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Plasmid#66842PurposeExpresses two dimeric Broccoli aptamers in the F30 scaffold in mammalian cellsDepositorInsertTwo dimeric Broccoli aptamers in the F30 scaffold
UseTagsExpressionMammalianMutationPromoterU6Available sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV phSyn1(S)-tdTomato-WPRE (AAV5)
Viral Prep#51506-AAV5PurposeReady-to-use AAV5 particles produced from AAV phSyn1(S)-tdTomato-WPRE (#51506). In addition to the viral particles, you will also receive purified AAV phSyn1(S)-tdTomato-WPRE plasmid DNA. hSyn-driven tdTomato expression. These AAV preparations are suitable purity for injection into animals.DepositorPromoterphSyn1TagstdTomatoAvailable sinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
plRL2
Plasmid#163631PurposeOptimized M. tuberculosis CRISPRi plasmid; L5 integrating.DepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterTet-OnAvailable sinceAug. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
3xAP1pGL3 (3xAP-1 in pGL3-basic)
Plasmid#40342DepositorInsert3xAP-1
UseLuciferaseTagsLuciferaseExpressionMammalianMutationContains three canonical AP-1 binding sites (TGAC…PromoterAvailable sinceSept. 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
EBFP2-C1
Plasmid#54665PurposeLocalization: C1 Cloning Vector, Excitation: 383, Emission: 448DepositorTypeEmpty backboneUseTagsEBFP2ExpressionMammalianMutationPromoterAvailable sinceJuly 25, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLVX-EF1alpha-SARS-CoV-2-nsp10-2xStrep-IRES-Puro
Plasmid#141376PurposeLentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirusDepositorInsertSARS-CoV-2-nsp10 (ORF1ab SARS-CoV-2)
UseLentiviralTags2xStrepExpressionMammalianMutationhuman codon optimizedPromoterEF1alphaAvailable sinceApril 9, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAG vGlut1 mCherry
Plasmid#105010PurposeExcitatory presynaptic markerDepositorInsertSLC17A7
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
CS-TRE-BMI1-Ubc-rtTA
Plasmid#198191PurposeGeneration of Expandable megakaryocyte cell lines from human iPS cellsDepositorInserthBMI1 (BMI1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-R1R2_EF1aVP64dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA
Plasmid#113341PurposeExpresses VP64-dCas9-VP64 and MS2-p65-HSF1 fusion proteinsDepositorInsertVP64-dCas9-VP64-T2A-MS2-p65-HSF1
UsePiggybacTagsExpressionMammalianMutationPromoterAvailable sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-HLA-Flag-natT1R3
Plasmid#113950Purposemammalian expression plasmid for FLAG-tagged human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chainDepositorInsertT1R3 (TAS1R3 Human)
UseTagsFLAG and Signal/leader sequence from HLA class I …ExpressionMammalianMutationresidues 21-852PromoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCONR221
Plasmid#216146PurposeGateway Donor Vector with attP1 and attP2 sites modified from PDONR221 and Ampicillin resistanceDepositorTypeEmpty backboneUseCloning backboneTagsExpressionMutationPromoterAvailable sinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAcGFP-stop-IVT
Plasmid#205146PurposeIVT template of AcGFP stop codon between the ORF and MCS and polyADepositorInsertAcGFP1
UseIn-vitro transcriptionTagsExpressionMutationPromoterT7Available sinceAug. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCE-hOCT3/4
Plasmid#41813PurposeNon-integrating (episomal) expression of human OCT3/4DepositorInsertPOU5F1 (POU5F1 Human)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceApril 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pILGFP91
Plasmid#83548PurposeUsed to evaluate the expression output of PGK1 promoter with yEGFP used as the reporter gene.DepositorInsertPGK1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterPGK1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLentiPGK DEST H2B-iRFP670
Plasmid#90237PurposeLentiviral vector to express H2B-iRFP670 under PGK promoterDepositorInsertH2B
UseLentiviralTagsiRFP670ExpressionMammalianMutationPromoterPGKAvailable sinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Kir2.1-T2A-tdTomato
Plasmid#60598PurposeExpresses Kir2.1-T2A-tdTomatoDepositorInsertKir2.1-T2A-tdTomato (Kcnj2 Mouse)
UseTagsmycExpressionMammalianMutationPromoterAvailable sinceNov. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
Lenti-human-UBDUB-gRNA-puromycin-IRES-CFP
Pooled Library#171531PurposePooled gRNA library against human ubiquitination and de-ubiquitination complex genes (UBDUB)DepositorExpressionMammalianSpeciesHomo sapiensUseLentiviralAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
v3em-Cterm-PE2max-∆RNaseH-dualU6
Plasmid#198735PurposeAAV genome encoding C-terminal PE2max and U6 expression cassettesDepositorInsertNpuC-CtermPE2max∆RNaseH
UseAAVTagsExpressionMutationPromoterCbhAvailable sinceMay 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV4-ApoE3
Plasmid#87086PurposeExpresses ApoE3 in Mammalian cellsDepositorInsertApolipoprotein E3 (APOE Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Vinculin-23
Plasmid#55159PurposeLocalization: Focal Adhesions, Excitation: 587, Emission: 610DepositorInsertVinculin (VCL Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only