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Plasmid#120978PurposeMoClo golden gate assembly CD part for TraR(W) (improved TraR quorum-sensing activator from DOI: 10.1021/acssynbio.5b00286). Please see Supplemental Documents for annotated Genbank file.DepositorInsertTraR(W) from Scott and Hasty 2016
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
P12m
Plasmid#120938PurposeMoClo golden gate assembly AB part for pLas-TetO (Promoter activated by LasR and repressed by TetR; encodes <3OC12 AHL AND aTc> logic). Please see Supplemental Documents for annotated Genbank file.DepositorInsertohAB carb pLasTetO
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
P15m
Plasmid#120939PurposeMoClo golden gate assembly AB part for pCin-TetO (Promoter activated by CinR and repressed by TetR; encodes <3OC14 AHL AND aTc> logic). Please see Supplemental Documents for annotated Genbank file.DepositorInsertohAB carb pCinTetO
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
P16m
Plasmid#120940PurposeMoClo golden gate assembly AB part for pCin-LacO (Promoter activated by CinR and repressed by LacI; encodes <3OC14 AHL AND IPTG> logic). Please see Supplemental Documents for annotated Genbank file.DepositorInsertohAB carb pCinLacO m
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
P32m
Plasmid#120943PurposeMoClo golden gate assembly AB part for PlacO (common LacI-repressed promoter; see DOI: 10.1126/science.1256272). Please see Supplemental Documents for annotated Genbank file.DepositorInsertPlacO (Tim Lu, Matt Bennett, etc)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
P33m
Plasmid#120944PurposeMoClo golden gate assembly AB part (medium-strength Mutalik library promoter; see DOI: 10.1038/nmeth.2404). Please see Supplemental Documents for annotated Genbank file.DepositorInsertP11 from Mutalik library (medium)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
P34m
Plasmid#120945PurposeMoClo golden gate assembly AB part (low-strength Mutalik library promoter; see DOI: 10.1038/nmeth.2404). Please see Supplemental Documents for annotated Genbank file.DepositorInsertP13 from Mutalik library (weak)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pX330-Flag-HFSpCas9-H840A
Plasmid#80455Purposehigh fidelity SpCas9 mammalian expression vectorDepositorInsertHFSpCas9_H840A
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationPromoterCBhAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
5CBD
Plasmid#69970PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertPVX_084705:MAC04G-C11:C228845
UseBaculovirus expressionTagsHis and TEV cleavage siteExpressionMutationPromoterpolyhedrinAvailable sinceOct. 7, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
5C4Q
Plasmid#69963PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertLdBPK_363130.1:MAC055-F03:C235029
UseTagsHis and TEV cleavage siteExpressionBacterialMutationPromoterT7-lacOAvailable sinceOct. 7, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEGFP-C1-NuMA
Plasmid#81029PurposeExpresses human NuMA with N-terminal EGFP tag in mammalian cellsDepositorInsertHomo sapiens nuclear mitotic apparatus protein 1 (NUMA1) (NUMA1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
XLone-Puro eGFP
Plasmid#140027PurposeTunable and temporal expression control of nuclear eGFPDepositorInsertEnhanced Green Fluorescent Protein
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-BG4-NLS-mVen
Plasmid#211449Purposemammalian expression anti-G4-DNA antibody BG4 C-terminally fused to nuclear localization sequenceDepositorInsertAnti-DNA G-quadruplex single chain antibody BG4
UseTagsNLS-mVenusExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CB-FLPe-P2A-tdTomato
Plasmid#72259PurposeExpress FLPe and tdTomato under CB promoterDepositorInsertFLPe-P2A-tdTomato
UseLentiviralTagsExpressionMammalianMutationT230I in tdTomato (please see depositor's co…PromoterCB promoter and chicken beta-actin + CMV enhancerAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pExp-sfGFP
Plasmid#112573PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and sfGFP (superfolder green fluorescent protein) fusions with optional C-terminal StrepII-tagDepositorTypeEmpty backboneUseTags8xHis-sfGFP and Strep tagExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
Cre Shine
Plasmid#37404PurposeThis is a 2A peptide construct that produces two different proteins from a single open reading frame, a nuclear targeted Cre recombinase, and a membrane targeted GFP.DepositorInsertCre Shine
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEYFP-C1-NuMA
Plasmid#28238PurposeExpresses human NuMA with N-terminal EYFP tag in mammalian cellsDepositorInsertHomo sapiens nuclear mitotic apparatus protein 1 (NUMA1) (NUMA1 Human)
UseTagsEYFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAS1-rab3P::his-24::mNeptune
Plasmid#124350PurposeNuclear Localized Pan-Neuronal mNeptuneDepositorInsertrab-3, mNeptune (rab-3 Nematode)
UseTagsExpressionWormMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
C39m
Plasmid#120972PurposeMoClo golden gate assembly CD part for ccdB (DNA gyrase toxin, modified synonymously to remove BsaI cut site; ccdA/C79m is antitoxin). Please see Supplemental Documents for annotated Genbank file.DepositorInsertccdB toxin
UseSynthetic BiologyTagsExpressionMutationBsaI site removedPromoterAvailable sinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
mEos3.2-Nup50-N-10
Plasmid#57479PurposeLocalization: Nuclear Pore, Excitation: 507 / 572, Emission: 516 / 580DepositorInsertNup50 (NUP50 Human)
UseTagsmEos3.2ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits