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Showing: 7841 - 7860 of 15032 results

  3. pGuide-P1-O1a (EL702)

    Plasmid
    #140040
    Purpose
    CRISPR DuMPLING negative control plasmid with probe/barcode P1 and negative control lacO1array spacer in the sgRNA. Also template for library PCRs.
    Depositor
    Johan Elf
    Article
    Camsund et al Nat Methods. 2020 Jan;17(1):86-92. doi:
    Insert
    barcode P1 and sgRNA lacO1 array
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    140040-plasmid-map-sequence-id-292294

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  12. pTX203

    Plasmid
    #89267
    Purpose
    Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsDEP1 gene, OsDEP1-gRNA02
    Depositor
    Daniel Voytas
    Article
    Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:
    Insert
    OsDEP1-gRNA02
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Plant
    Mutation
    Promoter
    Maize Ubiquitin1 promoter
    Available since
    May 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    89267-plasmid-map-sequence-id-177894

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  13. pTX202

    Plasmid
    #89266
    Purpose
    Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsDEP1 gene, OsDEP1-gRNA01
    Depositor
    Daniel Voytas
    Article
    Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:
    Insert
    OsDEP1-gRNA01
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Plant
    Mutation
    Promoter
    Maize Ubiquitin1 promoter
    Available since
    May 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    89266-plasmid-map-sequence-id-177842

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  14. pTX200

    Plasmid
    #89264
    Purpose
    Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsYSA gene, OsYSA-gRNA01
    Depositor
    Daniel Voytas
    Article
    Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:
    Insert
    OsYSA-gRNA01
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Plant
    Mutation
    Promoter
    Maize Ubiquitin1 promoter
    Available since
    May 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    89264-plasmid-map-sequence-id-177839

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  15. pTX199

    Plasmid
    #89263
    Purpose
    Express STU Cas9 with Maize Ubiquitin1 promoter in rice targeting OsPDS gene, OsPDS-gRNA02
    Depositor
    Daniel Voytas
    Article
    Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:
    Insert
    OsPDS-gRNA02
    Use
    CRISPR
    Tags
    SV40 NLS
    Expression
    Plant
    Mutation
    Promoter
    Maize Ubiquitin1 promoter
    Available since
    May 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    89263-plasmid-map-sequence-id-177838

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  16. pCas56

    Plasmid
    #82394
    Purpose
    sgRNA targeting YFP +46 from TSS; template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance
    Depositor
    Brian Pfleger
    Article
    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(
    Insert
    gRNA
    Use
    Tags
    Expression
    Mutation
    Promoter
    PEZ3
    Available since
    Sept. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    82394-plasmid-map-sequence-id-162297

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  17. pCas55

    Plasmid
    #82393
    Purpose
    sgRNA targeting YFP +172 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance
    Depositor
    Brian Pfleger
    Article
    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(
    Insert
    gRNA
    Use
    Tags
    Expression
    Mutation
    Promoter
    PEZ3
    Available since
    Sept. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    82393-plasmid-map-sequence-id-162303

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  18. pU6_(GLuc)_INT[S1Apt]

    Plasmid
    #68425
    Purpose
    Transient expression in mammalian cells of an "INT" construct_bearing the "S1" streptavidin aptamer, targeting the GLuc reporter. U6 promoter
    Depositor
    John Rinn
    Article
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    INT construct with S1 streptavidin aptamer
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    human U6
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68425-plasmid-map-sequence-id-120203

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  19. pXPR_045

    Plasmid
    #107144
    Purpose
    cloning of a sgRNA
    Depositor
    David Root
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    107144-plasmid-map-sequence-id-205294

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  20. pSBtet-Hyg-dCas9-KRAB-MeCP2_hU6-SapI

    Plasmid
    #196082
    Purpose
    (Empty Backbone) Inducible CRISPRi vector conferring hygromycin B resistance, ready for insertion of gRNA targeting gene of interest using SapI restriction sites.
    Depositor
    Joanna Bereta
    Article
    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100
    Inserts
    dCas9-KRAB-MeCP2 repressor
    hU6 promoter and gRNA scaffold
    Aminoglycoside phosphotransferase
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Catalytically dead mutant of the Cas9 endonucleas…
    Promoter
    Available since
    March 30, 2023
    Availability
    Academic Institutions and Nonprofits only
    196082-plasmid-map-sequence-id-388978

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Showing: 7841 - 7860 of 15032 results