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ClhN-p500-Erg6-V5-APEX2-URA
Plasmid#207070PurposeExpressing p500-Erg6-V5-APEX2. Stains cell peripheral in addition to lipid droplets.DepositorInsertErg6
UseTagsV5-APEX2ExpressionYeastMutationPromoterpERG6Available sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-pADH3-GFP-Pex3-4V5-APEX2-URA
Plasmid#207064PurposeExpressing GFP-Pex3-4V5-APEX2 under ADH3 promoter. Insufficient signal for EM.DepositorInsertPex3
UseTags4xV5-APEX2 and GFPExpressionYeastMutationPromoterpADH3Available sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-p1k-OM14-V5-APEX2-Ura
Plasmid#207073PurposeExpressing OM14-V5-APEX2. Marker for mitochondrial outer membrane.DepositorInsertOM14
UseTagsV5-APEX2ExpressionYeastMutationPromoterpOM14Available sinceJune 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSCL452
Plasmid#219502PurposeGolden Gate entry vector for library cloning of msd-sgRNA arrays: GAL7p-Csy4-PaqCI-PaqCI-GAL7t // SNR52p-msr-SUP4tDepositorInsertGAL7p-Csy4-PaqCI-PaqCI-GAL7t // SNR52p-msr-SUP4t
UseTagsExpressionYeastMutationPromoterGAL7Available sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKL1-100
Plasmid#219408PurposeReporter gene (4 operators) for zinc finger activatorDepositorInsert(42-10 op)x4-minCyc1-Venus-tCyc1
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKL1-422
Plasmid#219410PurposeReporter gene (1 operator) for zinc finger activatorDepositorInsert(42-10 op)x1-minCyc1-Venus-tCyc1
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKL1-424
Plasmid#219412PurposeReporter gene (3 operator) for zinc finger activatorDepositorInsert(42-10 op)x3-minCyc1-Venus-tCyc1
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKL1-095
Plasmid#219407PurposeReporter gene (4 operators) for zinc finger activatorDepositorInsert(13-6 op)x4-minCyc1-Venus-tCyc1
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKL1-423
Plasmid#219411PurposeReporter gene (2 operator) for zinc finger activatorDepositorInsert(42-10 op)x2-minCyc1-Venus-tCyc1
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-p1k-Cox4-V5-APEX2-Ura
Plasmid#207061PurposeExpressing Cox4-V5-APEX2. Marker for mitochondrial inner membrane. Tends to generate facture in thin resin sections.DepositorInsertCox4
UseTagsV5-APEX2ExpressionYeastMutationPromoterpCOX4Available sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-p1k-Cox4-GFP-APEX2-Ura
Plasmid#207062PurposeExpressing Cox4-GFP-APEX2. GFP interferes with APEX staining.DepositorInsertCox4
UseTagsGFP-APEX2ExpressionYeastMutationPromoterpCOX4Available sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-pADH3-Snf7-4V5-APEX2-URA
Plasmid#207065PurposeModerate overexpression of Snf7-4V5-APEX2 under ADH3 promoter. Marker for late endosomes. Only partially functional.DepositorInsertSnf7
UseTags4xV5-APEX2ExpressionYeastMutationPromoterpADH3Available sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-pERG6-Pex3-4V5-APEX2-URA
Plasmid#207068PurposeModerate overexpression of Pex3-4V5-APEX2 under ERG6 promoter. Marker for peroxisomes.DepositorInsertPex3
UseTags4xV5-APEX2ExpressionYeastMutationPromoterpERG6Available sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-pLSP1-Pex3-4V5-APEX2-URA
Plasmid#207069PurposeModerate overexpression of Pex3-4V5-APEX2 under LSP1 promoter. Marker for peroxisomes.DepositorInsertPex3
UseTags4xV5-APEX2ExpressionYeastMutationPromoterpLSP1Available sinceMarch 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-pTPI1-Emc1-GFP-APEX2-Ura
Plasmid#207074PurposeExpressing Emc1-GFP-APEX2. GFP interferes with APEX staining.DepositorInsertEmc1
UseTagsGFP-APEX2ExpressionYeastMutationPromoterpTPI1Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
ClhN-pTPI1-Cox4-GFP-APEX2-Ura
Plasmid#207075PurposeExpressing Cox4-GFP-APEX2. GFP interferes with APEX staining.DepositorInsertCox4
UseTagsGFP-APEX2ExpressionYeastMutationPromoterpTPI1Available sinceFeb. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDN0604 (pDEST, HygR)
Plasmid#210483PurposepDEST vector with HygR marker for gene expression in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…TagsExpressionYeastMutationPromoterADH1Available sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDN0603 (pDEST, NatR)
Plasmid#210482PurposepDEST vector with NatR marker for gene expression in S. cerevisiae.DepositorTypeEmpty backboneUseSynthetic Biology; Destination vector for gateway…TagsExpressionYeastMutationPromoterADH1Available sinceDec. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNZ18-CAH
Plasmid#203159PurposeReplicates in E. coli, S. cerevisiae, and A. laidlawiiDepositorInsertYeast centromere, autonomously replicating sequence, histidine auxotrophic marker
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only
