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Plasmid#70185PurposeConstiutive mammalian expression of the bioluminescent GpNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects..DepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only
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pRetroX-Tight-MCS_PGK-OgNLuc
Plasmid#70186PurposeConstiutive mammalian expression of the bioluminescent OgNLuc LumiFluor reporter for single-cell analysis and sensitive optical imaging of living subjects.DepositorTypeEmpty backboneUseRetroviralTagsExpressionMammalianMutationPromoterPGKAvailable sinceDec. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
CAG sHRPb-FKBP-post mGRASP
Plasmid#73146PurposeSmall fragment of split HRP and FKBP fused to the extracellular terminus of the "post mGRASP" scaffold published by Magee, in which the majority of the NLG1 extracellular domain is deleted.DepositorInsertsHRPb-FKBP-post mGRASP
UseTagsHA tagExpressionMammalianMutationPromoterCAGAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
AAV-GFAP(long)-FLEX-GCaMP7f
Plasmid#189612PurposeCre-dependent GCaMP7f under the GFAP promoterDepositorInsertGCaMP7f
UseAAV and Cre/LoxTagsGCaMP7fExpressionMammalianMutationPromoterAvailable sinceSept. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSP2-48-merTetO-EFS-BLaR
Plasmid#118712PurposeContains an optimized 48-mer TetO repeat for imaging of desired lociDepositorInsertsBlasticidin resistance gene
EFS-NS promoter
UseTagsExpressionMammalianMutationPromoterEFS-NSAvailable sinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXL-BacII-CAG-loxP-STOP-loxP-Venus
Plasmid#170520PurposePlasmid containing piggyBac ITRs flanking CAG, a loxP-STOP-loxP cassette and Venus (also see doi: 10.7554/eLife.63907 )DepositorInsertCAG-loxP-STOP-loxP-Venus
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
rAAV-syn::FLEX-rev::PSAMQ79G,Q139G:5HT3HC-IRES-GFP
Plasmid#32475DepositorInsertPSAMQ79G,Q139G-5HT3HC
UseAAV and Cre/Lox; Adeno-associated virus, flex swi…TagsIRES-EGFPExpressionMutationPromoterSynapsinAvailable sinceDec. 12, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBH-UAS-secA5-YFP
Plasmid#32359PurposeLive imaging of apoptotic cells in zebrafishDepositorInsertsecreted human annexin 5 (ANXA5 Human)
UseZebrafish expressionTagsExpressionMutationPromoterUASAvailable sinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
AAV-FLIM-AKAR
Plasmid#63058PurposeExpresses the Fluorescence Lifetime Imaging Microscopy (FLIM)-compatible PKA activity sensor FLIM-AKAR in an AAV backboneDepositorInsertFLIM-AKAR
UseAAVTagsExpressionMammalianMutationPromoterEF-1αAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAdSh.U6.gRNAGFP
Plasmid#58255PurposeU6 promoter-driven single guide RNA complementary to eGFPDepositorInsertU6.gRNAGFP cassette
UseAdenoviral and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceSept. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMCS.71/2272.Hygro
Plasmid#22731DepositorInsertLox71-FRT-PGK-hygro-FRT-Lox2272
UseCre/LoxTagsExpressionMutationThis is a basal exchange vector for RMCE. The vec…PromoterAvailable sinceDec. 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
Cyan Nano-lantern/pcDNA3
Plasmid#67660Purposecyan luminescent protein for high-speed single-cell and whole body imagingDepositorInsertCyan Nano-lantern
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
Orange Nano-lantern/pcDNA3
Plasmid#67661Purposeorange luminescent protein for high-speed single-cell and whole body imagingDepositorInsertOrange Nano-lantern
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)_mtZFN(-)2G
Plasmid#62799PurposemtZFN2G architecture bearing (-) FokI domainDepositorInsertmtZFN(-)2G
UseTagsInternal FLAG tag immediately after F1B mitochond…ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)_mtZFN(+)2G
Plasmid#62798PurposemtZFN2G architecture bearing (+) FokI domainDepositorInsertmtZFN(+)2G
UseTagsInternal HA tag immediately after F1B mitochondri…ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMCS.71/2272
Plasmid#22732DepositorInsertLox71-MCS-Lox2272
UseCre/LoxTagsExpressionMutationThis is a basal exchange vector for RMCE containi…PromoterAvailable sinceDec. 17, 2009AvailabilityAcademic Institutions and Nonprofits only -
Nano-lantern-plasma membrane/pcDNA3
Plasmid#51975Purposemammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, targeted to the plasma membraneDepositorInsertNano-lantern
UseTagsHIS and LynExpressionMammalianMutationS257G in Rluc8PromoterAvailable sinceApril 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAdSh.U6.gRNAS1
Plasmid#58252PurposeU6 promoter-driven single guide RNA complementary to the human AAVS1 locusDepositorInsertU6.gRNAS1 cassette
UseAdenoviral and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceSept. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pPpT4- HTA1prom-TIR1 F74G -FLAG
Plasmid#189728PurposeEnables expression of OsTIR1(F74G) from the HTA1 promoter, used to implement the AID2 system in Komagataella phaffii (original name pLB220)DepositorInsertOsTIR1
UseTagsFLAGExpressionYeastMutationPromoterHTA1Available sinceSept. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
Orange Nano-lantern/pRSETB
Plasmid#67659Purposeorange luminescent protein for high-speed single-cell and whole body imagingDepositorInsertOrange Nano-lantern
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only