Addgene

We narrowed to 32,731 results for: sta

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HNF4A_P2-285/-136

Plasmid

#31051

Depositor

Gerhart Ryffel

Article

Wirsing et al Diabet Med. 2010 Jun . 27(6):631-5.

Insert

HNF4A P2 promoter (HNF4A Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

-285 to -1 of P2 HNF4A promoter with mutation -13…

Promoter

Available since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only

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pET15b-ITPA_P32T

Plasmid

#60825

Purpose

to purify human ITPA from bacteria

Depositor

Youri Pavlov

Article

Stepchenkova et al J Mol Biol. 2009 Sep 25;392(3):602-13. d

Insert

ITPA (ITPA Human)

Use

Tags

His

Expression

Bacterial

Mutation

P32T

Promoter

T7

Available since

March 9, 2015

Availability

Academic Institutions and Nonprofits only
pCMV-Myr-HA-mIFITM3-C71,72,105A-CLVL

Plasmid

#58414

Purpose

Expresses murine IFITM3-C71,72,105A with an N-terminal myristoylation sequence from HIV Gag followed by an HA tag, as well as a C-terminal CaaX prenylation motif, in mammalian cells

Depositor

Howard Hang, Jacob Yount

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Use

Tags

HA internal

Expression

Mammalian

Mutation

Cysteines 71, 72, and 105 to Alanine; The sequenc…

Promoter

CMV IE

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only
HLH Bmal Pas 1-465-VenC I317D

Plasmid

#47367

Purpose

Bmal fragment cloned with Venus tag used for mutation

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Use

Tags

Venus aa 156-239

Expression

Mammalian

Mutation

aa 1-465, I317D

Promoter

CMV

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
CMV-FFluc-GPD1L-3'UTR WT

Plasmid

#32488

Depositor

Pere Puigserver

Article

Kelly et al Mol Cell Biol. 2011 Jul;31(13):2696-706.

Insert

GPD1L 3'UTR (Gpd1l Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only
pIS1-mutant RhoA 3'UTR

Plasmid

#26090

Depositor

Bob Weinberg

Article

Valastyan et al Cell. 2009 Jun 12. 137(6):1032-46.

Insert

mutant RhoA 3'UTR (RHOA Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Both miR-31 binding sites in 3’ UTR mutagenized. …

Promoter

Available since

Oct. 25, 2010

Availability

Academic Institutions and Nonprofits only
RalA (G23V)( full )-pcw107

Plasmid

#64644

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

Kris Wood, David Sabatini

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

RALA (RALA Human)

Use

Lentiviral

Tags

Expression

Mutation

G23V

Promoter

PGK

Available since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only
pLentiX2-PURO-shDIXDC1-Ms-57

Plasmid

#61232

Purpose

Lentivirus expressing shRNA to mouse DIXDC1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

Dixdc1 (Dixdc1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
pCS252

Plasmid

#33207

Depositor

David Bartel

Article

Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

Insert

MAP4K4 3'UTR (MAP4K4 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

miR-CGCG sites: aaCgCga, aaCgCgaa

Promoter

SV40 early enhancer/promoter

Available since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
CMV-FFluc-GPD1L-3'UTR Seed Mut

Plasmid

#32490

Depositor

Pere Puigserver

Article

Kelly et al Mol Cell Biol. 2011 Jul;31(13):2696-706.

Insert

GPD1L 3'UTR (Gpd1l Mouse)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 2, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3-HA p21 K163R

Plasmid

#78787

Purpose

To overexpress p21 K163R in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al Cell Death Differ. 2013 Apr;20(4):620-9.

Insert

p21 (CDKN1A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

K163R_mutation

Promoter

CMV

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA p21 K161R

Plasmid

#78788

Purpose

To overexpress p21 K161R in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al Cell Death Differ. 2013 Apr;20(4):620-9.

Insert

p21 (CDKN1A Human)

Use

Tags

HA

Expression

Mammalian

Mutation

K161R_mutation

Promoter

CMV

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1 mSesn1 (1189)

Plasmid

#66917

Purpose

bacterial expression of mouse Sesn1

Depositor

Jun Hee Lee, Michael Karin

Article

Lee et al Cell Metab. 2012 Sep 5;16(3):311-21. doi

Insert

Sesn1 (Sesn1 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pCS3-MT-BX-6MYC p21 1-89

Plasmid

#78784

Purpose

To overexpress p21 1-89 in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al Cell Death Differ. 2013 Apr;20(4):620-9.

Insert

p21 (CDKN1A Human)

Use

Tags

6xMYC

Expression

Mammalian

Mutation

Jan-89

Promoter

CMV IE94

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only
mGDF8 donor CFP-hDF

Plasmid

#72590

Purpose

Plasmid contains left and right homology arms for mouse GDF8 flanking the mCherry-puromycin-2A-ECFP-dysferlin cassette.

Depositor

Renzhi Han

Article

Xu et al Mol Ther Nucleic Acids. 2013 Jul 30;2:e1

Insert

dysferlin (DYSF Human)

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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hGDF8 donor CFP-hDF

Plasmid

#72591

Purpose

Plasmid contains left and right homology arms for human GDF8 flanking the mCherry-puromycin-2A-ECFP-dysferlin cassette.

Depositor

Renzhi Han

Article

Xu et al Mol Ther Nucleic Acids. 2013 Jul 30;2:e1

Insert

dysferlin (DYSF Human)

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 15, 2016

Availability

Academic Institutions and Nonprofits only

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Lrrc4c-AP-His

Plasmid

#71959

Purpose

Expresses the extracellular region of the LRRC4C protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Lrrc4c (Lrrc4c Mouse)

Use

Tags

AP-His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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pQCXIB-FLAG-hDIXDC1-L-S592A

Plasmid

#61225

Purpose

Retrovirus expressing long isoform of human DIXDC1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

human DIXDC1 (DIXDC1 Human)

Use

Retroviral

Tags

FLAG

Expression

Mutation

Changed Serine 592 to Alanine

Promoter

Available since

March 31, 2015

Availability

Academic Institutions and Nonprofits only
pENTR-TER+-shDIXDC1-Ms-58

Plasmid

#61236

Purpose

Entry clone encoding shRNA to mouse DIXDC1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

Dixdc1 (Dixdc1 Mouse)

Use

Entr clone

Tags

Expression

Mutation

Promoter

H1/TO

Available since

March 20, 2015

Availability

Academic Institutions and Nonprofits only
pENTR-TER+-shDIXDC1-Ms-57

Plasmid

#61235

Purpose

Entry clone encoding shRNA to mouse DIXDC1

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

Dixdc1 (Dixdc1 Mouse)

Use

Entr clone

Tags

Expression

Mutation

Promoter

H1/TO

Available since

March 20, 2015

Availability

Academic Institutions and Nonprofits only