-
Plasmid#127919PurposeTransient expression of biotin ligase BirA and p19 in plant protoplastDepositorInsertBirA
UseTagsExpressionPlantMutationPromoter35SAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1_V5_BirA*_FLAG_Nt
Plasmid#194070Purpose3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blastiDepositorTypeEmpty backboneUseLentiviralTagsV5-BirA(R118G)-FLAGExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1_V5_BirA*_FLAG_Ct
Plasmid#194071Purpose3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-blastiDepositorTypeEmpty backboneUseLentiviralTagsFLAG-BirA(R118G)-V5ExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
BirA*-HA-NES
Plasmid#214473PurposeLentiviral vector for expression in mammalian cells of the proximity ligase BirA(R118G) with a C-terminal HA tag followed by a nuclear exclusion sequence.DepositorInsertBirA(R118G)
UseLentiviral; GatewayTagsHA tag and NES (Nuclear Exclusion Sequence)ExpressionMammalianMutationR118GPromoterEF-1aAvailable sinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
Kusabira Orange-N1 (KO)
Plasmid#54793PurposeLocalization: N1 Cloning Vector, Excitation: 548, Emission: 561DepositorTypeEmpty backboneUseTagsKusabira OrangeExpressionMammalianMutationPromoterAvailable sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
LLP791_PB_dCas9-SSSavi_BirA
Plasmid#211786PurposePiggyBac plasmid with dCas9 docking array with four tag domains (Spy, Snoop, aGCN4, Avi), and BirA, with Hygro selectionDepositorInsertdCas9, Spy, Snoop, aGCN4, AviTags
UseTags3xHAExpressionMammalianMutationdCas9 D10A and H840APromoterpCAGG abd pEF1aAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pR I-Ab alpha BirA
Plasmid#113638PurposeInsect cell expression vector for I-Ab alpha chain fused to BirA biotinylation siteDepositorInsertI-Ab alpha BirA (H2-Ab1 Mouse)
UseTagsBirA biotinylation signal site and fused to Fos-J…ExpressionInsectMutationtransmembrane and cytoplasmic domains truncatedPromoterMetallothioneinAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
rgef-1p::NmBirA
Plasmid#79971Purposeencodes E.coli biotin-ligase under neuronal promotor for C.elegansDepositorInsertBirA
UseTagsNLS::mycExpressionWormMutationPromoterrgefAvailable sinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
BirA_BVES untagged
Plasmid#196581Purposeexpresses BirADepositorInsertBirA_Ecoli
UseBaculovirus expressionTagsExpressionMutationPromoterAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
Kusabira Orange-C1 (KO)
Plasmid#54792PurposeLocalization: C1 Cloning Vector, Excitation: 548, Emission: 561DepositorTypeEmpty backboneUseTagsKusabira OrangeExpressionMammalianMutationPromoterAvailable sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
mKusabira Orange-N1
Plasmid#55046PurposeEmpty backbone for fusing gene of interest to N-term of mKODepositorTypeEmpty backboneUseTagsmKOExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5_FRB-pBirA-HA
Plasmid#83415Purposeproximity-directed biotin labeling on the FRB domain of mTOR in mammalian cellDepositorInsertFRB-pBirA-HA (MTOR Synthetic, Human)
UseTagsHA and pBirAExpressionMammalianMutationPromoterCMV-FAvailable sinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
U_(+36GFP)-BirA
Plasmid#71753PurposeU+36GFP-BirA for expression in E.ColiDepositorInsertU_(pos36GFP)_BirA_6XHis_LPETG
UseTags6XHisExpressionBacterialMutationPromoterAvailable sinceDec. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
Lyn11-BirA*-HA
Plasmid#214474PurposeLentiviral vector for expression in mammalian cells of the proximity ligase BirA(R118G) with an N-terminal Lyn11 plasma membrane localization sequence and C-terminal HA tag.DepositorInsertBirA(R118G)
UseLentiviral; GatewayTagsHA tag and Lyn11 (Plasma Membrane Localization Se…ExpressionMammalianMutationR118GPromoterEF-1aAvailable sinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
MCAM-BirA*-HA
Plasmid#214472PurposeLentiviral vector for the expression of MCAM-BirA*-HA fusion protein in mammalian cells.DepositorInsertMCAM (MCAM Human)
UseLentiviral; GatewayTagsBirA(R118G) and HA tagExpressionMammalianMutationPromoterEF-1aAvailable sinceMarch 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-BirA-ER
Plasmid#20856DepositorInsertBirA-ER
UseTagsExpressionMammalianMutationC-terminal ER targeting sequence (KDEL)PromoterAvailable sinceMay 7, 2009AvailabilityAcademic Institutions and Nonprofits only -
secretedBirA-8his
Plasmid#32408DepositorInsertBirA
UseTags8 His tagExpressionMammalianMutationPromoterAvailable sinceJan. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBirA-P19
Plasmid#127919PurposeTransient expression of biotin ligase BirA and p19 in plant protoplastDepositorInsertBirA
UseTagsExpressionPlantMutationPromoter35SAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
Kusabira Orange-N1 (KO)
Plasmid#54793PurposeLocalization: N1 Cloning Vector, Excitation: 548, Emission: 561DepositorTypeEmpty backboneUseTagsKusabira OrangeExpressionMammalianMutationPromoterAvailable sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pR I-Ab alpha BirA
Plasmid#113638PurposeInsect cell expression vector for I-Ab alpha chain fused to BirA biotinylation siteDepositorInsertI-Ab alpha BirA (H2-Ab1 Mouse)
UseTagsBirA biotinylation signal site and fused to Fos-J…ExpressionInsectMutationtransmembrane and cytoplasmic domains truncatedPromoterMetallothioneinAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only