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Plasmid#15823DepositorInsertCullin5 (CUL5 Human)
UseTagsFlagExpressionMammalianMutationDominant negative: N441PromoterAvailable sinceSept. 28, 2007AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHAhFXR1
Plasmid#48694PurposeExpresses hFXR1DepositorInsertFlagHA FXR1 (isoform b) (FXR1 Human)
UseTagsFLAG and HAExpressionMammalianMutationPromoterCMV, 2x TetAvailable sinceJune 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBluescript-PB
Plasmid#133885PurposepiggyBac ITR's in high copy number pBluescript backbone plasmidDepositorTypeEmpty backboneUsePiggybacTagsExpressionMutationPromoterAvailable sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPPAT-EGFP
Plasmid#99105PurposeExpresses human PPAT-EGFP fusion in mammalian cellsDepositorInsertPPAT (PPAT Human)
UseTagsEGFP (enhanced green fluorescent protein)ExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) mCherry
Plasmid#176301PurposeExpresses mCherry mRNA from CMV promoterDepositorInsertmCherry
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-syn-jGCaMP7f-WPRE (AAV8)
Viral Prep#104488-AAV8PurposeReady-to-use AAV8 particles produced from pGP-AAV-syn-jGCaMP7f-WPRE (#104488). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP7f-WPRE plasmid DNA. Synapsin-driven GCaMP7f calcium sensor. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
hBax C3-EGFP
Plasmid#19741DepositorInsertBax (BAX Human)
UseTagsGFPExpressionMammalianMutationnonePromoterAvailable sinceNov. 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
ER-M-TAG (pGS555)
Plasmid#160040PurposepLV-A4-UCOE-EF1a-MMGT1-mScarlet-3xHA-IRES-BLAS-2A-SNAP-WPRE-A2DepositorInsertMMGT1-mScarlet-3xHA
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
PX458-AAVS1
Plasmid#113194PurposeEncodes gRNA for human AAVS1DepositorUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pILGFPE9
Plasmid#83545PurposeUsed to evaluate the expression output of TEF1 promoter with yEGFP used as the reporter gene.DepositorInsertTEF1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterTEF1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 TFAM-mScarlet
Plasmid#129573PurposeExpression of mScarlet fused TFAMDepositorInsertTFAM (TFAM Human)
UseTagsExpressionMammalianMutationNonePromoterAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pJMP1333
Plasmid#119268Purposerfp "test" strainDepositorInsertsgRNA NT1/RR1 (rfp)
UseTagsExpressionBacterialMutationD10A and H840APromoterAvailable sinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-UAS-CITRINE
Plasmid#104839PurposeSPARK reporter geneDepositorInsertCitrine
UseAAVTagsExpressionMammalianMutationPromoterUASAvailable sinceJan. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpRY-HF1-P2A-EGFP (RTW5008)
Plasmid#139997PurposeCMV and T7 promoter expression plasmid for human codon optimized SpRY-HF1(SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized SpCas9 variant named SpRY-HF1 with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…PromoterCMV and T7Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-32a(+)-NRG1B1
Plasmid#120287PurposeProduce human recombinant NRG1 in DE3 cellsDepositorInsertNRG1-beta 1
UseTags6x His and S-TagExpressionBacterialMutationCodon optimized for E. Coli productionPromoterT7 promoterAvailable sinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Lyn11-FRB
Plasmid#155228Purposemembrane anchor for FKBP-FRB systemDepositorInsertMembrane targeted (through Lyn11) FRB for use with FKBP-FRB dimerization system
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pRDA_355
Plasmid#187159PurposeLentiviral expression of doxycycline inducible customizable Sp Cas9 sgRNADepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only