Addgene

We narrowed to 1,391 results for: sfGFP

Showing: 761 - 780 of 1391 results

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pJBL6721

Plasmid

#139152

Purpose

PDNsp1 - TARGET8 - sfGFP

Depositor

Julius Lucks

Article

Glasscock et al ACS Synth Biol. 2021 May 21;10(5):1199-1

Insert

sfGFP

Use

Tags

Expression

Bacterial

Mutation

N/A

Promoter

Available since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

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pJBL6722

Plasmid

#139153

Purpose

Pstabilized - TARGET8 - sfGFP

Depositor

Julius Lucks

Article

Glasscock et al ACS Synth Biol. 2021 May 21;10(5):1199-1

Insert

sfGFP

Use

Tags

Expression

Bacterial

Mutation

N/A

Promoter

Available since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

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pMC0x_gRNA_Pos5b_Ptet

Plasmid

#179338

Purpose

gRNA Position Vector 5b, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 cam

Depositor

Anke Becker

Article

Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

Insert

Ptet gRNA with sfGFP dropout

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pMC0x_gRNA_Pos5a_Ptet

Plasmid

#179337

Purpose

gRNA Position Vector 5a, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 cam

Depositor

Anke Becker

Article

Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

Insert

Ptet gRNA with sfGFP dropout

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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DT039A

Plasmid

#159400

Purpose

Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli J23117 (weak) promoter

Depositor

Sheref Mansy

Article

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.J23117-sfGFP

Use

Synthetic Biology

Tags

FLAG

Expression

Bacterial

Mutation

Promoter

J23117 (weak) promoter

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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DT038A

Plasmid

#159399

Purpose

Constitutive superfolder GFP (sfGFP) expression, controlled under E.coli J23106 (medium) promoter

Depositor

Sheref Mansy

Article

Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

Insert

pr.J23106-sfGFP

Use

Synthetic Biology

Tags

FLAG

Expression

Bacterial

Mutation

Promoter

J23106 (medium) promoter

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pAV0772

Plasmid

#133516

Purpose

Expression of sfGFP tagged beta-tubulin to monitor microtubules

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pAde6(PmeI)-p(atb2)-sfGFP-atb2-terminator(atb2)-hphMX

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0605

Plasmid

#133503

Purpose

Expression of sfGFP targeted to the plasma membrane with an amphipathic helix of RitC

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pUra4(AfeI)-p(act1)-sfGFP-RitC-terminator(tdh1)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pBFC0687

Plasmid

#186692

Purpose

ShDART under control of Lac promoter, original configuration (pre-gRNA terminator and J23119 promoter), Sh_2xBsaI_NT gRNA, GmR+sfGFP cargo

Depositor

Jennifer Doudna

Article

Rubin et al Nat Microbiol. 2022 Jan;7(1):34-47. doi:

Inserts

tnsB
tnsC
tniQ
Cas12k
Sh_2xBsaI_NT (Guide Stuffer) gRNA
Tn7 transposon
GmR+sfGFP ShDART Cargo

Use

Tags

Expression

Bacterial

Mutation

Promoter

PLac

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pJG100

Plasmid

#138966

Purpose

Peft-3::wrmScarlet1-10::unc-54 3’UTR. Expresses wrmScarlet1-10 in somatic tissues of C. elegans

Depositor

Cynthia Kenyon

Article

Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

Insert

wrmScarlet1-10

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

July 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS_4xMma PylT_GFP 150TAG 6xHis

Plasmid

#140015

Purpose

Plasmid with 4xMmaPylT cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

sfGFP

Use

Tags

6xHis

Expression

Mammalian

Mutation

150TAG

Promoter

EF1

Available since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

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pBEL1092

Plasmid

#195635

Purpose

Expression/purification of 6xHis-TEV-sfGFP

Depositor

Gira Bhabha, Damian Ekiert

Article

MacRae et al J Biol Chem. 2023 Apr 24:104744. doi: 10

Insert

sfGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pNP555

Plasmid

#222722

Purpose

sfGFP-ON BsaI Golden Gate Landing Pad Backbone for RE-LE (bRNA in cis expression)

Depositor

Patrick Hsu

Article

Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

Type

Empty backbone

Use

Tags

sfGFP

Expression

Bacterial

Mutation

Promoter

Available since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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RINS1(mut)

Plasmid

#107291

Purpose

Plasmid for the expression of the inactive mutant of RINS1 sensor

Depositor

Dmytro Yushchenko

Article

Schifferer et al Cell Chem Biol. 2017 Apr 20;24(4):525-53

Insert

RINS1(mut)

Use

Tags

sfGFP

Expression

Mammalian

Mutation

two Arg/Ser mutations at the beginning and in the…

Promoter

CMV

Available since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pET14-GFP30_Encap

Plasmid

#86406

Purpose

Expresses sfGFP tagged with a 30 amino acid CLP and encapsulin as an operon

Depositor

David Savage

Article

Cassidy-Amstutz et al Biochemistry. 2016 Jun 21;55(24):3461-8.

Inserts

sfGFP with a 30aa CLP tag
Encapsulin

Use

Tags

6x Histidine

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pCB11

Plasmid

#201111

Purpose

pSR58.6 (Addgene plasmid #63176) modified to include the tetA gene upstream of sfGFP.

Depositor

Mary Dunlop

Article

Lugagne et al Nat Commun. 2024 Mar 8;15(1):2148. doi:

Inserts

CcaR
tetA(C)
sfGFP

Use

Tags

Expression

Bacterial

Mutation

C-to-T transition at pos. no. 1049 of tetA(C) lea…

Promoter

J23100 and PcpcG2-172

Available since

March 11, 2024

Availability

Academic Institutions and Nonprofits only

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pET14-GFP15_Encap

Plasmid

#86407

Purpose

Expresses sfGFP tagged with a 15 amino acid CLP and encapsulin as an operon

Depositor

David Savage

Article

Cassidy-Amstutz et al Biochemistry. 2016 Jun 21;55(24):3461-8.

Inserts

sfGFP with a 15aa CLP tag
Encapsulin

Use

Tags

6x Histidine

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pCK760

Plasmid

#192643

Purpose

Expresses BBa_J23110-sfGFP (capacity monitor) and J3-Bba_J23117-mRFP (CRISPRa reporter) on pSC101-AmpR

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

Insert

BBa_J23110-sfGFP

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

BBa_J23110

Available since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pET14_GFP5_Encap

Plasmid

#86408

Purpose

Expresses sfGFP tagged with a 5 amino acid CLP and encapsulin as an operon

Depositor

David Savage

Article

Cassidy-Amstutz et al Biochemistry. 2016 Jun 21;55(24):3461-8.

Inserts

sfGFP with a 5aa CLP tag
Encapsulin

Use

Tags

6x Histidine

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pCellFree_G10

Plasmid

#67144

Purpose

Gateway destination vector for cell-free expression with C-term sfGFP 8xHis tag

Depositor

Kirill Alexandrov

Article

Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

Type

Empty backbone

Use

Cell-free expression

Tags

HIS and sfGFP

Expression

Mutation

Promoter

Available since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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