Ready-to-use AAV PHP.eB particles produced from AiP14496: pAAV-AiE0873m_3xC2-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN4496) (#214858). In addition to the viral particles, you will also receive purified AiP14496: pAAV-AiE0873m_3xC2-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN4496) plasmid DNA. minBG-driven expression of SYFP2 plus a separate nuclear FLAG epitope in striatal cholinergic neurons. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.

pET28a modified to contain TEV protease fusion tag instead of T7 tag

Used for expression of protein in E. coli as non-cleavable C-terminal 8His-tag fusions

C terminal dual protein A affinity tag with TEV cleavage site

N terminal dual protein A affinity tag with TEV cleavage site

C terminal MBP affinity tag with PreScission Protease cleavage site

N terminal MBP affinity tag with PreScission Protease cleavage site

Plasmids used in Integrated Genomics: A Discovery-Based Laboratory Course; please note that the C. elegans yeast two-hybrid cDNA library is unavailable

Used for expression of protein in E. coli as non-cleavable C-terminal Strep Tag fusions

Protein expression vector with a TEV cleavable N-terminal 6xHis-MBP and C-terminal StrepTagII

HO endonuclease expression

Protein expression vector with a TEV cleavable N-terminal 6xHis-MBP and C-terminal StrepTagII

Expression of RedStinger in insect

Used for expression of protein in E. coli as TEV cleavable N-terminal Avi-His8-tag and MBP fusions

Nuclear localised mRFP reporter construct with one half of split Venus