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Showing: 61 - 80 of 422 results

  2. pLenti-nSpCas9-APOBEC1

    Plasmid
    #209039
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where APOBEC1 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    APOBEC1-nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209039-plasmid-map-sequence-id-413619

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  3. pLenti-nSpCas9-APOBEC3A

    Plasmid
    #209040
    Purpose
    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where APOBEC3A is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS
    Depositor
    Thomas Gonatopoulos-Pournatzis
    Article
    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e
    Insert
    APOBEC3A-nSpCas9
    Use
    CRISPR and Lentiviral
    Tags
    FLAG-HA
    Expression
    Mammalian
    Mutation
    D10A
    Promoter
    Tight TRE promoter
    Available since
    June 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    209040-plasmid-map-sequence-id-413592

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  12. pEGFP mCherry-ApoB-EGFP

    Plasmid
    #112859
    Purpose
    Plasmid bearing the editing target of APOBEC1 fused between the mCherry cds and the EGFP one. In presence of APOBEC1, the CAA codon in ApoB is edited to a Stop codon (UAA), leading to loss of EGFP
    Depositor
    Silvestro Conticello
    Article
    Severi et al RNA Biol. 2015;12(4):389-97. doi: 10.108
    Insert
    mCherry-apoB-EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    Oct. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112859-plasmid-map-sequence-id-224731

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  15. pEGFP ratAPOBEC1 L173A/G227A

    Plasmid
    #167169
    Purpose
    Expresses rat APOBEC1 L173A/G227A in mammalian cells. Please note that this does not express GFP.
    Depositor
    Silvestro Conticello
    Article
    Chieca et al bioRxiv 2021
    Insert
    APOBEC1 (Apobec1 Rat)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    changed Leucine 173 to Alanine; changed Glycine 2…
    Promoter
    CMV promoter
    Available since
    May 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    167169-plasmid-map-sequence-id-335363

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  16. pEGFP-N1-mCherry-ApoB-TagBFP

    Plasmid
    #129383
    Purpose
    Plasmid bearing the editing target of APOBEC1 fused between the mCherry and the Tag-BFP one. In presence of APOBEC1, the CAA codon in ApoB is edited to a Stop codon (UAA), leading to loss of Tag-BFP
    Depositor
    Silvestro Conticello
    Article
    Chieca et al Methods Mol Biol. 2021;2181:69-81. doi:
    Insert
    mCherry-ApoB-Tag-BFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV promoter
    Available since
    May 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    129383-plasmid-map-sequence-id-254328

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  20. p2T-CMV-EA-evoAPOBEC1max_NG-BlastR

    Plasmid
    #232721
    Purpose
    C-to-T base editor
    Depositor
    David Liu
    Insert
    evoAPOBEC1max H47E+S48A-nCas9(NG)-UGI-UGI
    Use
    Tags
    Expression
    Mammalian
    Mutation
    within evoAPOBEC1: H47E+S48A; within Cas9 D10A
    Promoter
    Available since
    March 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    232721-plasmid-map-sequence-id-463541

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Showing: 61 - 80 of 422 results