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Plasmid#115202PurposeLentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291D/S293D into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')DepositorInsertCR (CRISPR/Cas9-resistant)-PDHA2S291D/S293D (PDHA2 Human)
UseLentiviralTagsExpressionMutationc.321C>T (silent when translated to protein, c…PromoterEF1alphaAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
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Lenti-sgZmat3.51.5/Cre
Plasmid#167855PurposeExpresses Cre recombinase and an sgRNA targeting Zmat3DepositorInsertgRNA targeting Zmat3 (Zmat3 Mouse)
UseCRISPR, Cre/Lox, Lentiviral, and Mouse TargetingTagsExpressionMammalianMutationPromoterAvailable sinceMay 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
61427-sgHtt_10
Plasmid#157861PurposeExpresses a sgRNA targeting mouse Htt promoter for gene activation using the SAM systemDepositorInsertHtt (Htt Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
61427-sgHtt_6
Plasmid#157860PurposeExpresses a sgRNA targeting mouse Htt promoter for gene activation using the SAM systemDepositorInsertHtt (Htt Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEN487 - Sororin-AID[71-114]-eGFP-FRT-Blast-FRT targeting conuct
Plasmid#156433PurposeTargeting vector to introduce an AID-eGFP cassette at the mouse Cdca5 (SORORIN) locus using BLASTICIDIN selection. Auxin-inducible degron system. Designed to be used with sgRNA GGGATGCCCGTCATTAAGTGDepositorInsertSORORIN, AID (Cdca5 Mouse)
UseMouse TargetingTagsExpressionMammalianMutationnonePromoterAvailable sinceSept. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Blast-sgMARK3
Plasmid#138706PurposeExpresses a human MARK3-targeting sgRNA and Cas9DepositorInsertsgMARK3 human (MARK3 Human)
UseLentiviralTagsExpressionMutationPromoterhU6Available sinceMarch 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Puro-V2.0-sg1HsTNRC6A_AH
Plasmid#148849PurposeMammalian Expression of HsTNRC6A-sgRNA1DepositorInsertHsTNRC6A-sgRNA1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Puro-V2.0-sg1HsTNRC6B_AH
Plasmid#148850PurposeMammalian Expression of HsTNRC6B-sgRNA1DepositorInsertHsTNRC6B-sgRNA1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Puro-V2.0-sg2HsTNRC6A_AH
Plasmid#148852PurposeMammalian Expression of HsTNRC6A-sgRNA2DepositorInsertHsTNRC6A-sgRNA2
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Puro-V2.0-sg3HsTNRC6B_AH
Plasmid#148853PurposeMammalian Expression of HsTNRC6B-sgRNA3DepositorInsertHsTNRC6B-sgRNA3
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKLV-gRNA-CTBP2-MGMT_1
Plasmid#136419PurposeLentiviral expression of gRNAs targeting intron 1 of human CTBP2 and intron 1 of human MGMT. Also constitutively expresses Puromycin fused to TagBFP.DepositorInsertU6_sgRNA(CTBP2)_U6_sgRNA(MGMT)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Puro-V2.0-sg1HsAGO2_AH
Plasmid#148848PurposeMammalian Expression of HsAGO2-sgRNA1DepositorInsertHsAGO2-sgRNA1 (AGO2 S.pyogenes)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Puro-V2.0-sg2HsAGO2_AH
Plasmid#148851PurposeMammalian Expression of HsAGO2-sgRNA2DepositorInsertHsAGO2-sgRNA2 (AGO2 S.pyogenes)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKLV-gRNA-BTRC-MGMT_2
Plasmid#136416PurposeLentiviral expression of gRNAs targeting intron 2 of human BTRC and intron 1 of human MGMT. Also constitutively expresses Puromycin fused to TagBFP.DepositorInsertU6_sgRNA(BTRC)_U6_sgRNA(MGMT)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWT047c
Plasmid#107903PurposeW2.7-1 plasmid. Contains PR-sd5-BE2, PLac-sgRNA1 and is part of CAMERA 2.7DepositorInsertPR-sd5-BE2, PLac-sgRNA1
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWT021a
Plasmid#107895PurposeW2.0 plasmid. Contains PTetO-SD8-BE2, PLac-sgRNA1 and is part of CAMERA 2.0DepositorInsertPTetO-SD8-BE2, PLac-sgRNA1
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWT046a
Plasmid#107897PurposeW2.2, W2.4-1 plasmid. Contains PTetO-SD8-BE2, PBAD-sgRNA3 and is part of CAMERA 2.2 and 2.4DepositorInsertPTetO-SD8-BE2, PBAD-sgRNA3
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWT037a
Plasmid#107896PurposeW2.1 plasmid. Contains PTetO-SD8-BE2, PLacO-sgRNA1 and is part of CAMERA 2.1DepositorInsertPTetO-SD8-BE2, PLacO-sgRNA1
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only