Addgene

We narrowed to 2,130 results for: CO

Showing: 761 - 780 of 2130 results

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pcDNA3 merlin 1-595

Plasmid

#11623

Depositor

Vijaya Ramesh

Article

Xu et al Exp Cell Res. 1998 Jan 10. 238(1):231-40

Insert

merlin (NF2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

May 3, 2006

Availability

Academic Institutions and Nonprofits only
pX330_ATP1A1_G7_Dual_sgRNA

Plasmid

#173206

Purpose

Coselection for HDR in human cells. Vector for dual expression of ATP1A1 G7 sgRNA in combination with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1 G7 sgRNA + user-specified sgRNA + SpCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Dual U6 promoters

Available since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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PCXLE-hUL + mTAGBFP2

Plasmid

#74944

Purpose

Dosage control and tracing of reprogramming episomes

Depositor

Ann Zovein

Article

Schmitt et al Stem Cell Res Ther. 2017 Jun 5;8(1):132.

Insert

LMyc/Lin28 (MYCL, LIN28A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pWzyE-distrepII

Plasmid

#196667

Purpose

Expression of C-terminally distrep tag II-tagged WzyE from Pectobacterium atrosepticum in E.coli

Depositor

James Naismith

Article

Weckener et al Open Biol. 2023 Mar;13(3):220373. doi: 1

Insert

WzyE

Use

Tags

TEV-cleavable di-strep tag II

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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p2xFLAGhYAP1-WW mutant

Plasmid

#17792

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

Yes-kinase associated protein (YAP1 Human)

Use

Tags

2xFlag

Expression

Mammalian

Mutation

Tryptophan 199 to Alanine; Proline 202 to Alanine

Promoter

Available since

May 9, 2008

Availability

Academic Institutions and Nonprofits only

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pET28a-LgBiT-SpyCatcher

Plasmid

#223634

Purpose

Protein purification of SpyCatcher003 (S49C) with LgBiT and 6xHis tags from E. coli

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

SpyCatcher003 S49C

Use

Tags

His6, LgBiT

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3 ss-FLAG-AP-hNDP (aa 25-133)

Plasmid

#115789

Purpose

Expresses and secretes human Norrin with N-terminal flag and alkaline phosphatase tags, contains artificial signal sequence upstream of tags.

Depositor

Harald Junge

Article

Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

Insert

NDP (NDP Human)

Use

Tags

alkaline phosphatase and flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pFB-GTE-COI1

Plasmid

#29516

Depositor

Ning Zheng

Article

Sheard et al Nature. 2010 Nov 18. 468(7322):400-5.

Insert

COI1 (COI1 Mustard Weed)

Use

Tags

GST and TEV cleavage site

Expression

Insect

Mutation

Note that the first AA in TEV site is R, not E

Promoter

Available since

May 26, 2011

Availability

Academic Institutions and Nonprofits only
p2xFLAGhYAP2-S127A

Plasmid

#17794

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

Yes-kinase associated protein (YAP1 Human)

Use

Tags

2xFlag

Expression

Mammalian

Mutation

Serine 127 to Alanine

Promoter

Available since

May 9, 2008

Availability

Academic Institutions and Nonprofits only
ATP1A1_T804N_hPGK1_EBFP_Donor

Plasmid

#187455

Purpose

HDR donor plasmid to introduce the T804N mutation conferring cellular resistance to ouabain and the EBFP cassette to ATP1A1 intron 17.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1-T804N EBFP donor (ATP1A1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

hPGK1

Available since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-eBE-S3

Plasmid

#101093

Purpose

Expresses eBE-S3 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Cell Res. 2017 Aug 29. doi: 10.1038/cr.2

Insert

eBE-S3

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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ATP1A1_G6_T804N_Dual_pegRNA

Plasmid

#173200

Purpose

Coselection for prime editing in human cells. Vector for tandem expression of ATP1A1 T804N-G6 pegRNA with a user-specified pegRNA from two independent U6 promoters. pU6-pegRNA-GG-acceptor-like plasmid

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1 G6 T804N pegRNA

Use

CRISPR; Prime editing

Tags

Expression

Mammalian

Mutation

Promoter

Tandem U6 promoters

Available since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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ATP1A1_G4_Q118R_Dual_pegRNA

Plasmid

#173199

Purpose

Coselection for prime editing in human cells. Vector for tandem expression of ATP1A1 Q118R-G4 pegRNA with a user-specified pegRNA from two independent U6 promoters. pU6-pegRNA-GG-acceptor-like plasmid

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1 G4 Q118R pegRNA

Use

CRISPR; Prime editing

Tags

Expression

Mammalian

Mutation

Promoter

Tandem U6 promoters

Available since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pCX-2A-2A

Plasmid

#74670

Purpose

based on pCX-C1 with F2A1/F2A2 inserted

Depositor

Roberto Giovannoni

Article

De Giorgi et al Plasmid. 2015 May;79:22-9. doi: 10.1016/

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCAGGS

Available since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pCXLE-hK + E2-Crimson

Plasmid

#74946

Purpose

Dosage control and tracing of reprogramming episomes

Depositor

Ann Zovein

Article

Schmitt et al Stem Cell Res Ther. 2017 Jun 5;8(1):132.

Insert

Klf4 (KLF4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 12, 2017

Availability

Academic Institutions and Nonprofits only
pGEX4T1-Rpb4/7

Plasmid

#138484

Purpose

Expresses GST-tagged Rpb4 and His-tagged Rpb7 in bacteria

Depositor

Britt Glaunsinger

Article

Castaneda et al PLoS Pathog. 2020 Sep 4;16(9):e1008843.

Inserts

Rpb4 (POLR2D Human)
Rpb7 (POLR2G Human)

Use

Tags

6xHis and GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3 merlin 1-590

Plasmid

#11624

Depositor

Vijaya Ramesh

Article

Xu et al Exp Cell Res. 1998 Jan 10. 238(1):231-40

Insert

merlin (NF2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

May 3, 2006

Availability

Academic Institutions and Nonprofits only
pDEST47-ARL5A-GFP

Plasmid

#67399

Purpose

Mammalian expression of hArl5 with C-term GFP tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL5A (ARL5A Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

bp 70 C to T; silent mutation

Promoter

CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3 merlin 1-332

Plasmid

#11625

Depositor

Vijaya Ramesh

Article

Xu et al Exp Cell Res. 1998 Jan 10. 238(1):231-40

Insert

merlin (NF2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Amino-terminal half of merlin.

Promoter

Available since

May 3, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3 merlin 308-595

Plasmid

#11628

Depositor

Vijaya Ramesh

Article

Xu et al Exp Cell Res. 1998 Jan 10. 238(1):231-40

Insert

merlin (NF2 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Carboxy-terminal half of merlin isoform 1.

Promoter

Available since

May 3, 2006

Availability

Academic Institutions and Nonprofits only