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Plasmid#216086PurposeCas9 [Sp] knockout targeting CD47, positive controlDepositorInsertCD47 guide
UseCRISPR and Lentiviral; Positive controlsTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPbHiT_3xmyc_hsp70UTR_hdhfr/yfcu
Plasmid#216421PurposeEmpty backbone to express gene specific gRNA and carry the homology repair template for CRISPR editing of Plasmodium berghei using the PbHiT system.DepositorTypeEmpty backboneUseCRISPR; Expression in plasmodium bergheiTags3xmycExpressionBacterialMutationPromoterAvailable sinceApril 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPbU6_hdhfr/yfcu
Plasmid#216422PurposeEmpty backbone to express gene specific gRNA from the Plasmodium berghei U6 promoter for traditional CRISPR editing.DepositorTypeEmpty backboneUseCRISPR; Expression in plasmodium bergheiTagsExpressionBacterialMutationPromoterAvailable sinceApril 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
hLOXL2-S300P
Plasmid#200061PurposeLOXL2 mutantDepositorInserthuman lysyl oxidase like 2 (LOXL2 Human)
UseTagsExpressionMammalianMutationS300P mutationPromoterAvailable sinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTSAR3.6t
Plasmid#210258PurposeFluorescent reporter for Type III secretion system activity in Shigella flexneriDepositorInsertsmScarlet
superfolder GFP
UseTagsssraExpressionBacterialMutationPromoteripaH7.8p and rpsMAvailable sinceApril 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
GfaABC1D-Crym-eGFP
Plasmid#200080PurposeTo study u-crystallin protein interactors, control plasmidDepositorInsertGfaABC1D-Crym-eGFP
UseAAV and Mouse TargetingTagseGFPExpressionMutationPromoterGfaABC1DAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
Human USP47
Plasmid#217624PurposeMammalian expression of USP47DepositorInsertUSP47 (USP47 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
Human V5-USP47
Plasmid#217626PurposeMammalian expression of V5 tagged USP47DepositorInsertUSP47 (USP47 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pColdI-GST-mGTSF2
Plasmid#215714PurposeBacterial expression of GST-tagged GTSF2DepositorInsertGTSF2 (Gtsf2 Mouse)
UseTags6x His and GSTExpressionBacterialMutationPromoterCspAAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
B2375_CRY2olig-mEGFP
Plasmid#215109PurposeExpresses CRY2olig (E490G CRY2 1-498) fused to monomeric EGFP in mammalian cellsDepositorInsertCRY2olig-mEGFP (CRY2 Mustard Weed)
UseTagsmEGFPExpressionMammalianMutationE490G, 1-498PromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
B2094_CRY2olig-miRFP
Plasmid#215114PurposeExpresses CRY2olig (E490G CRY2 1-498) fused to miRFP in mammalian cellsDepositorInsertCRY2olig-miRFP (CRY2 Mustard Weed)
UseTagsmiRFPExpressionMammalianMutationE490GPromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2 RPS28 UTR1 SCR
Plasmid#203158PurposeRPS28 UTR luciferase assay plasmid (SCR UTR1, sites 1 and 2)DepositorInsertRPS28 UTR (RPS28 Human)
UseLuciferaseTagsExpressionMutationscrambled the nucleotides in the two MIR-28 bindi…PromoterAvailable sinceMarch 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTSAR3.7t
Plasmid#210259PurposeFluorescent reporter for Type III secretion system activity in Shigella flexneriDepositorInsertse2Crimson
superfolder GFP
UseTagsssraExpressionBacterialMutationPromoteripaH7.8p and rpsMAvailable sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTSAR3.3t
Plasmid#210255PurposeFluorescent reporter for Type III secretion system activity in Shigella flexneriDepositorInsertsDsRed
superfolder GFP
UseTagsssraExpressionBacterialMutationPromoteripaH7.8p and rpsMAvailable sinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-P1K-3VSV-Atg8-MET15
Plasmid#207055PurposeExpression of 3VSV-Atg8. Uses auxotrophic marker MET15(Saccharomyces cerevisiae).DepositorInsertATG8
UseTags3xVSVExpressionYeastMutationPromoterpATG8Available sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Zeo-BFP-P2A-otPKR-ML
Plasmid#214365PurposeExpression of the Blue Fluorescent Protein - P2A - medium length protein kinase R from chinook salmonDepositorInsertBFP-P2A-otPKRML
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Zeo-BFP-P2A-otPKR-SL
Plasmid#214366PurposeExpression of the Blue Fluorescent Protein - P2A - short length protein kinase R from chinook salmonDepositorInsertBFP-P2A-otPKRSL
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Zeo-BFP-P2A-otPKR-FL
Plasmid#214364PurposeExpression of the Blue Fluorescent Protein - P2A - full length protein kinase R from chinook salmonDepositorInsertBFP-P2A-otPKRFL
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
P1K-3HA-Atg8∆R-MET15
Plasmid#207034PurposeExpression of 3HA-Atg8∆R. This variant bypasses the need of Atg4 for initial C-terminal processing.DepositorInsertATG8
UseTags3xHAExpressionYeastMutation∆RPromoterpATG8Available sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pXU01
Plasmid#212151PurposePlasmid expressing xylitol dehydrogenase under inducible promoter PT7 control. Plasmid confers the ability to use xylitol as major carbon source to E. coli.DepositorInsertxylitol dehydrogenase
UseTags6x HisExpressionBacterialMutationPromoterPT7Available sinceJan. 30, 2024AvailabilityAcademic Institutions and Nonprofits only