Addgene

We narrowed to 19,047 results for: tes

Showing: 7641 - 7660 of 19047 results

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pCDNA3.1(-) + SLNCR1

Plasmid

#86816

Purpose

mammalian expression of lncRNA LINC00673/SLNCR1

Depositor

Carl Novina

Article

Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

Insert

LINC00673 (LINC00673 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 2, 2017

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(2)-T2A-dVP16AD-Hsp70

Plasmid

#62911

Purpose

Creating dVP16AD hemidrivers from MiMIC lines with inserts into coding introns in Phase 2

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

T2A-dVP16AD-Hsp70

Use

Tags

Expression

Mutation

Promoter

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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ME2/pBABE-puro

Plasmid

#49164

Purpose

For expression of Malic Enzyme 2 in mammalian cells from retroviral vector

Depositor

Xiaolu Yang

Article

Jiang et al Nature. 2013 Jan 31;493(7434):689-93. do

Insert

Malic Enzyme 2 (ME2 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Nov. 15, 2013

Availability

Academic Institutions and Nonprofits only
pLV-EF1a-V5-Sec61B

Plasmid

#120244

Purpose

Expresses V5-tagged Sec61B in lentiviral vector

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Insert

Sec61B (Sec61b Mouse)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

EF1a

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pICE-EGFP-FLAG-Ku70siR-WT

Plasmid

#46961

Purpose

Plasmid for constitutive or doxycycline-inducible expression of wild-type human Ku70 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

Depositor

Steve Jackson

Article

Britton et al J Cell Biol. 2013 Jul 29.

Insert

human Ku70 (XRCC6 Human)

Use

Tags

GFP-FLAG

Expression

Mammalian

Mutation

Promoter

CMV Tet

Available since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pGL2-100bp

Plasmid

#16294

Depositor

Wafik El-Deiry

Article

Wang et al Cancer Res. 2006 Jul 15. 66(14):6982-9.

Insert

p53 promoter fragment (TP53 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

fragment -88 to +12 relative to first transcripti…

Promoter

Available since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only

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pBS-KS-attB2-SA(1)-T2A-3X Gal80-Hsp70

Plasmid

#62952

Purpose

Creating Gal80 lines from MiMIC lines with inserts into coding introns in Phase 1. Gal80 is tagged with FLAG, HA and EGFP.

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Insert

3XGal80-FLAG/HA/EGFP-Hsp70

Use

Tags

Expression

Mutation

Promoter

Available since

July 2, 2015

Availability

Academic Institutions and Nonprofits only

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pME DN-E-cadherin

Plasmid

#135209

Purpose

Dominate Negative zebrafish e-cadherin label

Depositor

David Tobin

Article

Cronan et al Immunity. 2016 Oct 18;45(4):861-876. doi

Insert

CDH1

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pMKO.1-shMki67

Plasmid

#160958

Purpose

Mki67 shRNA in pMKO.1 retroviral vector

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Mki67 (Mki67 Mouse)

Use

RNAi and Retroviral

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1 (-) + codon optimized Brn3a

Plasmid

#86829

Purpose

mammalian expression of the transcription factor Brn3a

Depositor

Carl Novina

Article

Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

Insert

Brn3a (POU4F1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 2, 2017

Availability

Academic Institutions and Nonprofits only

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pSK267

Plasmid

#129272

Purpose

"ZF only" yTRAP control to control for yTRAP reporter fluorescence changes not dependent on sensed protein

Depositor

Ahmad Khalil

Article

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Insert

NLS-VP16-ZF 43-8-NES

Use

Tags

6xHA

Expression

Yeast

Mutation

Promoter

SUP35

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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PGEX-6P1-hNF2-AR mutant

Plasmid

#107149

Purpose

bacterial expression

Depositor

Tina Izard

Article

Chinthalapudi et al Nat Commun. 2018 Apr 6;9(1):1338. doi: 1

Insert

neurofibromin 2 (NF2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

A585W-R588K

Promoter

Ptac

Available since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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pARF1-YFP

Plasmid

#11390

Depositor

Joel Swanson

Article

Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

Insert

ADP Ribosylation Factor 1 (ARF1 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only

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pGL2-50bp

Plasmid

#16295

Depositor

Wafik El-Deiry

Article

Wang et al Cancer Res. 2006 Jul 15. 66(14):6982-9.

Insert

p53 promoter fragment (TP53 Human)

Use

Luciferase

Tags

Expression

Mammalian

Mutation

fragment -38 to +12 relative to first transcripti…

Promoter

Available since

Dec. 14, 2007

Availability

Academic Institutions and Nonprofits only

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HA-Cdk9 T186A

Plasmid

#28103

Depositor

Andrew Rice

Article

Wang et al J Biol Chem. 2008 Nov 28. 283(48):33578-

Insert

Cdk9 (CDK9 Human)

Use

Tags

3x HA

Expression

Bacterial and Mammalian

Mutation

Contains T186A mutation in Cdk9

Promoter

Available since

March 15, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV-Nxph4-6A-HA

Plasmid

#128103

Purpose

Express HA tagged Nxph4-6A

Depositor

Huda Zoghbi

Article

Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

Insert

Nxph4 (Nxph4 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pETM33_PTK2_Focal-AT

Plasmid

#178485

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

PTK2_Focal-AT (PTK2 Human, Synthetic)

Use

Tags

His-GST

Expression

Bacterial

Mutation

Promoter

T7/LacO

Available since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR-Arl13b2

Plasmid

#40871

Depositor

Tamara Caspary

Article

Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

Insert

Arl13b (Arl13b Mouse)

Use

Gateway entry

Tags

Expression

Mutation

no stop codon

Promoter

Available since

Nov. 27, 2012

Availability

Academic Institutions and Nonprofits only
pGST-TEV-yVms1(1-417,delta(38-69))

Plasmid

#128739

Purpose

Bacterial overexpression and crystallization

Depositor

Chris Hill

Article

Nielson et al Mol Cell. 2017 Nov 16;68(4):673-685.e6.

Insert

Vms1 (VCP/Cdc48-associated mitochondria stress-responsive 1) (VMS1 Budding Yeast)

Use

Tags

GST

Expression

Bacterial

Mutation

residues 1-417 with deletion of 38-69

Promoter

T7

Available since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-Akt(K179M)

Plasmid

#39535

Depositor

Julian Downward

Article

Watton et al Curr Biol. 1999 Apr 22;9(8):433-6.

Insert

Akt(K179M) (Akt1 Mouse)

Use

Tags

6G linker, EGFP, and HA

Expression

Mammalian

Mutation

K179M

Promoter

Available since

Sept. 26, 2012

Availability

Academic Institutions and Nonprofits only