Addgene

We narrowed to 7,178 results for: Trac;

Showing: 61 - 80 of 7178 results

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pUC-tracrRNA-PMtU6-26

Plasmid

#209191

Purpose

Cloning of sgRNAs for expression in plants

Depositor

Christian Staehelin

Insert

MtU6-26 SnRNA promoter

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pUC-tracrRNA-PMtU6-1

Plasmid

#209190

Purpose

Cloning of sgRNAs for expression in plants

Depositor

Christian Staehelin

Insert

MtU6-1 SnRNA promoter

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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ClonTracer Barcoding Library (2.5 ug)

Pooled Library

#67267

Purpose

High complexity 30bp-long semi random DNA barcode library in lentiviral backbone

Depositor

Frank Stegmeier

Article

Bhang et al Nat Med. 2015 Apr 13. doi: 10.1038/nm.38

Expression

Mammalian

Species

Synthetic

Use

Lentiviral

Available since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only
ClonTracer Barcoding Library (7.5 ug)

Pooled Library

#69830

Purpose

High complexity 30bp-long semi random DNA barcode library in lentiviral backbone

Depositor

Frank Stegmeier

Article

Bhang et al Nat Med. 2015 Apr 13. doi: 10.1038/nm.38

Expression

Mammalian

Species

Synthetic

Use

Lentiviral

Available since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only
pWN_U6-TRAC-miniGag-Cas9

Plasmid

#228959

Purpose

miniEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter)

Depositor

Jennifer Doudna

Article

Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

Insert

miniGag-Cas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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Notch3 intracellular domain-pcw107-V5

Plasmid

#64623

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

NOTCH3 (NOTCH3 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

intracellular domain *

Promoter

PGK

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
Notch1 intracellular domain-pcw107-V5

Plasmid

#64622

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

NOTCH1 (NOTCH1 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

intracellular domain *

Promoter

PGK

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only
pAd-Track Flag-SIRT1

Plasmid

#8438

Depositor

Pere Puigserver

Article

Rodgers et al Nature 2005 Mar 3;434(7029):113-8.

Insert

SIRT1 (Sirt1 Mouse)

Use

Adenoviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 4, 2007

Availability

Academic Institutions and Nonprofits only

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tracrRNA(scr)-CJ8421_04975 mRNA

Plasmid

#199718

Purpose

Expresses the Campylobacter mRNA CJ8421_04975 and tracrRNA with scrambled anti-repeat sequencesequence

Depositor

Chase Beisel

Article

Jiao et al Nat Biotechnol. 2023 Jan 5. doi: 10.1038

Insert

CJ8421_04975 mRNA and scrambled tracrRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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Notch1 intracellular domain-pcw107

Plasmid

#64621

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

NOTCH1 (NOTCH1 Human)

Use

Lentiviral

Tags

Expression

Mutation

intracellular domain *

Promoter

PGK

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
LRP4 (extracellular) scFv [N207/27]

Plasmid

#190537

Purpose

Mammalian Expression of LRP4 (extracellular) scFV. Derived from hybridoma N207/27.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

LRP4 (extracellular) (Mus musculus) recombinant scFV (Lrp4 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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1199 pAdTrack FKHR AAA

Plasmid

#9037

Depositor

William Sellers

Article

Ramaswamy et al Cancer Cell. 2002 Jul . 2(1):81-91.

Insert

FKHR AAA (FOXO1 Human)

Use

Adenoviral

Tags

flag

Expression

Mammalian

Mutation

T24A S256A S319A

Promoter

Available since

Sept. 28, 2005

Availability

Academic Institutions and Nonprofits only
pAd-Track HA PGC-1 alpha

Plasmid

#14427

Depositor

Pere Puigserver

Article

Lerin et al Cell Metab. 2006 Jun . 3(6):429-38.

Insert

PGC-1 alpha (Ppargc1a Mouse)

Use

Adenoviral

Tags

HA

Expression

Mammalian

Mutation

Last four amino acids of PGC-1a removed to make t…

Promoter

Available since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only
1200 pAdTrack FKHR H215R

Plasmid

#9038

Depositor

William Sellers

Article

Ramaswamy et al Cancer Cell. 2002 Jul . 2(1):81-91.

Insert

FKHR H215R (FOXO1 Human)

Use

Adenoviral

Tags

flag

Expression

Mammalian

Mutation

H215R

Promoter

Available since

Sept. 28, 2005

Availability

Academic Institutions and Nonprofits only
pAd-Track SIRT1 H355A

Plasmid

#8441

Depositor

Pere Puigserver

Article

Rodgers et al Nature 2005 Mar 3;434(7029):113-8.

Insert

SIRT1 (Sirt1 Mouse)

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

Histidine 355 mutated to Alanine

Promoter

Available since

Dec. 4, 2007

Availability

Academic Institutions and Nonprofits only
lentiGuide-Puro-Chen-tracrRNA_2xBsmBI

Plasmid

#196710

Purpose

Backbone for cloning single guides (Chen tracrRNA PMID: 24360272) or guide libraries with untranscribed barcodes and a tracrRNA of choice

Depositor

Bernhard Schmierer

Article

Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pAd-Track Control siRNA

Plasmid

#8437

Depositor

Pere Puigserver

Article

Rodgers et al Nature 2005 Mar 3;434(7029):113-8.

Type

Empty backbone

Use

Adenoviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 4, 2007

Availability

Academic Institutions and Nonprofits only
pEJS2194-RP_PiggyBac_SpCas9ABE8e-tracrRNA-Puro

Plasmid

#211821

Purpose

PiggyBac vector expressing SpCas9-ABE8e with tracrRNA for stable cell line establishment

Depositor

Erik Sontheimer

Article

Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

Insert

SpCas9-ABE8e, tracrRNA and Puromycin resistance gene

Use

Tags

Expression

Mammalian

Mutation

Promoter

chicken β-actin promoter, U6 promoter and hPGK pr…

Available since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS-HZ08-pAAVsc.U6-tracrRNA

Plasmid

#211816

Purpose

AAV vector expressing tracrRNA under U6 promoter

Depositor

Erik Sontheimer

Article

Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

Insert

U6-tracrRNA

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-ADAM22 (extracellular) [N57/2R]

Plasmid

#177564

Purpose

Mammalian Expression Plasmid of anti-ADAM22 (extracellular) (Mouse). Derived from hybridoma N57/2.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-ADAM22 (extracellular) (Mus musculus) recombinant mouse monoclonal antibody (Adam22 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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