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Plasmid#192670PurposeLentiviral expression of sgRNA targeting hASCL1 promoter to activate human ASCL1 transcriptionDepositorInsertHuman ASCL1 activating gRNA #1 (ASCL1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
LentiU6-hIL1RN gRNA_1-MS2-Puro
Plasmid#192677PurposeLentiviral expression of sgRNA targeting hIL1RN promoter to activate human IL1RN transcriptionDepositorInsertHuman IL1RN activating gRNA #1 (IL1RN Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
KTK_139
Plasmid#180565PurposeSpacer sequence in D1.1 format. Allows for Level 2 cloning with D1.2DepositorInsertSpacer sequence in D1.1 format. Allows for Level 2 cloning with D1.2
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
mTln1-F2F3-P229L-pET151
Plasmid#177879PurposeExpresses mouse Talin 1 F2F3-P229L domains in bacterial cellsDepositorInsertmouse Talin 1 F2F3 P229L mutant (Tln1 Mouse)
UseTagsHis-tag, TEV cleavage siteExpressionBacterialMutationchanged Proline 229 to Leucine (P229L)PromoterT7Available sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
mTln1-F2F3-P229S-pET151
Plasmid#177878PurposeExpresses mouse Talin 1 F2F3-P229S domains in bacterial cellsDepositorInsertmouse Talin 1 F2F3 P229S mutant (Tln1 Mouse)
UseTagsHis-tag, TEV cleavage siteExpressionBacterialMutationchanged Proline 229 to Serine (P229S)PromoterT7Available sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
KTK_113
Plasmid#180540PurposeEntry vector containing Signal peptide region of CsgA including SecB-N22 sequencedDepositorInsertSignal peptide region of CsgA including SecB-N22 sequenced
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-R3-ccdB/CmR-R4-bpFOG-B5::B1-NLSLacZ-B2
Plasmid#186414PurposeAttR3/R4 destination vector for ascidian electroporation with AttB1/B2 recombined NLSLacZ under control of AttB4/B5-recombined pFOG ectodermal regulatory sequence.DepositorInsertlacZ (lacZ E. coli)
UseGateway destination vectorTagsNuclear localization signalExpressionMutationPromoterBasal promoter of the FOG geneAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
PHR-GFP-STAT2
Plasmid#183931PurposeOptogenetic PHR domain coupled to GFP and STAT2 activation domain; binds to CIBN upon blue light exposureDepositorInsertPHR-GFP-STAT2 (CRY2 Synthetic, Mustard Weed)
UseTagsExpressionMammalianMutationnonePromoterCMVAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
PHR-GFP-Rta
Plasmid#183930PurposeOptogenetic PHR domain coupled to GFP and Rta activation domain; binds to CIBN upon blue light exposureDepositorInsertPHR-GFP-Rta (CRY2 Synthetic, Mustard Weed)
UseTagsExpressionMammalianMutationnonePromoterCMVAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
PHR-GFP-VPR
Plasmid#183928PurposeOptogenetic PHR domain coupled to GFP and VPR activation domain; binds to CIBN upon blue light exposureDepositorInsertPHR-GFP-VPR (CRY2 Synthetic, Mustard Weed)
UseTagsExpressionMammalianMutationnonePromoterCMVAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
PHR-GFP-FUSN
Plasmid#183932PurposeOptogenetic PHR domain coupled to GFP and FUSN domain with a high phase separation propensity; binds to CIBN upon blue light exposureDepositorInsertPHR-GFP-FUSN (CRY2 Synthetic, Mustard Weed)
UseTagsExpressionMammalianMutationnonePromoterCMVAvailable sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
KTK_095
Plasmid#180524PurposedCas9 KTK compatible plasmid. Contains dCas9 and lacZ dropout region with flanking D1.1 overhangs for insertion of gRNA expression assemblyDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
KTK_324
Plasmid#180566PurposeSpacer sequence in 2.2b format to allow for level 3 cloning with D2.2aDepositorInsertSpacer sequence in 2.2b format to allow for level 3 cloning with D2.2a
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delZn1-L713F
Plasmid#173937PurposeBacterial expression of PARP1 lacking Zn1 domain but containing L713F gain-of-function mutationDepositorInsertPARP1delZn1-L713F (PARP1 Human)
UseTags6xHisExpressionBacterialMutationDeletion of residues 1-96; L713F, V762APromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1delZn3-L713F
Plasmid#173934PurposeBacterial expression of PARP1 lacking Zn3 domain but containing L713F gain-of-function mutationDepositorInsertPARP1delZn3-L713F (PARP1 Human)
UseTags6xHisExpressionBacterialMutationDeletion of residues 214-373; L713F, V762APromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-CAT-L713F
Plasmid#173944PurposeBacterial expression of isolated PARP1 CAT domain containing L713F gain-of-function mutationDepositorInsertPARP1-CAT-L713F (PARP1 Human)
UseTags6xHisExpressionBacterialMutationL713F, V762APromoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-G871L
Plasmid#174798PurposeBacterial expression of a hyperactive PARP1 mutant (G871L destabilizes the autoinhibitory HD subdomain)DepositorInsertPARP1-G871L (PARP1 Human)
UseTags6xHisExpressionBacterialMutationG871L, V762APromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-A870L
Plasmid#174797PurposeBacterial expression of a hyperactive PARP1 mutant (A870L destabilizes the autoinhibitory HD subdomain)DepositorInsertPARP1-A870L (PARP1 Human)
UseTags6xHisExpressionBacterialMutationA870L, V762APromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-K893I
Plasmid#174799PurposeBacterial expression of an inactive mutant (K893I may disrupt NAD+ binding)DepositorInsertPARP1-K893I (PARP1 Human)
UseTags6xHisExpressionBacterialMutationK893I, V762APromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET41sIII_GST-ALC1-K77R
Plasmid#174813PurposeBacterial expression of inactive ALC1 (K77R disrupts ATP binding)DepositorInsertALC1-K77R (CHD1L Human)
UseTagsGSTExpressionBacterialMutationK77RPromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only