-
Plasmid#189938PurposeExpresses CRISPRai cassette under EF1a promoterDepositorInsertCRISPRai cassette
UseTagsExpressionMammalianMutationPromoterCMV enhancer-rat EF1 alphaAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRyl_AXP
Plasmid#84608PurposeIntroduces DSB at AXP locus in Y. lipolyticaDepositorInsertCas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterUAS1B8-TEF(136)Available sinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pL-CRISPR.SFFV.PAC
Plasmid#57829PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA, Puromycin resistance, SFFV Promoter drivenDepositorInsertsSpCas9
Sp sgRNA scaffold
SFFV
P2A-PAC
UseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
p15a_Kan_MLS_Rosa26_Short_CRISPRDel_FNeoStop_PStop_WPRE_bgh_pA
Plasmid#97012PurposeRosa26 targeting vector for intersectional Cre and FLP responsive cassetteDepositorTypeEmpty backboneUseCRISPR and Cre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR_eGFP_497
Plasmid#111824PurposeKnockout eGFPDepositorInsertgRNA targeting eGFP 477-497
UseCRISPRTagsmCherryExpressionBacterial and MammalianMutationPromoterU6Available sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
p15a_Kan_MLS_Rosa26_Short_CRISPRDel
Plasmid#97008PurposeRosa26 locus targeting vectorDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2_sgCtrl#2
Plasmid#174149PurposeLentiviral vector expressing Cas9 and a control sgRNA that targets a safe harbor siteDepositorInsertControl sgRNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRv2_RB1#2
Plasmid#174151PurposeLentiviral vector expressing Cas9 and a sgRNA against the human RB1 geneDepositorInsertRB1 (RB1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRi-D2
Plasmid#182927Purposeinducible CRISPRi plasmid with gRNA targeting to psbD gene in Synechocystis 6803DepositorInsertddcpf1
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR_eGFP_191
Plasmid#111821PurposeKnockout eGFPDepositorInsertgRNA targeting eGFP 171-191
UseCRISPRTagsmCherryExpressionBacterial and MammalianMutationPromoterU6Available sinceAug. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lenti_CRISPRi_sgNeg1
Plasmid#166998PurposeLentiviral expression of negative control sgRNA for CRISPRi knockdownDepositorInsertNegative control sgRNA 1
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPRCreb5gRNA
Plasmid#195021PurposeSequence specific sgRNA that guide Cas9 to the genomic region encoding the bovine Creb5 DNA binding domainDepositorInsertCreb5 gRNA (CREB5 Bovine)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRyl_MFE1
Plasmid#84612PurposeIntroduces DSB at MFE1 locus in Y. lipolyticaDepositorInsertCas9
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterUAS1B8-TEF(136)Available sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJ2072.2_CRISPRlator
Plasmid#140674PurposeCRISPRlatorDepositorInsertCRISPRlator
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
CRISPR_TRAC
Plasmid#164993PurposeExpression of gRNA targeting TCRalpha constant locusDepositorInsertgRNA against TRAC
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR_DMS3g24
Plasmid#89244PurposePlasmid encodes a synthetic CRISPR containing 7 copies of a single spacer, which targets a region (gene 24) in the genome of bacteriophage DMS3DepositorInsertCRISPR
UseTagsExpressionMutationPromoterAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
CRISPR_TRBC
Plasmid#164994PurposeExpression of gRNA targeting TCRbeta constant locusDepositorInsertgRNA against TRBC
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lenti_CRISPRi_sgAR2
Plasmid#167001PurposeLentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdownDepositorInsertAR Promoter sgRNA 2 (AR Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
Lenti_CRISPRi_sgAR1
Plasmid#167000PurposeLentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdownDepositorInsertAR Promoter sgRNA 1 (AR Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceApril 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv1_SDHA
Plasmid#177980Purposelentiviral vector expressing Cas9 and a sgRNA targeting SDHADepositorInsertsgRNA targeting SDHA (SDHA Human)
UseLentiviralTagsExpressionMammalianMutationN/APromoterU6Available sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only