Addgene

We narrowed to 13,814 results for: pET

Showing: 781 - 800 of 13814 results

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pET11a+CYP119

Plasmid

#66131

Purpose

Expresses the wild type of CYP119

Depositor

Teruyuki Nagamune

Article

Suzuki et al Biotechnol J. 2014 Dec;9(12):1573-81. do

Insert

CYP119 (cyp119 )

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

June 29, 2015

Availability

Academic Institutions and Nonprofits only

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pET28b::mAID

Plasmid

#15923

Depositor

Nina Papavasiliou

Article

Besmer et al Mol Cell Biol. 2006 Jun . 26(11):4378-85

Insert

AID (Aicda Mouse)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only

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pET28a-ec_bPAC

Plasmid

#28135

Purpose

Expression of photoactivated cyclase bPAC of Beggiatoa sp. optimized for E. coli

Depositor

Peter Hegemann

Article

Stierl et al J Biol Chem. 2011 Jan 14. 286(2):1181-8.

Insert

E.coli adapted photoactivated adenylyl cyclase

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

March 31, 2011

Availability

Academic Institutions and Nonprofits only

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pET11a-STEPtag

Plasmid

#159373

Purpose

Expresses STEPtag in E. coli

Depositor

Roberto Chica

Article

Eason et al ACS Synth Biol. 2020 Nov 20;9(11):2955-2

Insert

STEPtag

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pETSUMO-TtAgocatdead

Plasmid

#210322

Purpose

Bacterial expression of the His- and SUMO-tagged catalytically inactive Argonaute protein of Thermus thermophilus

Depositor

Phillip Zamore

Article

Jolly et al Cell. 2020 Sep 17;182(6):1545-1559.e18.

Insert

TtAgo (TT_P0026 Thermus thermophilus)

Use

Tags

6x His tag and SUMO tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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SIRT5H158Y-pET15b

Plasmid

#81032

Purpose

Bacterial expression of Human catalytic mutant SIRT5 protein

Depositor

David Lombard

Article

Kumar et al Methods Mol Biol. 2016;1436:241-57. doi:

Insert

Sirt5 (SIRT5 Human)

Use

Tags

His

Expression

Bacterial

Mutation

H158Y catalytic mutant

Promoter

T7

Available since

Aug. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pET29b-EPN-01*

Plasmid

#80423

Purpose

For expression of EPN-01* in E. coli

Depositor

Wesley Sundquist

Article

Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

Insert

EPN-01*

Use

Tags

Myc

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only
pET15TV-L-AldA WT

Plasmid

#187976

Purpose

Expresses human aldolase A

Depositor

Audrey Lamb

Article

Fenton et al Protein Sci. 2022 Feb;31(2):357-370. doi

Insert

AldA WT (ALDOA Human)

Use

Tags

Hexahistag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pET15b-pA-Mnase

Plasmid

#124883

Purpose

To transform BL21-Gold(DE3) and induced by IPTG for His-proteinA-MNase fusion protein.

Depositor

Keji Zhao

Article

Ku et al Genome Res. 2021 Apr 14. pii: gr.260893.

Insert

protein A-MNase

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-Rx3(A7)

Plasmid

#35525

Depositor

Robert Cohen

Article

Sims et al Nat Methods. 2012 Feb 5. doi: 10.1038/nm

Insert

His6-Rx3(A7)

Use

Tags

His6

Expression

Bacterial

Mutation

Changed G2 to P

Promoter

T7

Available since

April 5, 2012

Availability

Academic Institutions and Nonprofits only

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pET32a-HD16Q

Plasmid

#11487

Depositor

Pamela Bjorkman

Article

Bennett et al Proc Natl Acad Sci U S A. 2002 Sep 3. 99

Insert

huntingtin, exon 1, 16 glutamines (HTT Human)

Use

Tags

His, His (out of frame), and Trx

Expression

Bacterial

Mutation

huntingtin exon 1 with 16 glutamines, vector m…

Promoter

Available since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
pETCON-TNF

Plasmid

#67748

Purpose

Tumor Necrosis Factor Alpha Yeast Display Plasmid

Depositor

Timothy Whitehead

Article

Kowalsky et al J Biol Chem. 2015 Oct 30;290(44):26457-7

Insert

TNF-Alpha

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pET20b-delta133HtrA2

Plasmid

#14126

Depositor

L. Miguel Martins

Article

Martins et al J Biol Chem. 2003 Dec 5. 278(49):49417-2

Insert

HtrA2 (HTRA2 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

March 7, 2007

Availability

Academic Institutions and Nonprofits only

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pET28-MRPP1

Plasmid

#67862

Purpose

bacterial expression of TRMT10C, full coding sequence (mitoch. form) + N-term. His tag (thrombin site)

Depositor

Walter Rossmanith

Article

Holzmann et al Cell. 2008 Oct 31;135(3):462-74. doi: 10

Insert

MRPP1 (TRMT10C Human)

Use

Tags

His

Expression

Bacterial

Mutation

mitoch. form (contains aa 40-403)

Promoter

T7

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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YPet-H2B-6

Plasmid

#56632

Purpose

Localization: Histone H2B, Excitation: 513, Emission: 527

Depositor

Michael Davidson

Insert

H2B (H2BC11 Human)

Use

Tags

YPet

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available since

Dec. 19, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pET28a-cMSPdH45

Plasmid

#199767

Purpose

Expresses split-intein fused MSPdH45 for circularization during expression (internal His-Tag)

Depositor

Franz Hagn

Article

Miehling et al Chembiochem. 2018 Sep 17;19(18):1927-193

Insert

cMSPdH45

Use

Tags

His6-Tag

Expression

Bacterial

Mutation

internal His-Tag

Promoter

Available since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pET3a-Ub-D77

Plasmid

#18896

Depositor

Cecile Pickart

Article

Raasi et al Methods Mol Biol. 2005 . 301():47-55.

Insert

Ubiquitin-D77 (UBC Human)

Use

Tags

Expression

Bacterial

Mutation

Aspartate residue is added to the C-terminus of t…

Promoter

Available since

Sept. 10, 2008

Availability

Academic Institutions and Nonprofits only
pET32a-hHBEGF

Plasmid

#199234

Purpose

Bacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.

Depositor

Nilson Zanchin

Article

Ferreira et al Sci Rep. 2022 Mar 23;12(1):5034. doi: 10

Insert

Proheparin-binding EGF-like growth factor (HBEGF Human)

Use

Tags

TrxA-6xHis-S-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pET-3c-rhVTN-N

Plasmid

#30225

Depositor

James Thomson

Article

Chen et al Nat Methods. 2011 Apr 10. ():.

Insert

rhVitronectin with N terminus cleavage (VTN Human)

Use

Tags

Expression

Bacterial

Mutation

deleted amino acids: 20*-62

Promoter

Available since

May 25, 2011

Availability

Academic Institutions and Nonprofits only

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pET28a-cMSP1E3D1

Plasmid

#199772

Purpose

Expresses split-intein fused MSP1E3D1 for circularization during expression (external His-Tag)

Depositor

Franz Hagn

Article

Daniilidis et al J Mol Biol. 2022 Dec 30;434(24):167861.

Insert

cMSP1E3D1

Use

Tags

His6-Tag

Expression

Bacterial

Mutation

Extended cMSP1D1; external His-Tags flanking the …

Promoter

Available since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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