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Plasmid#143212PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertCTCFL (CTCFL Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC8A3_STOP
Plasmid#161352PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC8A3 (SLC8A3 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR221-SLC8A2_STOP
Plasmid#161340PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC8A2 (SLC8A2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
Rtn4a-GFP
Plasmid#61807PurposeHuman Rtn4a fluorescently tagged with GFP on the C-terminus for mammalian expressionDepositorInsertRtn4a (RTN4 Human)
UseTagsGFPExpressionMammalianMutationRtn4a Stop Codon removedPromoterCMVAvailable sinceFeb. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1 KBTBD4 WT
Plasmid#184624PurposeDoxycycline inducible lentiviral vector for N-terminal Flag tagged KBTBD4 WT expression in mammalian cellsDepositorInsertN-terminal Flag tagged KBTBD4 WT (KBTBD4 Human)
UseLentiviral; Doxycycline inducibleTagsExpressionMammalianMutationno mutationPromoterTRE promoter, Tet ONAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHAGE TRE dCas9-KRAB
Plasmid#50917PurposeTet-regulatable dCas9-KRAB 2nd generation lentiviral expression vectorDepositorInsertdCas9
UseCRISPR and LentiviralTags3xHA and KRABExpressionMammalianMutationD10A, H840A (catalytically inactive)PromoterTREAvailable sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-BsmBI_cassette-Nme/Nme2Cas9-sgRNA (KAC32)
Plasmid#133794PurposeU6 promoter sgRNA entry vector used for all NmeCas9 or Nme2Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Amrani et al. Genome Biology 2018DepositorInsertNmeCas9 and Nme2Cas9 sgRNA entry vector
UseTagsExpressionMammalianMutationsgRNA architecture from Amrani et al. Genome Biol…PromoterU6Available sinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCherry-BP1-2 pLPC-Puro
Plasmid#19835DepositorInsertFragment of p53-Binding Protein 1 (TP53BP1 Human)
UseRetroviralTagsmCherryExpressionMutationFragment of human 53BP1 aa 1220 - 1711PromoterAvailable sinceNov. 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGEM4Z-T7-5'UTR-mGreenLantern-3'UTR-A64
Plasmid#203349PurposeTemplate for T7 in vitro transcription of mGreenLantern mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tailDepositorInsertmGreenLantern
UseTemplate for t7-mediated in vitro transcription o…TagsFlanked by 5' and 3' UTRs from Xenopus …ExpressionMutationPromoterT7Available sinceJune 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKM200
Plasmid#13079DepositorInsertPtac-gam-bet-exo operon
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
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pANAP
Plasmid#48696Purposeallows co-translational incorporation of a fluorescent AA directly into the target protein; expresses amber suppressor tRNA and the synthetase AnapRSDepositorInserts8 copies of the amber suppressor tRNACUA-Leu
AnapRS
UseTagsExpressionMammalianMutationmutant E.coli leucyl synthetase specific for Anap…PromoterCMV and human H1 promoterAvailable sinceNov. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
INCENP
Plasmid#39185PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertINCENP (INCENP Human)
UseTagsGST-TevExpressionBacterialMutationComplex with AURKBA-c004PromoterAvailable sinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pet-15b-microID
Plasmid#172881Purposeexpresses microID in E. coliDepositorInsertmicroID
UseTagsmycExpressionBacterialMutationfragment ([aa2-171]) of aquifex aeolicus BirA wit…PromoterT7Available sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-D2s-L-Venus
Plasmid#19966DepositorInsertD2 short variant-monomeric Venus (DRD2 Aequorea Victoria, Human)
UseTagsFlag and monomeric VenusExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
YopH (residues 164-468)
Plasmid#79749PurposeThis plasmid encodes the active catalytic domain (amino acid residues 164-468) of YopH. Inteded for co-expression with human Tyr kinase plasmids to enhance bacterial kinase expression.DepositorInsertYopH (residues 164-468)
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLX307 P2A GFP PRDM14
Plasmid#193676PurposeConstitutive lentiviral expression of PRDM14DepositorInsertPRDM14 (PRDM14 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHSP02
Plasmid#117259PurposeGenome editing for Lactobacillus plantarum WCSF1DepositorInsertCas9, promotor P11-guide-RNA, homologous arms of Lp_0537
UseOtherTagsExpressionMutationPromoterAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pm-iATPSnFR1.1
Plasmid#102549Purposemembrane displayed ATP sensor for mammalian cellsDepositorInsertiATPSnFR1.1
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV:DsRed(FRT)GFP
Plasmid#31128DepositorInsertDsRed(FRT)GFP
UseFlp/frtTagsExpressionMammalianMutationPromoterAvailable sinceOct. 17, 2011AvailabilityAcademic Institutions and Nonprofits only