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pAAV.CAG.Ruby2sm-Flag.WPRE.SV40

Plasmid

#98925

Purpose

AAV expression of Ruby2 spaghetti monster FLAG construct

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

Ruby2-smFP-Flag

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CBA

Available since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti6.3 GCN1-3xFlag_IRES-iRFP

Plasmid

#198388

Purpose

GCN1 expression

Depositor

Jack Taunton

Article

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

GCN1 (GCN1 Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pET28 MBP_PrA/G-H6

Plasmid

#176077

Purpose

Express His6 tagged MBP_PrA/G in Escherichia coli

Depositor

Tom Rapoport

Article

Wu et al Proc Natl Acad Sci U S A. 2021 Oct 12;11

Insert

MBP PrA/G-H6

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7 Promoter

Available since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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NSUN2

Plasmid

#188059

Purpose

Bacterial expression plasmid for human NSUN2; for activity testing

Depositor

Tanja Schirmeister

Article

Schwickert et al J Med Chem. 2022 Jul 18. doi: 10.1021/ac

Insert

NSUN2 (NSUN2 Human)

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pACEBac1-GCP6

Plasmid

#178072

Purpose

Insect cell expression of GCP6

Depositor

Tarun Kapoor

Article

Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

Insert

GCP6 (TUBGCP6 Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pSLIK-Hygro

Plasmid

#25737

Purpose

3rd generation Lentiviral vector for Tet-based inducible shRNA or cDNA expression, constitutive Hygromycin resistance gene coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only

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TAAR8-Tango

Plasmid

#66512

Purpose

Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

Depositor

Bryan Roth

Article

Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

Insert

TAAR8 (TAAR8 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
tetO-FUW-eGFP-RHOA-Q63L

Plasmid

#73081

Purpose

Lentiviral transfer vector for tet-inducible expression of EGFP tagged human RHOA-Q63L

Depositor

Andrew Putnam

Article

Kong et al Sci Rep. 2013 Dec 11;3:3474. doi: 10.103

Insert

RHOA-Q63L (RHOA Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

Q63L Constitutively active

Promoter

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pSA101

Plasmid

#59787

Purpose

drives ZIF-1 protein in C. elegans neurons

Depositor

Jeremy Nance

Article

Armenti et al Development. 2014 Dec;141(23):4640-7. do

Inserts

rab-3 promoter
zif-1 coding (zif-1 Nematode)
unc-54 3' UTR

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/hChR2(H134R)-mCherry

Plasmid

#20938

Purpose

Mammalian expression of humanized ChR2 with H134R mutation fused to mCherry for optogenetic activation

Depositor

Karl Deisseroth

Article

Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

Insert

channelrhodopsin-2

Use

Tags

Expression

Mammalian

Mutation

H134R mutation in ChR2

Promoter

CMV

Available since

June 1, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Cerulean(1-10)

Plasmid

#165144

Purpose

Expresses Cerulean(1-10) in mammalian cells

Depositor

Daichi Kamiyama

Article

Tamura et al Commun Biol. 2021 Feb 26;4(1):257. doi:

Insert

Cerulean(1-10)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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pT2-HSP-GFP

Plasmid

#65488

Purpose

transposable GFP

Depositor

Guillaume Filion

Insert

GFP transposable cassette (amaxaGFP)

Use

Tags

Expression

Mutation

Promoter

hsp70

Available since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag-IDH1

Plasmid

#62906

Purpose

Expresses wildtype IDH1 in mammalian cells

Depositor

Yue Xiong

Article

Zhao et al Science. 2009 Apr 10;324(5924):261-5. do

Insert

isocitrate dehydrogenase 1 (NADP+), soluble (IDH1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

April 1, 2016

Availability

Academic Institutions and Nonprofits only

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pQE30-roGFP1-iL

Plasmid

#83313

Purpose

Inducible expression of redox sensitive GFP in bacteria

Depositor

S. James Remington

Article

Lohman et al Biochemistry. 2008 Aug 19;47(33):8678-88

Insert

roGFP1-iL

Use

Tags

6xHIS

Expression

Bacterial

Mutation

ro1(C48S/S147C/Q204C) plus X insertion, iL betwe…

Promoter

T5

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only
pNR291

Plasmid

#79555

Purpose

Output plasmid: contains 5-state register that regulates expression of GFP, RFP, and BFP. Expresses a unique fluorescent signature in each state.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Inserts

GFPmut3b
mTagBFP
mRFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pQCasTns(Ptr)-array 8

Plasmid

#175580

Purpose

Expression of CRISPR-associated transposases

Depositor

Sheng Yang

Article

Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

Insert

PtrTniQ, PtrCas5/8, PtrCas7, PtrCas6, PtrTnsABC, CRISPR(8 CRISPR array)

Use

Tags

Expression

Bacterial

Mutation

NO

Promoter

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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XNOR-gate-V2.0

Plasmid

#44454

Depositor

Drew Endy

Article

Bonnet et al Science. 2013 May 3;340(6132):599-603. d

Insert

XNOR-gate

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 13, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_SpyCatcher-6aa-sfCherry2(1-10)

Plasmid

#117484

Purpose

Expresses tandem binder (SpyCatcher002-6aa-sfCherry2(1-10)) in mammalian cells

Depositor

Bo Huang

Article

Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

Insert

SpyCatcher002-6aa-sfCherry2(1-10)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pDest-623 (attR4-attR3)

Plasmid

#161878

Purpose

Multisite Gateway (attR4-attR3) vector for expression of multiple proteins using the Bac-to-Bac insect cell system

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pN1-CMV-mCherryN-IntN-DrBphP (N23)

Plasmid

#210724

Purpose

NIR optogenetic module for conditional protein splicing of split mCherry (N-part)

Depositor

Vladislav Verkhusha

Article

Karasev et al J Mol Biol. 2023 Dec 15;435(24):168360.

Insert

N23

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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