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Plasmid#39530DepositorInsertAkt (Akt1 Mouse)
UseTagsERExpressionMammalianMutationPromoterAvailable sinceNov. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB2-SA(2)-T2A-LexA::GADfluw-Hsp70
Plasmid#78307PurposeCreating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 2DepositorInsertT2A-nlsLexA::GADflluw
UseTagsExpressionMutationPromoterAvailable sinceJuly 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSK260
Plasmid#129252Purposedual yTRAP, NM fusion to aTF1 reporting on [PSI+] state with mKate2 reporterDepositorInsertSup35 NM yTRAP sensor reporting on mKate2
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1a-V5-Vrk2
Plasmid#120240PurposeExpresses V5-tagged Vrk2 in lentiviral vectorDepositorInsertVrk2 (Vrk2 Mouse)
UseLentiviralTagsV5ExpressionMutationPromoterEF1aAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCS2-hFAM 3B-His
Plasmid#172192PurposeCoding sequence of human FAM3B pCS2 C terminal 6xHis tag used for protein purification from conditioned mediumDepositorInserthFAM 3B
UseTagsHisExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
Gata3-MIEG-hCD4
Plasmid#40568DepositorInsertGata3 (Gata3 Mouse)
UseRetroviralTagsIRES hCD4ExpressionMutationPromoterAvailable sinceMarch 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSIN-Arkadia-gRNA1
Plasmid#180374Purposetargeting mouse Arkadia geneDepositorInsertArkadia targeting gRNA (Rnf111 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromoterhuman U6Available sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
-962 human cyclin D1 promoter TCF (1) site mutant pGL3Basic
Plasmid#32733DepositorInsertCCND1 promoter (CCND1 Human)
UseLuciferaseTagsExpressionMutation-75 TCF(1) site CTTTGAT deletionPromoter-962 CCND1Available sinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pIS1 IMP-1 long UTR
Plasmid#21640DepositorInsertIGF-II mRNA-binding protein 1 UTR (IGF2BP1 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
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pDIV490
Plasmid#177698PurposePlasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.DepositorInsertsCas9
Nat
UseCRISPRTagsSV40ExpressionBacterial and YeastMutationPromoterRHR2p (Debaryomyces hansenii ) and TDH3p (Candida…Available sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 CHICA (Nigg HS342)
Plasmid#41153DepositorInsertCHICA (FAM83D Human)
UseTags3xMyc and PreScission SiteExpressionMammalianMutationV11LPromoterCMVAvailable sinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pETM33_SIN3A_PAH
Plasmid#178486PurposeBacterial expression of human domain with His-tag and GST tagDepositorInsertSIN3A_PAH (SIN3A Human, Synthetic)
UseTagsHis-GSTExpressionBacterialMutationPromoterT7/LacOAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCI-syn-fRGECO2
Plasmid#125244PurposeExpresses red fluorescent calcium sensor in hippocampal slicesDepositorInsertfRGECO2
UseTagsExpressionMammalianMutationW44Y, D432APromotersynapsinAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEF1α-owl-monkey-retroCHMP3(148)-HA
Plasmid#154252Purposeexpresses prematurely truncated owl monkey retroCHMP3 in mammalian cellsDepositorInsertretroCHMP3
UseTagsHAExpressionMammalianMutationC-terminal truncation of retroCHMP3 at amino acid…PromoterEF1-alphaAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHMWGFPR
Plasmid#64396PurposeHigh molecular weight glutenin gene promoter driving GFP expressionDepositorInsertHigh molecular weight glutenin gene promoter
UseTagsGFPExpressionPlantMutationPromoterHigh molecular weight glutenin gene promoterAvailable sinceJune 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSK247
Plasmid#131148Purposedual yTRAP, Gateway destination vector of aTF2 fusion reporting on mNeonGreenDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterpSUP35, min pCYC1Available sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1‐Afu‐rnhBΔPIP
Plasmid#108696PurposeFor expression in E. coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII with C-terminal PIP box deletionDepositorInsertrnhB
UseTagsGSTExpressionBacterialMutationC-terminal truncation (7aa) removing PIP boxPromotertacAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1‐Afu‐rnhB D101N
Plasmid#108695PurposeFor expression in E. coli, and affinity purification of N-terminally GST-tagged Archaeoglobus fulgidus RNase HII with D101N catalytic site mutationDepositorInsertrnhB
UseTagsGSTExpressionBacterialMutationD101N catalytic site mutationPromotertacAvailable sinceApril 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTC-CMV-Tet-YAP
Plasmid#85579PurposeSleeping beauty transposon for hydrodynamic tail vein injection for CreER expression and tetracyclin-dependent YAP expression (CMV promoter ) in mouse liverDepositorInsertsCreER
YAP
UseCre/LoxTagsFlag (2x)ExpressionMammalianMutationPromoterTREAvailable sinceJan. 9, 2017AvailabilityAcademic Institutions and Nonprofits only