1. p426 72Q GPD

   Plasmid

   #1183

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 72Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

These results may also be relevant.

1. Flag-Braf R188L

   Plasmid

   #118338

   Purpose

   Expression of human Braf

   Depositor

   Philip Stork

   Article

   Takahashi et al Mol Cell Biol. 2017 Jul 10. pii: MCB.001

   Insert

   Braf R188L (BRAF Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   R188L

   Promoter

   CMV

   Available since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. hSUMO1-HIS

   Plasmid

   #118361

   Purpose

   This plasmid expresses the full length human SUMO1.

   Depositor

   Lea Sistonen

   Article

   Hietakangas et al Mol Cell Biol. 2003 Apr . 23(8):2953-68.

   Insert

   hSUMO1 (SUMO1 Human)
   

   Use

   Tags

   HIS

   Expression

   Mammalian

   Mutation

   Please see Depositor Comments

   Promoter

   Available since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. ScSpe1_pET20b(+)

   Plasmid

   #118343

   Purpose

   Expresses the His tagged Ornithine Decarboxylase from Saccharomyces cerevisiae in Escherichia coli BL21 or similar strains

   Depositor

   Markus Ralser

   Insert

   SPE1
   

   Use

   Tags

   Histidine

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Flag-Craf R401H

   Plasmid

   #118334

   Purpose

   Expression of human Craf

   Depositor

   Philip Stork

   Article

   Takahashi et al Mol Cell Biol. 2017 Jul 10. pii: MCB.001

   Insert

   Craf R401H (RAF1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   R401H

   Promoter

   CMV

   Available since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. GFP-Rap1b S179AS180A

   Plasmid

   #118327

   Purpose

   Expression of bovine Rap1b

   Depositor

   Philip Stork

   Article

   Takahashi et al J Biol Chem. 2013 Sep 27;288(39):27712-2

   Insert

   Rap1b S179AS180A (RAP1B Bovine)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   S179AS180A

   Promoter

   Available since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. hMEF2A K403R

   Plasmid

   #118355

   Purpose

   This plasmid expresses human MEF2A K403R mutant. This construct cannot undergo sumoylation on K403.

   Depositor

   Lea Sistonen

   Article

   Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

   Insert

   hMEF2A (MEF2A Human)
   

   Use

   Tags

   myc and 6xHIS

   Expression

   Mammalian

   Mutation

   K403R

   Promoter

   Available since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Flag-Craf SSDD

   Plasmid

   #118337

   Purpose

   Expression of human Craf

   Depositor

   Philip Stork

   Article

   Takahashi et al Mol Cell Biol. 2017 Jul 10. pii: MCB.001

   Insert

   Craf SSDD (RAF1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   SSDD

   Promoter

   CMV

   Available since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Flag-Fos S374A

   Plasmid

   #118302

   Purpose

   Expression of mouse C-Fos

   Depositor

   Philip Stork

   Article

   Pellegrino et al J Neurochem. 2006 Dec;99(6):1480-93. doi

   Insert

   C-Fos S374A (Fos Mouse)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   S374A

   Promoter

   Available since

   Nov. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pENTR-actin-V159N

   Plasmid

   #118383

   Purpose

   Gateway compatible donor vector with actin-V159N.

   Depositor

   Maria Vartiainen

   Article

   Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

   Insert

   actin beta (ACTB Human)
   

   Use

   Donor-plasmid

   Tags

   Expression

   Mutation

   V159N

   Promoter

   Available since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. GFP-RanV19 E1RA2

    Plasmid

    #118317

    Purpose

    Expression of human Ran

    Depositor

    Philip Stork

    Article

    Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

    Inserts

    RanV19 (RAN Human)
    EPac1RA2 (RAPGEF3, RAPGEF4 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    in-frame insertion of RanV19 into GFPEpac1RA2 and…

    Promoter

    Available since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Flag-Rap1b S179AS180A

    Plasmid

    #118326

    Purpose

    Expression of bovine Rap1b

    Depositor

    Philip Stork

    Article

    Takahashi et al J Biol Chem. 2013 Sep 27;288(39):27712-2

    Insert

    Rap1b S179AS180A (RAP1B Bovine)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S179AS180A

    Promoter

    Available since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pENTR-actin-G168D,Y169D

    Plasmid

    #118384

    Purpose

    Gateway compatible donor vector with actin-G168D,Y169D.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Insert

    actin beta (ACTB Human)
    

    Use

    Donor-plasmid

    Tags

    Expression

    Mutation

    G168D,Y169D

    Promoter

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. hHSF1 RNAi resistant

    Plasmid

    #118347

    Purpose

    This plasmid expresses RNAi resistance version of hHSF1 to use for rescue experiments with HSF1 shRNA vector (plasmid #118345).

    Depositor

    Lea Sistonen

    Article

    Ostling et al J Biol Chem. 2007 Mar 9;282(10):7077-86.

    Insert

    hHSF1 (HSF1 Human)
    

    Use

    Tags

    myc and 6xHIS

    Expression

    Mammalian

    Mutation

    RNAi resistance mutations (does not change amino …

    Promoter

    Available since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pENTR-KAT14-547-782

    Plasmid

    #118376

    Purpose

    Gateway compatible donor vector with KAT14-547-782 truncation.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Insert

    KAT14 (KAT14 Human)
    

    Use

    Donor-plasmid

    Tags

    Expression

    Mutation

    Truncation (aa 547-782)

    Promoter

    Available since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSADuet779

    Plasmid

    #118395

    Purpose

    analysis of cis-splicing by mini inteins

    Depositor

    Hideo Iwai

    Article

    Iwai et al J Mol Biol. 2017 Dec 8;429(24):3942-3956

    Insert

    MjaTFIIB mini intein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBHDuet61

    Plasmid

    #118396

    Purpose

    analysis of cis-splicing by mini inteins

    Depositor

    Hideo Iwai

    Article

    Iwai et al J Mol Biol. 2017 Dec 8;429(24):3942-3956

    Insert

    MjaTFIIB mini intein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBHDuet50K

    Plasmid

    #118399

    Purpose

    analysis of cis-splicing by mini inteins

    Depositor

    Hideo Iwai

    Article

    Iwai et al J Mol Biol. 2017 Dec 8;429(24):3942-3956

    Insert

    MvuTFIIB mini intein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    incorporation of oligonucleotide during cloning

    Promoter

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBHDuet45

    Plasmid

    #118393

    Purpose

    analysis of cis-splicing by mini inteins

    Depositor

    Hideo Iwai

    Article

    Iwai et al J Mol Biol. 2017 Dec 8;429(24):3942-3956

    Insert

    MjaTFIIB mini intein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBHDuet173

    Plasmid

    #118398

    Purpose

    analysis of cis-splicing by mini inteins

    Depositor

    Hideo Iwai

    Article

    Iwai et al J Mol Biol. 2017 Dec 8;429(24):3942-3956

    Insert

    MvuTFIIB mini intein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBHDuet33

    Plasmid

    #118397

    Purpose

    analysis of cis-splicing

    Depositor

    Hideo Iwai

    Article

    Iwai et al J Mol Biol. 2017 Dec 8;429(24):3942-3956

    Insert

    MvuTFIIB intein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge