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Addgene

RIP-Timer (DM#285)
(Plasmid #15109)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 15109 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pTimer-1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4100
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    insulin II promoter
  • Alt name
    insulin II
  • Alt name
    ins2
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    700
  • GenBank ID
    J00748
  • Entrez Gene
    Ins2 (a.k.a. CP-II)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SacI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer na
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

See Author's Map for more information.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RIP-Timer (DM#285) was a gift from Douglas Melton (Addgene plasmid # 15109 ; http://n2t.net/addgene:15109 ; RRID:Addgene_15109)
  • For your References section:

    Adult pancreatic beta-cells are formed by self-duplication rather than stem-cell differentiation. Dor Y, Brown J, Martinez OI, Melton DA. Nature. 2004 May 6. 429(6987):41-6. 10.1038/nature02520 PubMed 15129273