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Addgene
Showing: 7701 - 7720 of 14137 results

  1. PP-MAPK1 (3OZ6)

    Plasmid
    #28164
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    mitogen-activated protein kinase 1 (cgd2_1960 Cryptosporidium parvum)
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    contains residues 4-390
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits

  2. MUM1

    Plasmid
    #28273
    Depositor
    Cheryl Arrowsmith
    Insert
    MUM1 (MUM1 Human)
    Use
    Tags
    His with TEV cleavage
    Expression
    Bacterial
    Mutation
    Contains PWWP Domain (residues 405-538).
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits

  3. TDRD3 (3PMT)

    Plasmid
    #28274
    Depositor
    Cheryl Arrowsmith
    Insert
    Tudor domain-containing protein 3 (TDRD3 Human)
    Use
    Tags
    His with TEV cleavage
    Expression
    Bacterial
    Mutation
    contains Tudor domain, residues 553-611.
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits

  4. UKC1620-SUP35wt

    Plasmid
    #29730
    Depositor
    Tobias von der Haar
    Insert
    SUP35 gene encoding translation release factor 3 from S. cerevisiae (SUP35 Budding Yeast)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Wild type gene including genomic sequence 955 bp …
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  8. 4YWQ

    Plasmid
    #69953
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RC3H1:YTC019-B07:C232791 (RC3H1 Human)
    Use
    Tags
    His and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Availability
    Academic Institutions and Nonprofits only

  9. 5C7J

    Plasmid
    #69965
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    NEDD4:SDC094-F02:C29544 (NEDD4 Human)
    Use
    Tags
    His and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Availability
    Academic Institutions and Nonprofits only

  10. 5C8H

    Plasmid
    #69969
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    ORC2:JMC095-D11:C231601 (ORC2 Human)
    Use
    Tags
    His and TEV cleavage site
    Expression
    Bacterial
    Mutation
    Promoter
    T7-lacO
    Availability
    Academic Institutions and Nonprofits only

  12. pFB-CT10HF-LIC

    Plasmid
    #39191
    Purpose
    SGC Baculovirus transfer vector with C-terminal His tag and FLAG tag, preceded by a TEV protease cleavage site. Includes sites for LIC cloning, and SacB gene for negative selection
    Depositor
    Nicola Burgess-Brown
    Type
    Empty backbone
    Use
    Baculovirus
    Tags
    TEV-His-FLAG
    Expression
    Mutation
    Promoter
    polyhedrin
    Availability
    Academic Institutions and Nonprofits only
    39191-plasmid-map-sequence-id-136169

    Enlarge

  13. pSaCas9_GFP

    Plasmid
    #64709
    Purpose
    Co-expression of human codon-optimized Staphylococcus aureus Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells and other mammalian cells
    Depositor
    Kiran Musunuru
    Insert
    SaCas9-2A-GFP
    Use
    CRISPR
    Tags
    2A-GFP and 3xFLAG-SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Availability
    Academic Institutions and Nonprofits only
    64709-plasmid-map-sequence-id-322473

    Enlarge

  14. pCMV-P10HA

    Plasmid
    #1345
    Depositor
    Li-Huei Tsai
    Insert
    P10 (CDK5R1 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only

  15. Dendra2-PCNA-19-SV40NLS-4

    Plasmid
    #57735
    Purpose
    Localization: Nucleus, Excitation: 490 / 553, Emission: 507 / 573
    Depositor
    Michael Davidson
    Insert
    PCNA-19 SV40NLS (PCNA Human)
    Use
    Tags
    Dendra2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Industry, Academic Institutions, and Nonprofits

  16. JDS246

    Plasmid
    #43861
    Purpose
    Expresses mammalian codon optimized Cas9 nuclease with C-term 3X FLAG from CMV and T7 promoters
    Depositor
    Keith Joung
    Insert
    mammalian codon-optimized streptococcus pyogenes Cas9 - 3X Flag
    Use
    CRISPR
    Tags
    3X FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    43861-plasmid-map-sequence-id-59200

    Enlarge

  17. pcDNA-mTRAF3ip3-CFP

    Plasmid
    #125181
    Purpose
    Expresses mouse TRAF3IP3 in mammalian cells
    Depositor
    Shao-Cong Sun
    Insert
    TRAF3ip3 (Traf3ip3 Mouse)
    Use
    Tags
    CFP
    Expression
    Mammalian
    Mutation
    K87R, M283I, S359P, A506G- please see depositor c…
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    125181-plasmid-map-sequence-id-243305

    Enlarge

  18. pSBbi-Pur-Lyn-AktAR2-EV

    Plasmid
    #125199
    Purpose
    SB-transposon plasmid for stable expression of lipid raft-targeted Lyn-AktAR2-EV variant of FRET-based AKT activity reporter
    Depositor
    Eric Davis
    Insert
    Lyn-AktAR2-EV
    Use
    Transposon
    Tags
    EV linker, cpVenus[E172] and Lyn tag (GCIKSKRKD),…
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a/RPBSA
    Availability
    Academic Institutions and Nonprofits only
    125199-plasmid-map-sequence-id-243587

    Enlarge

  19. pAc5.1B-EGFP-DmNot3-dsRNAres_Q

    Plasmid
    #147260
    Purpose
    Insect Expression of DmNot3-dsRNAres
    Depositor
    Elisa Izaurralde
    Insert
    DmNot3-dsRNAres (Not3 Fly)
    Use
    Tags
    Expression
    Insect
    Mutation
    one silent mutation compared to the sequence give…
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    147260-plasmid-map-sequence-id-289707

    Enlarge

Showing: 7701 - 7720 of 14137 results