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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 15109 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepTimer-1
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 4100
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameinsulin II promoter
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Alt nameinsulin II
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Alt nameins2
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SpeciesR. norvegicus (rat)
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Insert Size (bp)700
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GenBank IDJ00748
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Entrez GeneIns2 (a.k.a. CP-II)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SacI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer na (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
See Author's Map for more information.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RIP-Timer (DM#285) was a gift from Douglas Melton (Addgene plasmid # 15109 ; http://n2t.net/addgene:15109 ; RRID:Addgene_15109) -
For your References section:
Adult pancreatic beta-cells are formed by self-duplication rather than stem-cell differentiation. Dor Y, Brown J, Martinez OI, Melton DA. Nature. 2004 May 6. 429(6987):41-6. 10.1038/nature02520 PubMed 15129273