 3CL protease (ORF1ab )
UseTagsExpressionYeastMutationPromoterGALLAvailable sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL-2x-uA-BWCoV-SW1_3CL
Plasmid#201936PurposeExpresses Beluga Whale-CoV SW1 3CL protease from a GAL-2x promoter with a URA3 markerDepositorInsertBeluga Whale-CoV SW1 3CL protease
UseTagsExpressionYeastMutationPromoterGAL1Available sinceJuly 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAG416GAL10-Bat-CoV-HKU4_3CL
Plasmid#201943PurposeExpresses Bat-CoV HKU4 3CL protease from a GAL10 promoter with a URA3 markerDepositorInsertBat-CoV HKU4 3CL protease (ORF1ab )
UseTagsExpressionYeastMutationPromoterGAL10Available sinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-NXPH4 (neo)
Plasmid#192078PurposeLentivirus for expression of NXPH4DepositorInsertNXPH4 (NXPH4 Human)
UseLentiviralTagsExpressionMutationPromoterhPGKAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCSDest-ENTR-MCP-ADAR2-DD(E488Q/T490A)-V5
Plasmid#192714PurposeMammalian expression vector containing MS2 coat protein fused to ADAR2 catalytic domain (E488Q/T490A) with V5 tagDepositorInsertMCP-ADAR2-DD(E488Q/T490A)-V5
UseTagsV5ExpressionMammalianMutationE488Q/T490APromoterCMVAvailable sinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1_SARS-COV-2_N
Plasmid#169401PurposeCell-free/mammalian expression vector for SARS-COV-2 virus N with N-term eGFPDepositorInsertSARS-COV-2 _N (N SARS-Cov2)
UseCell-free expression vectorTagsHis and eGFPExpressionMutationPromoterT7- CMV/CAGAvailable sinceJuly 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA1_SARS-COV-2_ORF6
Plasmid#169392PurposeCell-free/mammalian expression vector for SARS-COV-2 virus ORF6 with N-term eGFPDepositorInsertSARS-COV-2 _ORF6 (ORF6 SARS-Cov2)
UseCell-free expression vectorTagsHis and eGFPExpressionMutationPromoterT7- CMV/CAGAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only