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Plasmid#17469DepositorInsertBnip3L RNAi (BNIP3L Human)
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceApril 18, 2008AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Akt(R25C)
Plasmid#39532DepositorInsertAkt(R25C) (Akt1 Mouse)
UseTags6G linker, EGFP, and HAExpressionMammalianMutationR25CPromoterAvailable sinceSept. 26, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pTSara-C
Plasmid#60722PurposeExpresses the C-terminal fragment of split T7 RNAP split a position 179. Insert is driven by the arabinose inducible promoter PBAD. Contains a constitutive araC ORFDepositorInsertResidues 180-880 of T7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationOnly amino acids 180-880 of T7 RNAPPromoterPBADAvailable sinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTSara-N
Plasmid#60721PurposeExpresses the N-terminal fragment of split T7 RNAP split a position 179. Insert is driven by PBAD. Contains a constitutive araC ORFDepositorInsertResidues 1-179 of T7 RNAP
UseSynthetic BiologyTagsExpressionBacterialMutationOnly amino acids 1-179 of T7 RNAPPromoterPBADAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-BG505.SOSIP-gp160
Plasmid#123273PurposeMammalian expression plasmid for Env from the BG505 HIV-1 isolate (containing SOSIP mutations)DepositorInsertHIV-1 (BG505) Env
UseTagsCD5 leader peptideExpressionMammalianMutationCodon-optimized synthetic gene; SOSIP mutationsPromoterCMVAvailable sinceApril 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET41a iGluf
Plasmid#119833PurposeProduction of recombinant iGluSnFR E25D fast variant, a single-wavelength glutamate indicator, in E. coliDepositorInsertiGluf
UseTagsGSTExpressionBacterialMutationE25DPromoterT7Available sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
LB595 (Bcl-2 promoter CRE mut)
Plasmid#15243DepositorInsertB-cell lymphoma/leukemia-2 (BCL2 Human)
UseLuciferaseTagsLuciferaseExpressionBacterialMutationMutated CRE site at -1537 changed from GTGACGTTA …PromoterAvailable sinceAug. 1, 2007AvailabilityAcademic Institutions and Nonprofits only -
pJL2
Plasmid#184130PurposeFor rescuing null mrp-1 mutation and labelling mrp-1 protein. Translational fusion construct. pPD95.75_Pmrp-1::mrp-1(isoform C cDNA)::gfp.DepositorInsertMRP-1 (mrp-1 Nematode)
UseTagsGFPExpressionWormMutationPromoterPmrp-1Available sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJL1
Plasmid#184129PurposeFor rescuing null mrp-1 mutation. pPD95.77_Pmrp-1::mrp-1(isoform C cDNA)::SL2::gfp. SL2 prevents fusion of MRP-1 and GFP.DepositorInsertMRP-1 (mrp-1 Nematode)
UseTagsGFPExpressionWormMutationPromoterPmrp-1 (3kb)Available sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pIhh742bp-Luc
Plasmid#53605PurposeReporter construct containing a 742 bp promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assaysDepositorInsertIhh promoter
UseLuciferase; ReporterTagsExpressionMutationPromoterAvailable sinceJune 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSR-siKlf5
Plasmid#41740DepositorInsertKlf5 (Klf5 Mouse)
UseRNAiTagsExpressionMutationPromoterAvailable sinceJan. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
MigR1-Flag-TRIB2
Plasmid#131154PurposeRetroviral expression of flag-tagged mouse TRIB2DepositorInsertTrib2 (Trib2 Mouse)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV5 Flag Jnk1a1(APY)
Plasmid#15742DepositorInsertJnk1a1(TPF) (MAPK8 Human)
UseTagsFlagExpressionMammalianMutationThr-190AlaPromoterAvailable sinceMarch 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRSET-A2-77p-Venus
Plasmid#64856PurposeExpresses aa 1594-1670 of A2 Domain of VWF protein, TEV cleavage site and Venus followed by 7X His tagDepositorInsertVWF A2 domain aa 1594-1670_TEV_Venus_7X His (VWF Human, Synthetic)
UseTags7X His and VenusExpressionBacterialMutationPromoterT7 PromoterAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-ERBB4 (E542K)
Plasmid#29539DepositorInsertERBB4 (ERBB4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 6, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRK-flag-USP19 494-1318
Plasmid#78587PurposeExpression flag-USP19 494-1318 in mammalian cellsDepositorInsertflag-USP19 494-1318 (USP19 Human)
UseTagsflagExpressionMammalianMutationdeleted amino acid 1-493PromoterCMVAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGBDU-Atg17
Plasmid#121394PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
UseTagsExpressionYeastMutationPromoterADH1 promoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSK226
Plasmid#129268Purposedual yTRAP, RNQ1 fusion to aTF2 reporting on [RNQ+] state with mNeonGreen reporterDepositorInsertRnq1
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-mIFITM1-C49,50,83,103A
Plasmid#58421PurposeExpresses murine IFITM1-C49,50,83,103A with an N-terminal HA tag in mammalian cells (cysteine-less mutant that is not palmitoylated).DepositorInsertIFITM1 (Ifitm1 Mouse)
UseTagsHAExpressionMammalianMutationCysteines 49, 50, 83, and 103 to AlaninePromoterCMV IEAvailable sinceSept. 9, 2014AvailabilityAcademic Institutions and Nonprofits only