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Plasmid#112609PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and GB1 tag fusions and with C-terminal Avi-tagDepositorTypeEmpty backboneUseTags8His-GB1 and AviExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only
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FusionRed-FTR940-N-11
Plasmid#62737PurposeLocalization: Nuclear - Nucleolus, N1 Cloning Vector, Excitation:580, Emission:608DepositorInsertFTR940
UseTagsFusionRedExpressionMammalianMutationnonePromoterCMVAvailable sinceApril 1, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET15b (I-WcaINhis) K226I
Plasmid#195620PurposeExpresses I-WcaI homing endonuclease from Wickerhamomyces canadensis containing the K226I mutationDepositorInsertI-WcaI
UseTagsExpressionBacterialMutationA1930T, A1931TPromoterT7Available sinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET15b (I-WcaINhis) K125I
Plasmid#195619PurposeExpresses I-WcaI homing endonuclease from Wickerhamomyces canadensis containing the K125I mutationDepositorInsertI-WcaI
UseTagsExpressionBacterialMutationA2030T, A2031TPromoterT7Available sinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOP5TT
Plasmid#112613PurposeUsed for expression of protein in E. coli as TEV cleavable N-terminual 8His and thioredoxin tag fusions and with C-terminal Avi-tagDepositorTypeEmpty backboneUseTags8His-thioredoxin and AviExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOP5BP
Plasmid#112608PurposeUsed for expression of protein in E. coli as PreScission cleavable N-terminual 8His and GB1 tag fusions and with C-terminal Avi-tagDepositorTypeEmpty backboneUseTags8His-GB1 and AviExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
HA-CPI-17-wt-NLS
Plasmid#100776PurposeMammalian expression of CPI-17 nuclearDepositorInsertCPI-17-NLS (PPP1R14A Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-LIC
Plasmid#26094PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneUseTagsHexahistidine tag with Thrombin cleavageExpressionBacterialMutationPromoterAvailable sinceAug. 20, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28-MHL
Plasmid#26096PurposeSGC Empty backbone for bacterial expressionDepositorTypeEmpty backboneUseTagsHexahistidine tag with TEV cleavageExpressionBacterialMutationPromoterT7-lacOAvailable sinceAug. 20, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-EcoRV
Plasmid#165548PurposeType II restriction enzyme that recognizes the double-stranded sequence GATATC and cleaves after T-1.DepositorInsertEcoRV
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 10, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-EcoRIR
Plasmid#165504PurposeType II restriction enzyme that recognizes the double-stranded sequence GAATTC and cleaves after G-1.DepositorInsertEcoRI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-XbaIR
Plasmid#165567PurposeType II restriction enzyme that recognizes the double-stranded sequence TCTAGA and cleaves after T-1.DepositorInsertXbaI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-PstIR
Plasmid#165526PurposeRestriction enzyme that recognizes the double-stranded sequence CTGCAG and cleaves after A-5.DepositorInsertPstI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 5, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHS2
Plasmid#81037PurposeHO endonuclease expressionDepositorInsertHYGMX
UseTagsExpressionYeastMutationPromoterTEFAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOpen-KTHEAQpol
Plasmid#165542PurposeExonuclease-free, N-terminal deletion mutant of Taq DNA polymerase.DepositorInsertKlenTaq1 polymerase
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 9, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-HindIIIR
Plasmid#165508PurposeType II restriction enzyme that recognizes the double-stranded sequence AAGCTT and cleaves after A-1.DepositorInsertHindIII
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-NcoIR
Plasmid#165568PurposeType II restriction enzyme that recognizes the double-stranded sequence CCATGG and cleaves after C-1.DepositorInsertNcoI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 18, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-SapIR
Plasmid#165537PurposeType II restriction enzyme that recognizes the double-stranded sequence GCTCTTC(N) and cleaves after N-1.DepositorInsertSapI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 5, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-EcoRVM
Plasmid#165558PurposeSite-specific DNA methyltransferase; protects DNA from cleavage by EcoRV.DepositorInsertM.EcoRV
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 16, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pOpen-SapIM2
Plasmid#165557PurposeSite-specific DNA methyltransferase 2; protects DNA from cleavage by SapIDepositorInsertM2.SapI
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 16, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits