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Addgene
Showing: 7761 - 7780 of 14137 results

  1. pPL6873_2xcHS4::mCherry::2xcHS4_FRT-PuroR-P2A-mTagBFP2-NLS

    Plasmid
    #217970
    Purpose
    Provides expression from a CMV promoter surrounded by insulators. Integrates at FRT site. Confers puromycin resistance. Expresses nuclear localized BFP.
    Depositor
    Robert Piper
    Insert
    2xcHS4::mCherry::2xcHS4_FRT-PuroR-P2A-mTagBFP2-NLS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    217970-plasmid-map-sequence-id-436105

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  4. pCAG-IRES2-p75NTR-GFP

    Plasmid
    #182947
    Purpose
    To express the rat p75NTR neurotrophin receptor. The gene contains an HA tag at the N-Terminal
    Depositor
    Pantelis Tsoulfas
    Insert
    p75NTR-mGFP
    Use
    Tags
    HA tag and mGFP
    Expression
    Mammalian
    Mutation
    The leader sequence is from preprotrypin
    Promoter
    CAG
    Availability
    Academic Institutions and Nonprofits only
    182947-plasmid-map-sequence-id-360205

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  5. pAAV-CAG-2xNLS-tdTomato-WPRE-hGHpolyA

    Plasmid
    #192552
    Purpose
    Expresses nuclear localized tdTomato under CAG promoter
    Depositor
    Guoping Feng
    Has Service
    AAV PHP.eB and AAV9
    Insert
    tdTomato
    Use
    AAV
    Tags
    NLS
    Expression
    Mutation
    deleted E273 and G274 compared to FPbase ID: PGG5S
    Promoter
    CAG
    Availability
    Academic Institutions and Nonprofits only
    192552-plasmid-map-sequence-id-403193

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  6. pAAV-MP-84-spCas9-U6-gRNA

    Plasmid
    #206935
    Purpose
    Expression of spCas9 endonuclease and gRNA in an AAV vector allowing packaging of viral genome smaller than 5 kb.
    Depositor
    Markus Grompe
    Insert
    spCas9
    Use
    AAV
    Tags
    SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    MP-84
    Availability
    Academic Institutions and Nonprofits only
    206935-plasmid-map-sequence-id-407781

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  7. 5E16 (PfPKG, PF14_0346)

    Plasmid
    #79518
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PF14_0346
    Use
    Tags
    6xHis and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Availability
    Academic Institutions and Nonprofits only
    79518-plasmid-map-sequence-id-150101

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  8. pET32a-His-FADD

    Plasmid
    #78803
    Purpose
    To purify His-tagged recombinant FADD protein
    Depositor
    Jaewhan Song
    Insert
    GIG3 (FADD Human)
    Use
    Tags
    6x His, S-Tag, Thrombin cleavage site, and TrxA (…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only

  9. pVP16-tev-PRT1

    Plasmid
    #83405
    Purpose
    bacterial expression vector pVP16 containing Arabidopsis thaliana PROTEOLYSIS 1 (PRT1) N-end rule E3 ubiquitin ligase to be used in N-end rule in vitro ubiquitination studies
    Depositor
    Nico Dissmeyer
    Insert
    PROTEOLYSIS1 (PRT1) (PRT1 Mustard Weed, E. coli)
    Use
    Tags
    His8, MBP, and tev cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    83405-plasmid-map-sequence-id-175081

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  10. pCVL TAL(Nd154C63) T7 Age-Xho/Xba-Sal linkers

    Plasmid
    #50624
    Purpose
    pTAL plasmid for cloning of megaTALs or other TAL-fusions and expression in mammalian cells. RVDs are cloned between Age-Xho sites and meganuclease/fusion is cloned between Xba-Sal sites.
    Depositor
    Andrew Scharenberg
    Type
    Empty backbone
    Use
    Mrna production
    Tags
    Expression
    Mutation
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
    50624-plasmid-map-sequence-id-75688

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  11. pCVL RipTAL(Nd154C63) SFFV Xba-Sal linker

    Plasmid
    #50627
    Purpose
    pRipTAL plasmid compatible with Golden Gate TALEN cloning system. For cloning of megaTALs or other TAL-fusions and expression in mammalian cells. Meganuclease/fusion is cloned between Xba-Sal sites.
    Depositor
    Andrew Scharenberg
    Type
    Empty backbone
    Use
    Lentiviral and TALEN
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    50627-plasmid-map-sequence-id-79594

    Enlarge

  16. USP21 (3MTN)

    Plasmid
    #28112
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    USP21 (USP21 Human)
    Use
    Tags
    His tag with Thrombin cleavage
    Expression
    Bacterial
    Mutation
    catalytic domain only (amino acid residues 209-56…
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits

  17. MPP7

    Plasmid
    #28149
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    membrane protein, palmitoylated 7 (MPP7 Human)
    Use
    Tags
    His tag with thrombin cleavage
    Expression
    Bacterial
    Mutation
    PDZ Domain (residues 135-225)
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits

  18. UBH14

    Plasmid
    #28158
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    E3 ligase UHRF2 (UHRF2 Human)
    Use
    Tags
    His tag with thrombin cleavage
    Expression
    Bacterial
    Mutation
    SRA Domain (residues 419-648)
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits

  19. MPP8 (3R93)

    Plasmid
    #32050
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    MPP8 (MPHOSPH8 Human)
    Use
    Tags
    His (with TEV cleavage)
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits

  20. pTH671-SUP35-H348Q

    Plasmid
    #29732
    Depositor
    Tobias von der Haar
    Insert
    SUP35 gene encoding translation release factor 3 from S. cerevisiae (SUP35 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    H348Q mutant gene of SUP35 including genomic sequ…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    29732-plasmid-map-sequence-id-13818

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Showing: 7761 - 7780 of 14137 results