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Addgene
Showing: 7821 - 7840 of 14137 results
  1. XLone-eGFP NLS (no drug resistant gene)

    Plasmid
    #140032
    Purpose
    Tunable and temporal expression control of eGFP (or gene of interest) with constitutive nuclear eGFP
    Depositor
    Insert
    Enhanced Green Fluorescent Protein
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Availability
    Academic Institutions and Nonprofits only
  2. pSBbi-Pur-AktAR2-D

    Plasmid
    #125196
    Purpose
    SB-transposon plasmid for stable expression of unphosphorylatable "dead" version of AktAR2 variant of FRET-based AKT activity reporter
    Depositor
    Insert
    AktAR2-D
    Use
    Transposon
    Tags
    6xHis, T7, and Xpress, mCerulean3 and cpVenus[E17…
    Expression
    Mammalian
    Mutation
    FoxO1 Thr-24 changed to Val rendering this inacti…
    Promoter
    EF1a/RPBSA
    Availability
    Academic Institutions and Nonprofits only
  3. pET32a-His-FADD

    Plasmid
    #78803
    Purpose
    To purify His-tagged recombinant FADD protein
    Depositor
    Insert
    GIG3 (FADD Human)
    Use
    Tags
    6x His, S-Tag, Thrombin cleavage site, and TrxA (…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
  4. 5E16 (PfPKG, PF14_0346)

    Plasmid
    #79518
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Insert
    PF14_0346
    Use
    Tags
    6xHis and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Availability
    Academic Institutions and Nonprofits only
  5. pYFP-NEAT1pr_v1-RT

    Plasmid
    #97087
    Purpose
    Encodes a HR repair template for knock-in a YFP expression cassette at the promoter of human NEAT1 gene. Best used with px330-NEAT1pr_v1 vector, but also works with px330-NEAT1pr_v2.
    Depositor
    Insert
    nuclear paraspeckle assembly transcript 1 (NEAT1 Human)
    Use
    CRISPR
    Tags
    EYFP
    Expression
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  6. NUDT22 (NUDT22A-c001)

    Plasmid
    #98230
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    NUDT22A (NUDT22 Human)
    Use
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
  7. NUDT4 (NUDT4A-c001)

    Plasmid
    #98241
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    NUDT4A (NUDT4 Human)
    Use
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
  8. ZMYND8 (PRKCBP1A-c097)

    Plasmid
    #98244
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    PRKCBP1A (ZMYND8 Human)
    Use
    Tags
    His6-Zb-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
  9. SRPK1 (SRPK1A-c100)

    Plasmid
    #98246
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    SRPK1A (SRPK1 Human)
    Use
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
  10. pVP16-tev-PRT1

    Plasmid
    #83405
    Purpose
    bacterial expression vector pVP16 containing Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Insert
    PROTEOLYSIS1 (PRT1) (PRT1 Mustard Weed, E. coli)
    Use
    Tags
    His8, MBP, and tev cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  11. pAc5.1B-EGFP-DmNot3-F764A-dsRNAres_Q

    Plasmid
    #147261
    Purpose
    Insect Expression of DmNot3-F764A-dsRNAres
    Depositor
    Insert
    DmNot3-F764A-dsRNAres (Not3 Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    one silent mutation compared to the sequence give…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  12. pAc5.1B-EGFP-DmNot3-F818A-dsRNAres_Q

    Plasmid
    #147262
    Purpose
    Insect Expression of DmNot3-F818A-dsRNAres
    Depositor
    Insert
    DmNot3-F818A-dsRNAres (Not3 Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    one silent mutation compared to the sequence give…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  13. pAc5.1B-EGFP-DmNot3-mut1-dsRNAres_Q

    Plasmid
    #147263
    Purpose
    Insect Expression of DmNot3-mut1-dsRNAres
    Depositor
    Insert
    DmNot3-mut1-dsRNAres (Not3 Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    one silent mutation compared to the sequence give…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  14. pAc5.1B-EGFP-DmNot3-mut2-dsRNAres_Q

    Plasmid
    #147264
    Purpose
    Insect Expression of DmNot3-mut2-dsRNAres
    Depositor
    Insert
    DmNot3-mut2-dsRNAres (Not3 Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    one silent mutation compared to the sequence give…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  15. CS2-H2B-EGFP

    Plasmid
    #132414
    Purpose
    To make synthetic mRNA encoding a nuclear EGFP
    Depositor
    Insert
    H2B-EGFP (H2BC21 Synthetic)
    Use
    Synthetic mrna production
    Tags
    Expression
    Mutation
    Promoter
    SP6
    Availability
    Academic Institutions and Nonprofits only
Showing: 7821 - 7840 of 14137 results