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Plasmid#138108PurposeGolden Gate recipient and Gateway entry vector; assembly of 4 gRNAs driven by Zea mays Ubi promoterDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceMarch 31, 2021AvailabilityAcademic Institutions and Nonprofits only
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pCSII-EF-miRFP670v1-hGem(1/110)
Plasmid#80006PurposehGem(1/110) fused with near-infrared fluorescent protein miRFP670v1 (S/G2/M phase cell cycle reporter)DepositorInserthGeminin (1/110) (GMNN Human)
UseLentiviralTagsmiRFP670v1ExpressionMutationPromoterEF-1 alphaAvailable sinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMito-miRFP703
Plasmid#80000PurposeMitochondria labeling with near-infrared fluorescent protein miRFP703DepositorInsertcytochrome C oxidase subunit VIII (COX8A Human)
UseTagsmiRFP703ExpressionMammalianMutationPromoterCMVAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.3 HA-hTSPAN12
Plasmid#115784PurposeExpresses human TSPAN12 with N-terminal HA-tag in mammalian cells. Contains multiple silent mutations that introduce restriction sites.DepositorInsertTSPAN12 (TSPAN12 Human)
UseTagsHAExpressionMammalianMutationmultiple silent mutations introduce restriction s…PromoterCMVAvailable sinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lenti-sgp53/Cre
Plasmid#89646PurposeExpresses a p53-targeting gRNA and Cre-recombinaseDepositorInsertgRNA targeting p53 (Trp53 Mouse)
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
6His-MBP-TEV-huLbCpf1
Plasmid#90096PurposeBacterial expression plasmid for protein purificationDepositorInserthuLbCpf1
UseTags3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…ExpressionBacterialMutationPromoterAvailable sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-pAce-Kv2.1PR
Plasmid#195529PurposeGreen fluorescent, positive response-polarity voltage indicator under the control of CaMKII promoter; soma-targetedDepositorInsertpAce-Kv2.1 proximal restriction sequence
UseAAVTagsExpressionMammalianMutationAce-mNeon 78K, 81D, 92N, 178F; SY linkerPromoterCaMKIIAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-BLRR
Plasmid#158958PurposeBioluminescent repair reporter (BLRR) for DNA double strand break (DSB) repairs: homology-directed repair (HDR) and non-homologous end joining (NHEJ).DepositorInsertBLRR
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
FASTHDR-Cterm-HaloTag-Puromycin
Plasmid#167208PurposePlasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with HaloTag and selection with PuromycinDepositorTypeEmpty backboneUseCRISPRTagsHaloTagExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMAL-SH3(2)-5R
Plasmid#112089PurposeBacterial expression plasmid containing His and MBP tags for 5 repeats of the second SH3 domain from human NCK1.DepositorInsertSH3(2)-5R (NCK1 Human)
UseTagsHis6 and MBPExpressionBacterialMutationconstruct contains only repeats of the second SH3…PromoterLacAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCD5-PR8-TEV-sfGFP-twinStrep
Plasmid#157933PurposeExpresses influenza A virus trimeric hemagglutinin fused to a sfGFPDepositorInsertA/PuertoRico/8/34 hemagglutinin (HA Synthetic)
UseTagssfGFP & TwinStrepExpressionMammalianMutationE190DPromoterCMVAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
SuExp His-Myc-hPLD4
Plasmid#173852PurposeProtein expression plasmid for recombinant His-Myc tag human PLD4DepositorInsertPLD4 (PLD4 Human)
UseTags6xHis, Myc, Factor X cleavableExpressionMammalianMutationintracellular and transmembrane domain truncated,…PromoterCMVAvailable sinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-R-iLACCO1.2
Plasmid#208101PurposeBiosensor for intracellular L-lactateDepositorInsertR-diLACCO1
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28_6xHis-PARP1-CATdeltaHD
Plasmid#174811PurposeBacterial expression of a hyperactive PARP1 CAT lacking the autoinhibitory HD subdomainDepositorInsertPARP1-CATdeltaHD (PARP1 Human)
UseTags6xHisExpressionBacterialMutationResidues 678-787 replaced by eight-residue linker…PromoterAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-fDIO-pAce-Kv2.1PR
Plasmid#195535PurposeFlp recombinase-dependent green fluorescent, positive response-polarity voltage indicator; soma-targetedDepositorInsertpAce-Kv2.1 proximal restriction sequence
UseAAVTagsExpressionMammalianMutationAce-mNeon 78K, 81D, 92N, 178F; SY linkerPromoterCAGAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-hGABARAP
Plasmid#87871PurposeExpress a EGFP-human GABARAP in mammalian cellsDepositorInsertGABARAP (GABARAP Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-R-diLACCO1
Plasmid#208102PurposeControl biosensor for intracellular L-lactateDepositorInserteLACCO2.1
UseAAVTagsExpressionMutationPromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
SuExp His-Myc-mPLD3
Plasmid#173853PurposeProtein expression plasmid for recombinant His-Myc tag mouse PLD3DepositorInsertPLD3 (Pld3 Mouse)
UseTags6xHis, Myc, Factor X cleavableExpressionMammalianMutationintracellular and transmembrane domain truncated,…PromoterCMVAvailable sinceJan. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
Il12b 400bp pro-Luc
Plasmid#20020DepositorInsertIl12b promoter (Il12b Mouse)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
SuExp His-Myc-mPLD4
Plasmid#201185PurposeProtein expression plasmid for recombinant His-Myc tag mouse PLD4DepositorUseTagsHis-Myc, Factor Xa cleavableExpressionMammalianMutationcytosolic and transmembrane domain truncated; add…PromoterCMVAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only