Addgene

We narrowed to 11,414 results for: 110

Showing: 7641 - 7660 of 11414 results

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Cherry-ICP0FxE-C3

Plasmid

#134562

Purpose

Expresses Cherry-ICP0 fusion protein with a RING finger deletion (FXE)

Depositor

Susan Janicki

Article

Newhart et al J Cell Sci. 2012 Nov 15;125(Pt 22):5489-

Insert

ICP0

Use

Tags

Cherry

Expression

Mammalian

Mutation

RING finger deletion (FXE)

Promoter

CMV

Available since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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Prab-3::NLS::GCaMP6s

Plasmid

#68119

Purpose

Nuclear Localized Fluorescent Calcium Indicator for pan-neuronal expression in C. Elegans

Depositor

Andrew Leifer

Article

Nguyen et al Proc Natl Acad Sci U S A. 2015 Dec 28. p

Insert

rab-3::NLS::GCaMP6s

Use

Tags

Expression

Worm

Mutation

Promoter

rab-3

Available since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pRRL_U2AF1_S34Y_mCherry

Plasmid

#84018

Purpose

expression of mutant U2AF1 in mammalian cells

Depositor

Robert Bradley

Article

Ilagan et al Genome Res. 2015 Jan;25(1):14-26. doi: 1

Insert

U2AF1 (U2AF1 Human)

Use

Lentiviral

Tags

FLAG and T2A-mCherry

Expression

Mammalian

Mutation

S34Y

Promoter

PGK

Available since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only
819 pGEX2T PTEN C124S

Plasmid

#10745

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

PTEN (PTEN Human)

Use

Tags

GST

Expression

Bacterial

Mutation

C124S

Promoter

Available since

Sept. 27, 2005

Availability

Academic Institutions and Nonprofits only
pKIBra-P2A-mCherry-Puro-sfGFP

Plasmid

#115686

Purpose

Knock-in of P2A-mCherry at the mouse Brachyury (T) gene

Depositor

David Suter

Article

Deluz et al Genes Dev. 2016 Nov 15;30(22):2538-2550.

Insert

P2A-mCherry-loxP-PGK-Puro-sfGFP-loxP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCVhyg-3xT7-CA-ChREBP

Plasmid

#171029

Purpose

Expresses 3xT7-tagged constitutive active form of ChREBP in mammalian cells

Depositor

Kai Ge

Article

Jang et al Genes Dev. 2021 Apr 22. pii: gad.347583.

Insert

CA-ChREBP (Mlxipl Mouse)

Use

Retroviral

Tags

3xT7

Expression

Mammalian

Mutation

amino acids 240 - 864

Promoter

5' LTR

Available since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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Dronpa-Lifeact-7

Plasmid

#54740

Purpose

Localization: Actin, Excitation: 503, Emission: 518

Depositor

Michael Davidson

Article

Rego et al Proc Natl Acad Sci U S A. 2012 Jan 17;10

Type

Empty backbone

Use

Tags

Dronpa-Lifeact

Expression

Mammalian

Mutation

Promoter

Available since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-JNKAR1

Plasmid

#61625

Purpose

FRET-based biosensor for monitoring JNK activity

Depositor

Jin Zhang

Article

Fosbrink et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

Insert

JNKAR1

Use

Tags

Citrine and ECFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 13, 2015

Availability

Academic Institutions and Nonprofits only

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mc2 blast

Plasmid

#206217

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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mc2 mNeon

Plasmid

#206218

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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811 pSG5L HA PTEN C124S

Plasmid

#10744

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

PTEN (PTEN Human)

Use

Tags

HA

Expression

Mammalian

Mutation

C124S

Promoter

Available since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

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pAAV-TetO(3G)-WPRE

Plasmid

#186206

Purpose

tTA dependent gene expression vector

Depositor

Naoshige Uchida

Article

Amo et al Nat Neurosci. 2022 Aug;25(8):1082-1092.

Type

Empty backbone

Use

AAV

Tags

Expression

Mutation

Promoter

TetO(3G)

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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p-dCas9-LSD1-K661A-Hygro

Plasmid

#104414

Purpose

transient expression of dCas9-dLSD1 fusion protein

Depositor

Stephan Beck

Insert

dLSD1 (KDM1A Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

IRATp970A0364D (Source Bioscience) has a P405H ch…

Promoter

CMV promoter

Available since

April 2, 2020

Availability

Academic Institutions and Nonprofits only

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SG3ΔENV CA M66I

Plasmid

#149687

Purpose

Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and M66I mutation in capsid (CA M66I).

Depositor

Wesley Sundquist

Article

Christensen et al Science. 2020 Oct 9;370(6513). pii: 370/

Insert

Capsid

Use

Lentiviral and Retroviral

Tags

Expression

Mammalian

Mutation

Changed Capsid residue Met 66 to Iso (CA M66I)

Promoter

Available since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pTRANS_253

Plasmid

#91125

Purpose

Transformation, Type: T-DNA, Plant Selection: PvUbi2:hpt II, Viral Replication: WDV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-beta-hRLuc-V5-6xbsMS2-MALAT_X

Plasmid

#147924

Purpose

Mammalian Expression of 6xbsMS2-Malat

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

6xbsMS2-Malat (Malat1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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Cas9-GFP_sg_mTfeb

Plasmid

#79006

Purpose

Expresses WT Cas9 and GFP, along with a sgRNA to mouse Tfeb.

Depositor

Reuben Shaw

Article

Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

Insert

sgTfeb (Tfeb Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 22, 2016

Availability

Academic Institutions and Nonprofits only

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PMXS-SLC2A3

Plasmid

#72877

Purpose

expresses SLC2A3 in mammalian cells

Depositor

David Sabatini

Article

Birsoy et al Nature. 2014 Apr 3;508(7494):108-12. doi

Insert

SLC2A3 (SLC2A3 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM3

Plasmid

#58389

Purpose

Expressed murine IFITM3 with an N-terminal HA tag in mammalian cells

Depositor

Jacob Yount, Howard Hang

Article

Yount et al Nat Chem Biol. 2010 Aug;6(8):610-4. doi:

Insert

IFITM3 (Ifitm3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV IE

Available since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only
TL024_lenti_hSyn:HaloTag-GluA1

Plasmid

#192517

Purpose

Expression of AMPA GluA1 with N terminal HaloTag fusion

Depositor

Adam Cohen

Article

Kim et al bioRxiv. 2023 May 29:2023.05.26.541296.

Insert

HaloTag-GluA1

Use

Lentiviral

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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