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Addgene
Showing: 7721 - 7740 of 14137 results
  1. PRPF4.001.169.S34E

    Plasmid
    #137643
    Purpose
    Expresses N-terminal (His)6-TEV cleavage site fusion of human gene or portion of gene, with point mutation, in bacterial strains. pET based vector.
    Depositor
    Insert
    PRPF4 (PRPF4 Human)
    Use
    Tags
    (His)6-TEV
    Expression
    Bacterial
    Mutation
    001-169-S34E
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  2. PRPF4.211.522.gst

    Plasmid
    #137650
    Purpose
    Expresses N-terminal GST-(His)6-Thrombin cleavage site fusion of human gene or portion of gene in bacterial strains. pET based vector.
    Depositor
    Insert
    PRPF4 (PRPF4 Human)
    Use
    Tags
    GST-(His)6-thrombin
    Expression
    Bacterial
    Mutation
    211-522-gst
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  3. KE-005

    Plasmid
    #133440
    Purpose
    Human elF4A1 residues 1-406, N-term His tag, thrombin cleavage, in pET21 vector backbone.
    Depositor
    Insert
    EIF4A1 (EIF4A1 Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  4. pmSod1-Q42L;Q123A-N’His

    Plasmid
    #117710
    Purpose
    mutation on stability/solubility of SOD1
    Depositor
    Insert
    Sod1 (Sod1 Mouse)
    Use
    Tags
    His tag with Thrombin cleavage site
    Expression
    Bacterial
    Mutation
    Q42L; Q123A
    Promoter
    T7
    Availability
    Academic Institutions and Nonprofits only
  5. pcDNA-mTRAF3ip3-CFP

    Plasmid
    #125181
    Purpose
    Expresses mouse TRAF3IP3 in mammalian cells
    Depositor
    Insert
    TRAF3ip3 (Traf3ip3 Mouse)
    Use
    Tags
    CFP
    Expression
    Mammalian
    Mutation
    K87R, M283I, S359P, A506G- please see depositor c…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
  6. pSBbi-Pur-Lyn-AktAR2-EV

    Plasmid
    #125199
    Purpose
    SB-transposon plasmid for stable expression of lipid raft-targeted Lyn-AktAR2-EV variant of FRET-based AKT activity reporter
    Depositor
    Insert
    Lyn-AktAR2-EV
    Use
    Transposon
    Tags
    EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a/RPBSA
    Availability
    Academic Institutions and Nonprofits only
  7. pAc5.1B-EGFP-DmNot3-dsRNAres_Q

    Plasmid
    #147260
    Purpose
    Insect Expression of DmNot3-dsRNAres
    Depositor
    Insert
    DmNot3-dsRNAres (Not3 Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    one silent mutation compared to the sequence give…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
  8. A-PRMT3-3

    Plasmid
    #166514
    Purpose
    scFv of a human scaffold targeting Protein arginine methyltransferase 3 isoform 1. Antigen coverage aa 211-531 of 531.
    Depositor
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Availability
    Academic Institutions and Nonprofits only
  9. C-BRD1-3

    Plasmid
    #166517
    Purpose
    scFv of a human scaffold targeting Bromodomain-containing protein 1. Antigen coverage aa 925-1049 of 1058, PWWP domain.
    Depositor
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Availability
    Academic Institutions and Nonprofits only
  10. G-BRD4-4

    Plasmid
    #166518
    Purpose
    scFv of a human scaffold targeting Bromodomain-containing protein 4 isoform long. Antigen coverage aa 44-168 of 1362, Bromodomain 1.
    Depositor
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Availability
    Academic Institutions and Nonprofits only
  11. J-SPRTNC-1

    Plasmid
    #166521
    Purpose
    scFv of a human scaffold targeting SprT-like N-terminal domain. Antigen coverage aa 1-216 of 489
    Depositor
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Availability
    Academic Institutions and Nonprofits only
  12. J-GALTA-3

    Plasmid
    #166523
    Purpose
    scFv of a human scaffold targeting Galactose-1-phosphate uridylyltransferase. Antigen coverage aa 1-379 of 379, full-length
    Depositor
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Availability
    Academic Institutions and Nonprofits only
  13. J-DHTKD1A-1

    Plasmid
    #166524
    Purpose
    scFv of a human scaffold targeting Dehydrogenase E1 and transketolase domain containing protein 1. Antigen coverage aa 45-919 of 919
    Depositor
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Availability
    Academic Institutions and Nonprofits only
  14. J-IRF5-5

    Plasmid
    #166525
    Purpose
    scFv of a human scaffold targeting Interferon regulatory factor 5. Antigen coverage aa E232-L434 of 498
    Depositor
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Availability
    Academic Institutions and Nonprofits only
  15. L-SACSIN-74

    Plasmid
    #166526
    Purpose
    scFv of a human scaffold targeting Sacsin, DnaJ homolog subfamily C member 29. Antigen coverage aa 84-339 of 4579
    Depositor
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, Sortag, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Availability
    Academic Institutions and Nonprofits only
  16. L-YARS-18

    Plasmid
    #166534
    Purpose
    scFv of a human scaffold targeting Tyrosyl-tRNA synthetase. Antigen coverage aa 1-528 of 528, full-length
    Depositor
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Availability
    Academic Institutions and Nonprofits only
  17. O-AASSA-25

    Plasmid
    #166537
    Purpose
    scFv of a human scaffold targeting Aminoadipate-semialdehyde synthase. Antigen coverage aa 455-926 of 926
    Depositor
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Availability
    Academic Institutions and Nonprofits only
  18. O-FHL1-1

    Plasmid
    #166543
    Purpose
    scFv of a human scaffold targeting Four and a half LIM domains protein 1. Antigen coverage aa 1-280 aa, full-length
    Depositor
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Availability
    Academic Institutions and Nonprofits only
Showing: 7721 - 7740 of 14137 results