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Bacterial Strain#37985DepositorBacterial ResistanceKanamycinAvailable sinceJuly 5, 2012AvailabilityAcademic Institutions and Nonprofits only
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GDAP2-c005
Plasmid#173110PurposeExpression of recombinant fusion protein in E. coliDepositorInsertGDAP2 (GDAP2 Human)
UseTagsmCeruleanExpressionBacterialMutationM1-N231PromoterAvailable sinceNov. 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSQT834
Plasmid#53371PurposeCsy4 and Cas9 nuclease expression plasmidDepositorInsertCsy4-T2A-Cas9-NLS
UseCRISPRTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSQT1601
Plasmid#53369Purposehuman codon optimized Csy4 and FokI-dCas9 expression plasmidDepositorInserthCsy4-T2A-NLS-hFokI-dCas9-NLS
UseCRISPRTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-RhoA FLARE.sc Biosensor WT
Plasmid#12150DepositorInsertRhoA Biosensor (RHOA Human)
UseTags6xHisExpressionBacterial, Insect, and Mammā¦MutationPromoterAvailable sinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pBabe-Puro-RhoA FLARE.sc Biosensor
Plasmid#12602DepositorInsertRhoA Biosensor (RHOA Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
RNF146-c005
Plasmid#173091PurposeExpression of recombinant fusion protein in E. coliDepositorInsertRNF146 (RNF146 Human)
UseTagsmCeruleanExpressionBacterialMutationR99-L183, codon optimizedPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.1(+)-ER-BSCaM2
Plasmid#64855PurposeEndoplasmic reticulum (ER)-targeted version of a high-affinity FRET-based biosensor for monitoring Ca2+/CaM levels; targeted to cytosolic face of ER membrane.DepositorInsertCYP450-BSCaM2
UseTagsCFP, N-terminal 27 amino acids from CYP450, and Yā¦ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-SNAP-HCA_sensor
Plasmid#127182PurposeEukaryotic expression of the fusion protein used for sulfa-drugs sensingDepositorInsertSNAP-YPeT-Pro15-GGS2-Pro15-CFP-HCA
UseTagsHisTag and StrepTagExpressionMammalianMutationPromoterCMV-TetO2Available sinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
CoV-NSP3-c001
Plasmid#173082PurposeExpression of recombinant fusion protein in E. coliDepositorInsertrep
UseTagsmCeruleanExpressionBacterialMutationE1000-K1173, codon optimizedPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGen2.1-redCALWY-4
Plasmid#50738PurposeExpresses redCALWY-4 in mammalian cells. FRET sensor for zinc using mOrange2-R125I and mCherry-R125I as donor and acceptor domains. Allows multiparameter imaging together with CFP-YFP FRET sensors.DepositorInsertgene for redCALWY-4 Zn2+ sensor
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGen2.1-redCALWY-1
Plasmid#50739PurposeExpresses redCALWY-1 in mammalian cells. FRET sensor for zinc using mOrange2-R125I and mCherry-R125I as donor and acceptor domains. Allows multiparameter imaging together with CFP-YFP FRET sensors.DepositorInsertgene for redCALWY-1 Zn2+ sensor
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEBTet-p52-HDM2_sensor
Plasmid#127184PurposeEukaryotic expression of the fusion protein used for drug diruption the p53-HDM2 interactionDepositorInsertp53-YPeT-Pro15-GGS2-Pro15-CFP-HDM2
UseTagsHisTag and StrepTagExpressionMammalianMutationPromoterCMV-TetO2Available sinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)-SNAP-HCA_sensor
Plasmid#127181PurposeBacterial expression of the fusion protein used for sulfa-drugs sensingDepositorInsertSNAP-YPeT-Pro15-GGS2-Pro15-CFP-HCA
UseTagsHisTag and StrepTagExpressionBacterialMutationPromoterT7Available sinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Nuc-deltaCKAR
Plasmid#222430PurposeFRET-based reporter for monitoring deltaPKC activity at the nucleus in cells.DepositorInsertNucleus delta-selective C kinase activity reporter
UseTagsCFP and YFPExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Golgi-deltaCKAR
Plasmid#222428PurposeFRET-based reporter for monitoring deltaPKC activity at the Golgi in cells.DepositorInsertGolgi delta-selective C kinase activity reporter
UseTagsCFP and YFPExpressionMammalianMutationPromoterAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLBT0180
Plasmid#172814PurposeLevel 1 module containing TP-CFP_P4 FDepositorInsertp35S-5UTMV-CTPSSU-CFP-3U+TER35S
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only