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Plasmid#127326PurposeExpress 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with (AGAGAG)7 repeats in 5' UTRDepositorInsertnanoLuciferase
UseTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
FB039
Plasmid#119715PurposeMultipartite assembly obtained by combining FBparts FB026+FB009 into pDGB3omega1. Used for fluorescent tagging of fungi (G418 resistance + YFP), according to FungalBraid modular DNA assembly for ATMT.DepositorInsertPgpdA::YFP::TtrpC(←)-PtrpC:nptII::Ttub (→)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-ChromeT-GFP
Plasmid#123316PurposeAAV-mediated expression of ChromeT-GFP under the Syn promoter.DepositorInsertChromeT-GFP
UseAAVTagsGFPExpressionMammalianMutationChannelrhodopsin-2 (ChR2) with mutations A71S/ E9…PromoterSynAvailable sinceMay 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T-3-cytXlATL2
Plasmid#124063PurposeFragment of Xenopus laevis Atlastin (cytATL). This fragment contains the GTPase domain and the three-helix bundle, but lacks the transmembrane segments and the cytosolic tail.DepositorInsertcytXIATL2
UseTagsExpressionBacterialMutationPromotertacAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCIG1_UL136_19kDa_EE
Plasmid#74951PurposePlasmid for mammalian expression of HCMV protein UL136. Expresses only the 19kda isoform of UL136. Also expresses EGFP.DepositorInsertUL136 (UL136 Human Cytomegalovirus strain TB40/E)
UseLentiviralTagsGlu-Glu tag (EYMPME)ExpressionMammalianMutationdelta 1-127PromoterCMV, lentiviral LTRAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCIG1_UL136_23kDa_Myc
Plasmid#74949PurposePlasmid for mammalian expression of HCMV protein UL136. Expresses only the 23kda isoform of UL136. Also expresses EGFP.DepositorInsertUL136 (UL136 Human Cytomegalovirus strain TB40/E)
UseLentiviralTagsHA Tag (YPYDVPDYA)ExpressionMammalianMutationdelta 1-99 M128APromoterCMV, Lentiviral LTRAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
plko-tet-on puromycin-shMTH1
Plasmid#110918PurposeTo knock down MTH1 (Mut T Homolog 1) after addition of doxyclineDepositorInsertMTH1 (NUDT1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET15b+C3(R112C/T180C)X
Plasmid#108196PurposeExpresses P450cam-PCNA3(R112C/T180C)-PdX fusion protein in E. coliDepositorInsertP450cam-PCNA3(R112C/T180C)-PdX fusion protein
UseTagsHis6 tagExpressionBacterialMutationR112C/T180C(PCNA3); C73S/C85S (PdX)PromoterT7 promoterAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-SPA mimic WT
Plasmid#92350PurposeTo mimic SPA RNA processing and expressionDepositorInsertSNRPN exon 9, intron9, exon10, intron10 partial partial of SPA1 (SNRPN Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEF-HA-TSAd-Y89XF
Plasmid#84559Purposemammalian expression of TSAd-Y89XFDepositorInsertTSAd (SH2D2A Human)
UseTagsHAExpressionMammalianMutationY279F, Y290F, Y305FPromoterEF1aAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJOG250
Plasmid#80582Purposerecipient plasmid [multiplexing, activator]; p35S:Cas9 D10A/N863A-Hax3CT, nptIIDepositorInsertspnos:nptII-tnos
p35S:Cas9 D10A/N863A-Hax3(CT)-t35S
BsaI-ccdB_CmR-BsaI
UseCRISPRTagsExpressionPlantMutationD10APromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJOG251
Plasmid#80583Purposerecipient plasmid [multiplexing, activator]; p35S:Cas9 D10A/N863A-Hax3CT, BASTADepositorInsertspnos:PAT-tnos
p35S:Cas9 D10A/N863A-Hax3(CT)-t35S
BsaI-ccdB_CmR-BsaI
UseCRISPRTagsExpressionPlantMutationD10APromoterAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCIG1_UL136_25kDa_Myc
Plasmid#74954PurposePlasmid for mammalian expression of HCMV protein UL136. Expresses only the 25kda isoform of UL136. Also expresses EGFP.DepositorInsertUL136 (UL136 Human Cytomegalovirus strain TB40/E)
UseLentiviralTags3xFLAG (GGGGDYKDDDDKGADYKDDDDKEFDYKDDDDK)ExpressionMammalianMutationdelta 1-77, M100A M128APromoterCMV, Lentiviral LTRAvailable sinceMay 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Ywhab-3'UTR-MRE-1 MUT
Plasmid#61796PurposeLuciferase reporter containing the 3'UTR of mouse Ywhab with MRE-1 mutatedDepositorInsertmouse Ywhab 3'UTR with miR-200c MRE-1 mutated
UseLuciferaseTagsExpressionMutationmiR-200c MRE-1 mutatedPromoterAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Xbp1-3'UTR-MRE-3 MUT
Plasmid#61793PurposeLuciferase reporter containing the 3'UTR of mouse Xbp1 with MRE-1 mutatedDepositorInsertmouse Xbp1 3'UTR with miR-200c MRE-3 mutated
UseLuciferaseTagsExpressionMutationmiR-200c MRE-3 mutatedPromoterAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Smad5-3'UTR-MRE-1 MUT
Plasmid#61791PurposeLuciferase reporter containing the 3'UTR of mouse Smad5 with MRE-1 mutatedDepositorInsertmouse Smad5 3'UTR with miR-200c MRE-1 mutated
UseLuciferaseTagsExpressionMutationmiR-200c MRE-1 mutatedPromoterAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Map3k1-3'UTR-MRE-1 MUT
Plasmid#61790PurposeLuciferase reporter containing the 3'UTR of mouse Map3k1 with MRE-1 mutatedDepositorInsertmouse Map3k1 3'UTR with miR-200c MRE-1 mutated
UseLuciferaseTagsExpressionMutationmiR-200c MRE-1 mutatedPromoterAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Smad2-3'UTR-MRE-2+3 MUT
Plasmid#61789PurposeLuciferase reporter containing the 3'UTR of mouse Smad2 with MRE-2 and MRE-3 mutatedDepositorInsertmouse Smad2 3'UTR with both miR-200c MRE-2 and MRE-3 mutated
UseLuciferaseTagsExpressionMutationboth miR-200c MRE-2 and MRE-3 mutatedPromoterAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-2-Crtap-3'UTR-MRE-1 MUT
Plasmid#61786PurposeLuciferase reporter containing the 3'UTR of mouse Crtap with MRE-1 mutatedDepositorInsertmouse Crtap 3'UTR with miR-200c MRE-1 mutated
UseLuciferaseTagsExpressionMutationmiR-200c MRE-1 mutatedPromoterAvailable sinceApril 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
CFP(159-238) in pcDNAI/Amp
Plasmid#60896PurposeThis vector attaches CFP(159-238) to the N-terminus of a protein.DepositorInsertCFP(159-238)
UseTagsExpressionMammalianMutationAsn-164 to HisPromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only