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Plasmid#223251PurposeMammalian expression of C-terminally Flag-tagged optimized R2Tg proteinDepositorInsertR2Tg protein_opt
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
TagRFP-T_A1aY1
Plasmid#158751PurposeLentiviral vector for expression of TagRFP-T_A1aY1 in mammalian cells for detection of tyrosinated microtubules.DepositorInsertTagRFP-T_A1aY1
UseLentiviralTagsTagRFP-TExpressionMammalianMutationPromoterCAG (CMV enhancer and chicken beta actin promoter)Available sinceOct. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5FRT/TO_HA-mAID-nanobody
Plasmid#117713PurposeExpression of tetracycline-inducible HA-tagged lysine-less mAID fused to lysine-less vhhGFP4 (mAID-nanobody)DepositorInsertmAID-nanobody
UseTagstriple HAExpressionMammalianMutationcodon optimized mAID for murine expressionPromoterCMVAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHCMV_FLUAV_HA
Plasmid#207273PurposeMammalian expression of Influenza A virus HA proteinDepositorInsertFLUAV_HA (HA Influenza A virus)
UseTagsExpressionMammalianMutationmammalian codon optimizationPromoterCMV promoterAvailable sinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2260
Plasmid#141998PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSOX17 (SOX17 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pWSK29
Plasmid#172972PurposeLow copy number cloning vectorDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFUW-tetO-GATA2
Plasmid#125028Purposedoxycycline-inducible expression of human GATA2 in mammalian cellsDepositorInsertGATA binding protein 2 (GATA2 Human)
UseLentiviralTagsNoneExpressionMammalianMutationPromoterTRE-mCMVAvailable sinceJune 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
hCRISPRi-v2, Kinases, Phosphatases, and Drug Targets (h1), top 5 sgRNAs/gene
Pooled Library#83971PurposeHuman CRISPRi Pooled Library tartgeting kinsases, phosphatases and Drug TargetsDepositorAvailable sinceDec. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTransposon.CAG.CasRX-P2A-Blast
Plasmid#212965PurposeEpisomal plasmid encoding CasRx-P2A under the control of the CAG promoter. The construct is flanked by PB and SB repeats for integration into the cell genome.DepositorInsertCasRx
UseTransposonTagsP2A-Blasticidin resistanceExpressionMammalianMutationPromoterCAGGSAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpyTag-sfGFP
Plasmid#184226PurposeExpresses SpyTag-superfolder GFP in the bacterial cytoplasm. SpyTag enables covalent reaction with SpyCatcher.DepositorInsertSpyTag-sfGFP
UseTagsHis6, SpyTag, and TEV protease cleavage siteExpressionBacterialMutationPromoterT5Available sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHCMV-SS-antiFLAGscFv-TM
Plasmid#198303PurposeDisplays anti-FLAG scFv from the surface of mammalian cellsDepositorInsertpHCMV-SS-antiFLAGscFv-TM
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
CS-TRE-BclXL-Ubc-rtTA
Plasmid#198189PurposeGeneration of Expandable megakaryocyte cell lines from human iPS cellsDepositorInserthBCLXL (BCL2L1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pB80-hKif5b(1-560)rigor-2xmNeongreen
Plasmid#174649PurposeLive-cell marker for stable microtubulesDepositorInserthKif5B(1-560)rigor-2xmNeongreen (KIF5B Human)
UseTagsmNeongreen-mNeongreenExpressionMammalianMutationrigor mutation G234APromoterChicken beta-actinAvailable sinceMarch 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPGC-K
Plasmid#174581PurposepOPIN-GG expression vector compatible with C-terminal tagging, Kanamycin resistantDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
ACBE-Q
Plasmid#204608PurposeMammalian expression of high-accuracy adenine-to-cytosine base editor ACBE-QDepositorInsertbpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e (N108Q)-linker-C terminal of nSpCas9-bpNLS-P2A-GFP
UseCRISPRTags6*HisExpressionMammalianMutationTadA-8e (N108Q); mAAG (R165E/Y179F); S. pyogenes …PromoterAvailable sinceAug. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pYFP-RPB1aAmr
Plasmid#75284PurposeExpresses alpha-amanitin resistant EYFP tagged POLR2A in mammalian cellsDepositorInsertPOLR2A (POLR2A Human)
UseTagsEYFPExpressionMammalianMutationN792D alpha-amanitin–resistantPromoterCMVAvailable sinceJuly 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDF-Frb-v1
Plasmid#201923PurposeE. coli vector expressing FrbA, FrbB, FrbC, FrbD and FrbE for the biosynthesis of non-hydrolyzable phosphoserine from phosphoenolpyruvate.DepositorInsertsFrbA
FrbB
FrbC
FrbD
FrbE
UseTagsExpressionBacterialMutationPromoterT7 (modified), T7 (modified, moderate), T7 (modif…Available sinceJune 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lenti-entry-puro
Plasmid#85745PurposeLentiviral vector for generating multiple sgRNAs carrying plasmids by Golden Gate Assembly.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT3TS-spRYCas9-ABE-UMax
Plasmid#222139PurposeNear PAMless Adenine base editor with modified TadA variant for A-to-G base editing in zebrafishDepositorInsertTadA-nSpRYCas9
UseCRISPR; Zebrafish expressionTagsbipartite NLSExpressionMutationPromoterT3Available sinceJuly 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-29b(+)_His-CBD-SpEndoH
Plasmid#218196PurposeBacterial expression of a His-tagged Chitin Binding fusion of Streptomyces plicatus EndoHDepositorInsertEndoH
UseTagstufA-His-CBDExpressionBacterialMutationcodon optimized Ecoli, residues 45-313PromoterT7Available sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only