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Plasmid#71357Purposecsd6, construct:13-330, pET28b(+) N-tag, mutation:H155ADepositorInsertHP0518 (HP0518 changed Histidine 155 to Alanine)
UseTagsHistidine tagExpressionBacterialMutationPromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
csd6_13-330_Y132A/pET28b(+) N-tag
Plasmid#71355Purposecsd6, construct:13-330, pET28b(+) N-tag, mutation:Y132ADepositorInsertHP0518 (HP0518 changed Tyrosine 132 to Alanine)
UseTagsHistidine tagExpressionBacterialMutationPromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-F-Med26-NTD R61A K62A
Plasmid#49256PurposeMammalian expression of flag-tagged human Med26-NTD R61A K62ADepositorInsertMed 26 (MED26 Human)
UseTagsFlagExpressionMammalianMutationNTD R61A K62APromoterAvailable sinceFeb. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-F-Med26-NTD- K74A K75A
Plasmid#49252PurposeMammalian expression of flag-tagged human Med26-NTD- K74A K75ADepositorInsertMed 26 (MED26 Human)
UseTagsFlagExpressionMammalianMutationK74A, K75A, aa 1-339PromoterAvailable sinceDec. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Tag4A-MMP8 (E87D)
Plasmid#29547DepositorInsertMetalloproteinase protein 8 (MMP8 Human)
UseTagsFLAGExpressionBacterial and MammalianMutationE87DPromoterAvailable sinceSept. 16, 2011AvailabilityAcademic Institutions and Nonprofits only -
PCMV-Tag4A-MMP8 (E138Q)
Plasmid#29549DepositorInsertMetalloproteinase protein 8 (MMP8 Human)
UseTagsFLAGExpressionBacterial and MammalianMutationE138QPromoterAvailable sinceJuly 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
CS-TRE-BclXL-Ubc-rtTA
Plasmid#198189PurposeGeneration of Expandable megakaryocyte cell lines from human iPS cellsDepositorInserthBCLXL (BCL2L1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBactin-DIO-inv[roxSTOProx-EB3-mNeonGreen-bGH]
Plasmid#196887PurposeDual Cre/Dre-dependent expression of EB3-mNeon. Used as functional (labelling of microtubule plus ends) Cre-Dre cotrasfection reporterDepositorInsertroxSTOProx-EB3 mNeonGreen-bGH (MAPRE3 Human)
UseCre/Lox; Dre/roxTagsExpressionMammalianMutationPromoterChicken bactin (plus Chicken bactin intron)Available sinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
R620-M11-303: CMV51p> SARS-CoV-2 S(1-1213)-2P-T4f-His6
Plasmid#170195PurposeMammalian expression of SARS-CoV-2 soluble spike trimer proteinDepositorInsertSARS-CoV-2 S (spike) (S SARS-CoV-2)
UseTagsHis6ExpressionMammalianMutationPromoterCMV51Available sinceNov. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLN484 (FuGW-S(cMYC)p-[GAD-Ex1]-[miR1-Mv3 intron]-[GAD-Ex2]-11Pe)
Plasmid#105192PurposeModule 1 - synthetic promoter cMYC drives self-inhibiting GAD expression (see PMID: 29056342 for detailed information)DepositorInsertS(cMYC)p-[GAD-Ex1]-[miR1-Mv3 intron]-[GAD-Ex2]-11Pe
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLN552 (FuGW-S(USF1)p-[GAD-Ex1]-[miR1-Mv3 intron]-[GAD-Ex2]-11Pe)
Plasmid#105202PurposeModule 1 - synthetic promoter USF1 drives self-inhibiting GAD expression (see PMID: 29056342 for detailed information)DepositorInsertS(USF1)p-[GAD-Ex1]-[miR1-Mv3 intron]-[GAD-Ex2]-11Pe
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCashGem-Xyl2-URA3-LINEAR
Plasmid#174840PurposepRS414 backbone containing LINEAR fragment targeting XYL2 in S. stipitisDepositorInsertCodon optimized Cas9:hGem
UseCRISPR and Synthetic BiologyTagshGeminin tagExpressionBacterial and YeastMutationPromoterENO1pAvailable sinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCB’
Plasmid#162703PurposepCB reconstructed after selection for CCMB1 growth on glycerol under ambient airDepositorInsertpHnCB10 derived carboxysome operon, prk
UseTagsExpressionBacterialMutation5513T>G (tetR E37A); 7758G>T (second tet op…PromoterPLtet0-1 promoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET151-GyrA_F164R238
Plasmid#216230PurposeS. aureus USA300 GyrA mutant F164 R238 residues codon-optimized for E. coliDepositorInsertDNA gyrase
UseTags6xHisExpressionBacterialMutationChanged arginine 238 to serine, phenylalanine 168…PromoterT7Available sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET151-GyrB_A467
Plasmid#216234PurposeS. aureus USA300 GyrB mutant A467 residue codon-optimized for E. coliDepositorInsertDNA gyrase
UseTags6xHisExpressionBacterialMutationChanged alanine 467 to valinePromoterT7Available sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET151-GyrB_T522
Plasmid#216235PurposeS. aureus USA300 GyrB mutant T522 residue codon-optimized for E. coliDepositorInsertDNA gyrase
UseTags6xHisExpressionBacterialMutationChanged threonine 522 to isoleucinePromoterT7Available sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Puro_hPGK1_hGEM(1-110)_mNG_hSLBP(18-108)_FS
Plasmid#191527PurposeDonor for targeted integration of 3xFLAG-2xSTREP tagged S-phase specific probe (FUCCI-S) to the AAVS1 safe harbor locusDepositorInserthGEM(1-110)_mNG_hSLBP(18-108)
UseCRISPR and Synthetic BiologyTagsmNeonGreen (mNG) + 3xFLAG_TwinStrepExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
spoT_NS1
Plasmid#100914Purposeexpresses spoT (from native promoter) from the NS1 site in the S. elongatus PCC7942 genomeDepositorInsertsynpcc7942_1377 (spoT)
UseCyanobacteria cloning vectorTagsExpressionMutationPromoterAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
spoT-
Plasmid#100913Purposedeletes synpcc7942_1377 (spoT)DepositorInsertKanamycin casette (in place of spoT gene) flanked by S. elongatus sequence
UseCloning vectorTagsExpressionMutationPromoterAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
HL 716
Bacterial Strain#60368PurposeLim Lab StrainDepositorBacterial ResistanceNoneAvailable sinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only