Addgene

We narrowed to 8,097 results for: sgRNA

Showing: 7761 - 7780 of 8097 results

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NFATc1-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75239

Purpose

CRISPR/Cas9 NICKASE plasmid against human NFATc1 (2/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc1 (NFATC1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgMap3K12(1)-U6-sgMap3K12(2)-hSyn1-mCherry

Plasmid

#208835

Purpose

Knockout of Map3K12 (DLK) using tandem sgRNAs, expresses mCherry under the hSyn1 promoter

Depositor

Ben Emery

Article

Duncan et al Nat Commun. 2024 Oct 23;15(1):9148. doi:

Insert

mCherry

Use

AAV and CRISPR

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

hSyn1

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgMap3K13(1)-U6-sgMap3K13(2)-hSyn1-mCherry

Plasmid

#208836

Purpose

Knockout of Map3K13 (LZK) using tandem sgRNAs, expresses mCherry under the hSyn1 promoter

Depositor

Ben Emery

Article

Duncan et al Nat Commun. 2024 Oct 23;15(1):9148. doi:

Insert

mCherry

Use

AAV and CRISPR

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

hSyn1

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgMap3K13(1)-U6-sgMap3K12(1)-hSyn1-mCherry

Plasmid

#208837

Purpose

Knockout of Map3K12 (DLK) and Map3K13 (LZK) using tandem sgRNAs, expresses mCherry under the hSyn1 promoter

Depositor

Ben Emery

Article

Duncan et al Nat Commun. 2024 Oct 23;15(1):9148. doi:

Insert

mCherry

Use

AAV and CRISPR

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

hSyn1

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-sgRev-erba-hSyn-mCherry-KASH

Plasmid

#223226

Purpose

guideRNA targeting the mouse Rev-erb alpha (Nr1d1)

Depositor

Kyungjin Kim

Article

Park et al Commun Biol. 2024 Aug 15;7(1):998. doi:

Insert

Nr1d1 (Nr1d1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-sgPet-1-hSyn-mCherry-KASH

Plasmid

#223227

Purpose

guideRNA targeting the mouse Pet-1 (Fev)

Depositor

Kyungjin Kim

Article

Park et al Commun Biol. 2024 Aug 15;7(1):998. doi:

Insert

Fev (Fev Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pHIV(T9)-DERA-sgresis

Plasmid

#222622

Purpose

Lentiviral vector that expresses sgRNA resistant DERA in mammalian cells

Depositor

Daniel Durocher

Article

Zhao et al Mol Cell. 2023 Aug 3;83(15):2792-2809.e9

Insert

DERA (DERA Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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AAV-haA3A-CBE-G-C

Plasmid

#220899

Purpose

AAV genome encoding the C-terminal of haA3A-CBE-G, and sgRNA

Depositor

Dali Li

Article

Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

Insert

haA3A-CBE-G-C

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD delta Loop1 and Loop2

Plasmid

#169833

Purpose

Expresses C-terminal flag-tagged CAD with deletion of amino acids R1398 - S1407 and F1366 - P1376 in allosteric domain

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

Deletions of amino acids R1398 - S1407 and F1366 …

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD delta Allosteric Domain

Plasmid

#169835

Purpose

Expresses C-terminal flag-tagged CAD with substitution of allosteric domain F1308 - C1455 with a (GGGS)X3 linker

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

Substitution of amino acids F1308 - C1455 with a …

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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PX458-3xHA-dFnCas9

Plasmid

#201954

Purpose

Mammalian expression plasmid of dead FnCas9 with T2A-EGFP and cloning backbone for sgRNA

Depositor

Debojyoti Chakraborty

Article

Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

Insert

3xHA-NLS-dFnCas9-T2A-EGFP

Use

CRISPR

Tags

3xHA, NLS, and T2A-EGFP

Expression

Mammalian

Mutation

D11A and H969A on FnCas9

Promoter

Cbh

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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PX458-3xHA-dSpCas9

Plasmid

#201953

Purpose

Mammalian expression plasmid of dead SpCas9 with T2A-EGFP and cloning backbone for sgRNA

Depositor

Debojyoti Chakraborty

Article

Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

Insert

3xHA-NLS-dSpCas9-T2A-EGFP

Use

CRISPR

Tags

3xHA, NLS, and T2A-EGFP

Expression

Mammalian

Mutation

D10A and H840A on SpCas9

Promoter

Cbh

Available since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(POU5F1_g1)-PGKpuroBFP-W

Plasmid

#211980

Purpose

Express gRNA against POU5F1 with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting POU5F1 (POU5F1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Human U6 promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(POU5F1_g15-14)-PGKpuroBFP-W

Plasmid

#211981

Purpose

Express gRNA against POU5F1 with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting POU5F1 (POU5F1 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Human U6 promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(LIN28A_5-4)-PGKpuroBFP-W

Plasmid

#211969

Purpose

Express gRNA against LIN28A with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting LIN28A (LIN28A Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Human U6 promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(MYC_2)-PGKpuroBFP-W

Plasmid

#211970

Purpose

Express gRNA against MYC with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting MYC (MYC Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Human U6 promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(MYC_7)-PGKpuroBFP-W

Plasmid

#211971

Purpose

Express gRNA against MYC with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting MYC (MYC Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Human U6 promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(ETS2_5-5)-PGKpuroBFP-W

Plasmid

#211959

Purpose

Express gRNA against ETS2 with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting ETS2 (ETS2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Human U6 promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(ETV4_5-1)-PGKpuroBFP-W

Plasmid

#211960

Purpose

Express gRNA against ETV4 with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting ETV4 (ETV4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Human U6 promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pKLV2-U6gRNA5(ETV4_5-2)-PGKpuroBFP-W

Plasmid

#211961

Purpose

Express gRNA against ETV4 with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting ETV4 (ETV4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Human U6 promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 7761 - 7780 of 8097 results

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