-
Plasmid#23907DepositorInsertSYK (SYK Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pJYS3_ΔcrtYf
Plasmid#85542PurposeConstitutive transcription of FnCpf1 and crRNA of crtYfDepositorInsertsCpf1
crRNA of crtYf
UseCRISPR; Shuttle vector corynebacterium glutamicum…TagsExpressionBacterialMutationPromoterAvailable sinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
CaMKII-somBiPOLES-mCerulean (AAV5)
Viral Prep#154948-AAV5PurposeReady-to-use AAV5 particles produced from CaMKII-somBiPOLES-mCerulean (#154948). In addition to the viral particles, you will also receive purified CaMKII-somBiPOLES-mCerulean plasmid DNA. CamKII-driven expression of soma-targeted BiPOLES for optogenetic inhibition (blue light) and activation (red light). These AAV preparations are suitable purity for injection into animals.DepositorPromoterCaMKIIa(0.4)TagsmCeruleanAvailable sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FRT-stop-FRT(FSF)- loxP-stop-loxP(LSL)-SP-HA-HRPtm-WPRE-bGHpolyA
Plasmid#197044PurposeIn situ cell-surface proteome extraction by extracellular labeling (iPEEL)DepositorInsertspCAG-FSF-LSL
SP-HA-HRPtm
WPRE-bGHpolyA
UseTagsHA, mycExpressionMammalianMutationPromoterpCAGAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-M2
Plasmid#135583PurposeHigh eukaryotic protein expression shuttle vectorDepositorTypeEmpty backboneUseTransfer vectorTagsExpressionInsectMutationPromoterpolyhedrin of AcMNPVAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
NSK026 CMV-TO-p53R248W (FLP-IN)
Plasmid#191168PurposeExpression of human p53 mutant R248W in mammalian cellsDepositorInsertp53(R248W) (TP53 Human)
UseTagsExpressionMammalianMutationR248WPromoterCMV-TOAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
-
Human MAC-1 alpha
Plasmid#8631DepositorInsertMAC-1 alpha (ITGAM Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-VEGFR2-mCherry
Plasmid#139606PurposeExpresses a VEGFR2-mCherry fusion protein in mammalian cellsDepositorInsertKDR (KDR Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-PM
Plasmid#197386PurposeTo constitutively express Pdx1-Mafa and in cells (Blasticidin resistant).DepositorInsertPDX1-T2A-MAFA
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-f:PGC1
Plasmid#1026DepositorInsertPPAR gamma coactivator 1 alpha (Ppargc1a Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
Kaede-H2B-6
Plasmid#57316PurposeLocalization: Nucleus/Histones, Excitation: 508 / 572, Emission: 518 / 580DepositorInsertH2B (H2BC11 Human)
UseTagsKaedeExpressionMammalianMutationD26G and V119I in H2BPromoterCMVAvailable sinceJan. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFp10
Plasmid#170460PurposeEarlyBac insect cell expression systemDepositorInsertnone
UseTagsExpressionInsectMutationPromoterdHsp70Available sinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDragon-p21
Plasmid#155016PurposeFor Flp-mediated cassette exchange. Expresses tdTomato in the presence of (r)tTA, switching to the cell cycle suppressor p21 in the presence of Cre and (r)tTA activityDepositorInsertCdkn1a (Cdkn1a Mouse)
UseMouse TargetingTagsExpressionMutationPromoterAvailable sinceOct. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFH2.2_mCherry-MS2x12
Plasmid#205521PurposeExpress mCherry-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)DepositorInsertmCherry
UseTagsExpressionMammalianMutationWTPromoterCMVAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-19:LAMP1-mEGFP
Plasmid#101782PurposeHomology arms and linker-mEGFP sequence for C-terminus tagging of human LAMP1DepositorInsertLAMP1 Homology Arms with linker-mEGFP (LAMP1 Human)
UseCRISPR; Donor templateTagslinker-mEGFPExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562)
Plasmid#140002PurposeCMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABEmax(7.10) SpCas9 variant named SpG with P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsP2A-EGFPExpressionMammalianMutationnSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…PromoterCMV and T7Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
hSTARR-seq_ORI vector
Plasmid#99296PurposeVector to measure enhancer activity of candidates from a DNA library by STARR-seq.DepositorTypeEmpty backboneUseHstarr-seq screening vectorTagsExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
vYM112_PB-pEF1a-optiPIN2-HA(Hygro)
Plasmid#160045PurposeFor expressing the active auxin pump PIN2DepositorInsertPIN2
UseSynthetic BiologyTagsHAExpressionMammalianMutationcodon optimizedPromoterpEF1sAvailable sinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-C_NUP214:1-2090
Plasmid#211294PurposeMammalian expression of full-length human NUP214. C-terminal HiBiT tag.DepositorInsertNUP214:M1-S2090 (NUP214 Human)
UseTagsGSSGGSSGVSGWRLFKKISExpressionMammalianMutationP574S natural variant (VAR_045691). S1872N annot…PromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only