Addgene

We narrowed to 1,095 results for: piggyBac

Showing: 781 - 800 of 1095 results

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pAS_4xhyb PylT A41AA C55A LaG17-SynNotch 204TAA 442TAG

Plasmid

#154778

Purpose

plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and LaG17-SynNotch 204TAA 277TAG, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

LaG17-SynNotch

Use

Tags

LaG17

Expression

Mammalian

Mutation

204TAA 442TAG in LaG17-SynNotch, hybrid PylT with…

Promoter

EF1

Availability

Academic Institutions and Nonprofits only

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pAS_4xhyb PylT A41AA C55A LaG17-SynNotch 204TAG 442TAA

Plasmid

#154779

Purpose

plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and LaG17-SynNotch 204TAG 277TAA, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

LaG17-SynNotch

Use

Tags

LaG17

Expression

Mammalian

Mutation

204TAG 442TAA in LaG17-SynNotch, hybrid PylT with…

Promoter

EF1

Availability

Academic Institutions and Nonprofits only

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pPB-MPP8_Flag2-Blast

Plasmid

#175038

Purpose

Mammalian expression of full length mMPP8 with C-terminal Flag2 tag

Depositor

Kristian Helin

Article

Muller et al Nat Commun. 2021 May 24;12(1):3034. doi:

Insert

Mphosph8 (Mphosph8 Mouse)

Use

Piggybac

Tags

Flag2

Expression

Mammalian

Mutation

Promoter

CAG

Availability

Academic Institutions and Nonprofits only

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PB-REVERBA-mScarlet-NLS-PEST

Plasmid

#190658

Purpose

Fluorescent reporter for circadian rhythms

Depositor

Mary Teruel

Article

Zhang et al Proc Natl Acad Sci U S A. 2022 Aug 16;11

Insert

Reverba-mScarlet (Nr1d1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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EK0379 CMV-TO-EGFP-stop-t70587 (PB)

Plasmid

#191154

Purpose

Expression of Trigger 1 in the 3' UTR of EGFP in mammalian cells; used to make a cell line to study sensors for integrated 3' UTR or CDS triggers; expression is inducible with doxycycline due to CMV-TO promoter.

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

EGFP-t70587

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

CMV-TO

Availability

Academic Institutions and Nonprofits only

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NSK025 CMV-TO-EGFP:c.637A>G(N213D; CCA>CCG)-stop-t70587 (PB)

Plasmid

#191166

Purpose

Mammalian expression of a EGFP mutant N213D derived from EK0379.

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

EGFP(N213D)

Use

Tags

Expression

Mammalian

Mutation

c.637A>G(N213D; CCA>CCG)

Promoter

CMV-TO

Availability

Academic Institutions and Nonprofits only

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PB-Hyg-Dest-mCherry-PAR-6B

Plasmid

#194908

Purpose

To generate mCherry-PAR-6B (mouse) expressing stable cell lines with piggybac transposase co-expression and hygromycin selections.

Depositor

Clare Buckley

Article

Liang et al EMBO J. 2022 Aug 22:e111021. doi: 10.152

Insert

PAR-6B (Pard6b Mouse)

Use

Mouse Targeting

Tags

mCherry

Expression

Mammalian

Mutation

39 C to G, silent mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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PB-TO-hNIL BSD-mApple

Plasmid

#182313

Purpose

Piggybac Tet-ON plasmid for differentiating hiPSCs into lower motor neurons via NGN2, ISL1, and LHX3 expression, bsd selection, mApple

Depositor

iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

Inserts

hNGN2 (NEUROG2 Human)
hISL1 (ISL1 Human)
hLHX3 (LHX3 Human)
bsd-T2A-mycNLS-mApple

Use

Tags

T2A-mycNLS-mApple

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pB[nos-Cas9 PUb-ZsGreen]

Plasmid

#169011

Purpose

For germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.

Depositor

Max Scott

Article

Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

Insert

Cas9

Use

Piggybac

Tags

Expression

Insect

Mutation

Promoter

Drosophila melanogaster nanos

Availability

Academic Institutions and Nonprofits only

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pB[vasa-Cas9 PUb-ZsGreen]

Plasmid

#169012

Purpose

For germline transformation. Expresses Cas9 in germ cells and ZsGreen marker in all cells.

Depositor

Max Scott

Article

Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

Insert

Cas9

Use

Piggybac

Tags

Expression

Insect

Mutation

Promoter

Drosophila melanogaster vasa

Availability

Academic Institutions and Nonprofits only

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pAC150-RFP-EGFP-LGALS3 R186S

Plasmid

#175779

Purpose

PiggyBac Vector expressing GFP-RFP-LGALS3 R186S

Depositor

Wade Harper

Article

Eapen et al Elife. 2021 Sep 29;10. pii: 72328. doi:

Insert

LGALS3 (LGALS3 Human)

Use

Tags

Expression

Mammalian

Mutation

R186S

Promoter

Availability

Academic Institutions and Nonprofits only

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ePB-Bsd-CAG-LoxP-dsRed_PA-LoxP-SV40-NLS-NG-T2A-hSHH

Plasmid

#179586

Purpose

ePiggyBac vector that has a blasticidin selectable cassette and a LoxP exchangeable dual colors cassette with SHH expression (Red module-dsRed/Green module-NeonGreen T2A SHH)

Depositor

Ali Hemmati Brivanlou

Article

De Santis et al Nat Commun. 2021 Nov 19;12(1):6768. doi:

Insert

NLS-NG-T2A-hSHH

Use

Piggybac

Tags

NeonGreen

Expression

Mutation

Promoter

TRE

Availability

Academic Institutions and Nonprofits only

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pAC150-KEIMA-REEP5

Plasmid

#201928

Purpose

PiggyBac Vector expressing KEIMA-REEP5

Depositor

Wade Harper

Article

Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

Insert

REEP5 (REEP5 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pAC150-KEIMA-RAMP4

Plasmid

#201929

Purpose

PiggyBac Vector expressing KEIMA-RAMP4

Depositor

Wade Harper

Article

Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

Insert

RAMP4 (SERP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pAC150-TEX264ΔLIR-GFP

Plasmid

#201930

Purpose

PiggyBac Vector expressing TEX264ΔLIR-GFP

Depositor

Wade Harper

Article

Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

Insert

TEX264 (TEX264 Human)

Use

Tags

Expression

Mammalian

Mutation

F273A

Promoter

Availability

Academic Institutions and Nonprofits only

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pAC150-TEX264-GFP

Plasmid

#201931

Purpose

PiggyBac Vector expressing TEX264-GFP

Depositor

Wade Harper

Article

Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

Insert

TEX264 (TEX264 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pAC150-FAM134C-GFP

Plasmid

#201932

Purpose

PiggyBac Vector expressing FAM134C-GFP

Depositor

Wade Harper

Article

Hoyer et al Nat Cell Biol. 2024 Mar;26(3):378-392. d

Insert

FAM134C (RETREG3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pKH151_PB_flag-LC3B_wt_IRES-BFP

Plasmid

#214976

Purpose

Flag tagged LC3B

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

FLAG-LC3B (MAP1LC3B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pKH152_PB_flag-LC3B_LDSmut_IRES-BFP

Plasmid

#214977

Purpose

Flag tagged LDS mutant LC3B

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

FLAG-LC3B_mut (MAP1LC3B Human)

Use

Tags

Expression

Mammalian

Mutation

LDS mutant

Promoter

Availability

Academic Institutions and Nonprofits only

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PB-TRE-dCas9-Zim3-MeCP2

Plasmid

#220143

Purpose

doxycycline-inducible dCas9-Zim3-MeCP2 expression for Epigenetic repression

Depositor

Frank Buchholz

Article

Ding et al Life Sci Alliance. 2022 Mar 14;5(6). pii

Insert

dCas9-Zim3-MeCP2

Use

CRISPR and Synthetic Biology; Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

Tre-3G

Availability

Academic Institutions and Nonprofits only

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