Addgene

We narrowed to 28,063 results for: eng

Showing: 7701 - 7720 of 28063 results

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pBC2103-reci

Plasmid

#117172

Purpose

U6-sgRNA-EFS-arC9-T2A-mCherry-P2A-Hygro; arC9 without NLS

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

reci_arCas9 without NLS

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pP2-phiV10

Plasmid

#196333

Purpose

Plasmid expressing chimeric P2-phiV10 tail fiber

Depositor

Baojun Wang

Article

Fa-Arun et al ACS Synth Biol. 2023 Feb 17;12(2):596-60

Insert

chimeric tail fiber P2-phiV10

Use

Tags

Expression

Bacterial

Mutation

Promoter

P2 late promoter

Available since

June 6, 2023

Availability

Academic Institutions and Nonprofits only

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pAC-Kan-alphaGal4

Plasmid

#13421

Depositor

Keith Joung

Article

Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

Insert

RNAP alpha-Gal4

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 8, 2007

Availability

Academic Institutions and Nonprofits only

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pKTrfp

Plasmid

#59772

Purpose

pKT230 derivative; PBAD_rfp; cmR

Depositor

Nathan Hillson

Article

Bi et al Microb Cell Fact. 2013 Nov 13;12:107. do

Insert

rfp

Use

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Sept. 26, 2014

Availability

Academic Institutions and Nonprofits only

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LP538

Plasmid

#90202

Purpose

pCMV-myc with cDNA encoding HsTRAPPC8 full length

Depositor

Lotte Pedersen

Article

Verdier et al Methods Mol Biol. 2016;1454:15-33. doi:

Insert

TRAPPC8 (TRAPPC8 Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

full length protein, no mutations

Promoter

CMV

Available since

June 2, 2017

Availability

Academic Institutions and Nonprofits only

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pmCherry-C1-p63RhoGEF

Plasmid

#67896

Purpose

Expresses a fusion of mCherry with full-length p63RhoGEF in mammalian cells

Depositor

Dorus Gadella

Article

van_Unen et al Scientific Reports 5, Article number: 14

Insert

mCherry-p63RhoGEF

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pBT224_(pCA-tTA2)

Plasmid

#36430

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Insert

tetracycline transactivator

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG (chicken beta actin promoter and CMV enhancer)

Available since

June 25, 2012

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 PC

Plasmid

#67132

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

PC (PC Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pMMB67EH-YFP

Plasmid

#90104

Purpose

YFP control expression plasmid

Depositor

Samuel Miller

Article

Christen et al Science. 2010 Jun 4;328(5983):1295-7. do

Insert

YFP

Use

Broad-host range

Tags

Expression

Bacterial

Mutation

Promoter

Ptac

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pNC-121-35S

Plasmid

#193397

Purpose

Nimble cloning-compatible vector for plant ectopic expression

Depositor

Pu Yan

Article

Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQ132-tRNA

Plasmid

#179211

Purpose

Golden Gate entry vector; 2nd gRNA with tRNA

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2022 May;8(5):513-525. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

no promoter

Available since

May 23, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_Lifeact_HaloTag7

Plasmid

#175535

Purpose

Expression of HaloTag7 at the actin filaments of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Lifeact-HaloTag7

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pHR_EF1a-->myc-mtLaG2

Plasmid

#162224

Purpose

Lentiviral vector for constitutive expression of Myc GFP nanobody Lag2 in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

anti-GFP nanobody LaG2

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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DV4 VLPs pVRC8400

Plasmid

#63164

Purpose

DV4 prM-E structural cassette in pVRC8400

Depositor

Stephen Harrison

Article

Chao et al Elife. 2018 Jul 12;7. pii: 36461. doi: 1

Insert

DV4 prM-E

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pSB1C3-J23110-B0034-mRFP1_Pink

Plasmid

#160445

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Pink chromoprotein in E. coli

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

promoter, RBS, mRFP1E_Pink

Use

Synthetic Biology

Tags

Expression

Mutation

BioBrick sites removed

Promoter

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pPdR

Plasmid

#85083

Purpose

Expresses putidaredoxin reductase in E. coli

Depositor

Teruyuki Nagamune

Article

Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

Insert

Putidaredoxin reductase

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB1-2-PT-SA-SD-0-2xTY1-V5

Plasmid

#61255

Purpose

CRISPR-RMCE, second step attB plasmid, intron phase 0, exchange dsRed with 2xTY1 and V5 tags cassette

Depositor

Frank Schnorrer

Article

Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

Insert

2xTY1-V5

Use

CRISPR

Tags

TY1 and V5

Expression

Mutation

Promoter

Available since

Nov. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_LAMP1_HaloTag7

Plasmid

#175531

Purpose

Lyzosomal expression of HaloTag7 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

LAMP1-HaloTag7

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pSB1C3-J23110-B0034-mRFP1_Orange

Plasmid

#160444

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Orange chromoprotein in E. coli

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

promoter, RBS, mRFP1E_Orange

Use

Synthetic Biology

Tags

Expression

Mutation

BioBrick sites removed

Promoter

Available since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pBsaBM3

Plasmid

#85102

Purpose

P450 BM3 variant (A74G/C773S/C810S)

Depositor

Teruyuki Nagamune

Article

Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

Insert

P450 BM3

Use

Cloning vector

Tags

Expression

Mutation

A74G/C773S/C810S

Promoter

Available since

Dec. 14, 2016

Availability

Academic Institutions and Nonprofits only

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