Skip to main content
Addgene
Showing: 761 - 780 of 6305 results

  5. Carl-CeNL(Ca2+)_110μ-KDEL-pcDNA3

    Plasmid
    #111925
    Purpose
    Cyan color luminescent Ca2+ indicator targeted to endoplasmic reticulum. Calreticulin and a KDEL signal for ER retention located at the N-terminus and C-terminus of CeNL(Ca2+)_110μ
    Depositor
    Takeharu Nagai
    Article
    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/
    Insert
    CeNL(Ca2+)_110μ
    Use
    Tags
    Calreticulin and KDEL
    Expression
    Mammalian
    Mutation
    E31D, F92W, E104D. D133E at CaM
    Promoter
    CMV
    Available since
    June 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    111925-plasmid-map-sequence-id-214006

    Enlarge

  15. CMV+ (mScarlet-I) plasmid with onboard mCitrine cassette

    Plasmid
    #169743
    Purpose
    CMV+ circuit plasmid that encodes mScarlet-I to report the circuit output levels. This plasmid also encodes a separate mCitrine expression cassette to monitor plasmid dosage.
    Depositor
    Francois St-Pierre
    Article
    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:
    Insert
    mScarlet-I
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pCMV-tetO2
    Available since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    169743-plasmid-map-sequence-id-334355

    Enlarge

  16. pCMV-T7-SpCas9-VRER-P2A-mScarlet (MNW003)

    Plasmid
    #174138
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRER(D1135V/G1218R/R1335E/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9-VRER with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    VRER=D1135V/G1218R/R1335E/T1337R
    Promoter
    CMV and T7
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174138-plasmid-map-sequence-id-341915

    Enlarge

  17. pCMV-T7-SpCas9-VRQR-P2A-mScarlet (MNW004)

    Plasmid
    #174139
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpCas9-VRQR(D1135V/G1218R/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpCas9-VRQR with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    VRQR=D1135V/G1218R/R1335Q/T1337R
    Promoter
    CMV and T7
    Available since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174139-plasmid-map-sequence-id-341913

    Enlarge

  18. Equalizer-L (mScarlet-I) plasmid with onboard mCitrine cassette

    Plasmid
    #169737
    Purpose
    Equalizer-L plasmid that encodes mScarlet-I to report the circuit output levels. This plasmid also encodes a separate mCitrine expression cassette to monitor plasmid dosage.
    Depositor
    Francois St-Pierre
    Article
    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:
    Insert
    miR(FF4) target-tetR-P2A-mScarletI-miR(FF4) target-miR(FF4)
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    pCMV-tetO2
    Available since
    June 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    169737-plasmid-map-sequence-id-334143

    Enlarge

Showing: 761 - 780 of 6305 results