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Plasmid#164223PurposeExpression of membrane-localized mCherry driven by the ser-2promo3 promoter. For use as a PVD neuron marker in C. elegans.DepositorInsertmyr-mCherry
UseTagsExpressionWormMutationPromoterser-2promo3Available sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
RIP-Timer (DM#285)
Plasmid#15109DepositorInsertinsulin II promoter (Ins2 Rat)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 18, 2007AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046920
Plasmid#145717PurposeBacterial Expression plasmid for SARS-CoV-2 nsp3DepositorInsertSARS-CoV-2 nsp3 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;6xHISExpressionBacterialMutationEscherichia coli recode 1PromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 27, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSEVA221MBPc-AID-T7RNAP
Plasmid#167975PurposeExpresses fusion protein MBPc-AID-T7RNAP in E. coliDepositorInsertMBPc-AID-T7RNAP
UseTagsMBPc and T7RNAPExpressionBacterialMutationPromoterTetR-PtetAvailable sinceJune 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE- TRE-DEST-NBioTAP
Plasmid#53568PurposeLentiviral destination vector for N -Bio-1xproteinA constructs using Gateway recombination System. Design for cDNA clones from the CCSBHuman Orfeome Collection.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceAug. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
ibpAB-GFP
Plasmid#109390PurposeGFP cassette under the control of the ibpAB sigma32-responsive promoter. Used to test burden-responsive behaviour of the wt genomic promoter. The plasmid has got ColE1 origin of replication and AmpR.DepositorInsertpibpAB-GFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC5
Plasmid#104779PurposepSC218GG-Glycine max Ubiquitin:Gateway expression cassette binary vectorDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJan. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
miniCMV eGFP hPGK BSD
Plasmid#188519PurposeBackbone for CRISPR activation (CRISPRa) isogenic PAM testingDepositorTypeEmpty backboneUseLentiviralTagseGFPExpressionMammalianMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
MAC-N-GFP
Plasmid#172974PurposeMAC-tagged GFP expressionDepositorInsertGFP
UseTagsMAC-tagExpressionBacterial and MammalianMutationPromoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCiPS-NIFV
Plasmid#110807PurposeNon-Integrating Foamy Virus pol-expressing helper plasmid with mutations in the DDE catalyic core motif of the integrase sequence, remaining as unintegated episomes in transduced cellsDepositorInsertPol
UseTagsExpressionMammalianMutation2 mutations in the DDE catalyic core motif of the…PromoterCMVAvailable sinceJune 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTS94 pHAGE2 CMVtetO2 GFP-22xGS-3C-MCS
Plasmid#199347PurposeLentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-3C-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsEGFP-3CExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-YWHAE
Plasmid#31562DepositorInserttyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon (YWHAE Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGBW-m4046955
Plasmid#145616PurposeBacterial Expression plasmid for RNA-dependent RNA polymeraseDepositorInsertRNA-dependent RNA polymerase (Nsp12) (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
UseTagsCleavable TEV;FLAG_2ExpressionBacterialMutationwtPromoterPromoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…Available sinceApril 29, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNP1 sfGFP toehold switch
Plasmid#107355PurposeExpresses sfGFP (a GFP) in Ecoli off a T7 promoter when pNP1 sfGFP trigger is co-expressedDepositorInserttoehold 7 + sfGFP
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLNC Wnt-3aHA
Plasmid#18030DepositorInsertWnt-3a (Wnt3a Mouse)
UseRetroviralTagsHAExpressionMutationPromoterAvailable sinceJuly 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
Sender plasmid
Plasmid#127677PurposeEncodes the synthase LuxI for the quorum-sensing molecule C6-HSL.DepositorInsertLuxI
UseSynthetic BiologyTagsLVAExpressionBacterialMutationPromoterpT7Available sinceSept. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lsh_C2c2_WT
Plasmid#83487PurposeBacterial expression of WT L. shahii C2c2 CRISPR effectorDepositorInsertLsh_C2c2_WT
UseCRISPRTagsHis6-MBPExpressionBacterialMutationPromoterAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-MCM6-C-19
Plasmid#54171PurposeLocalization: Nucleus, Excitation: 487, Emission: 509DepositorInsertMCM6
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJuly 10, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
LbdCas12a KRAB
Plasmid#188503PurposeExpresses FLAG tagged LbdCas12a KRABDepositorInsertdCas12a
UseCRISPR and LentiviralTagsExpressionMammalianMutationD832APromoterEF1aAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
p6640 MSCV-P C-FlagHA 16E7-Kozak
Plasmid#35018DepositorInsertHPV16 E7 (E7 HPV 16)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationPromoterMSCV LTRAvailable sinceMarch 14, 2012AvailabilityAcademic Institutions and Nonprofits only