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Addgene
Showing: 781 - 800 of 1204 results

  3. pcDNA3.1(+)-ER-BSCaM2

    Plasmid
    #64855
    Purpose
    Endoplasmic reticulum (ER)-targeted version of a high-affinity FRET-based biosensor for monitoring Ca2+/CaM levels; targeted to cytosolic face of ER membrane.
    Depositor
    Jin Zhang
    Article
    Mehta et al Elife. 2014 Jul 23;3:e03765. doi: 10.755
    Insert
    CYP450-BSCaM2
    Use
    Tags
    CFP, N-terminal 27 amino acids from CYP450, and Y…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    64855-plasmid-map-sequence-id-109681

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  16. pMesh1*

    Plasmid
    #175591
    Purpose
    Lac inducible, AMP Resistant, fluor labelled (CFP) gene mesh1 from D. melanogaster on a low copy backbone. Used to induce controllable hydrolysis of ppGpp in E. coli.
    Depositor
    Greg Bokinsky
    Article
    Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.
    Insert
    Mesh1
    Use
    Tags
    Fused with a flexible GS linker to cerulean
    Expression
    Bacterial
    Mutation
    Codon optimized for translation in E.coli. AGGAGG…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    175591-plasmid-map-sequence-id-348365

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  17. MSP1830

    Plasmid
    #70708
    Purpose
    Human expression plasmid for SaCas9 KKH variant: CAG-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Insert
    mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Mammalian
    Mutation
    E782K, N968K and R1015H in SaCas9
    Promoter
    CAG
    Availability
    Academic Institutions and Nonprofits only
    70708-plasmid-map-sequence-id-364684

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Showing: 781 - 800 of 1204 results