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Plasmid#208275PurposeExpresses Fus-BcLOV-SOS for optogenetic activation of Ras/Erk signaling. Includes an mCherry tag.DepositorInsertFus-BcLOV-mCh-SOS
UseLentiviralTagsExpressionMutationPromoterSFFVAvailable sinceJan. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
p201N Cas9
Plasmid#59175PurposeCas9 driven by double 35S, nptII for plant selection, I-PpoI site to accept gRNA from pUC gRNA ShuttleDepositorInsertsCas9
nptII
UseCRISPRTagsExpressionMutationPromoter2x35S and StUbi-3PAvailable sinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSGEMKv10.1
Plasmid#85704PurposeXenopus laevis oocyte expression of Kv10.1DepositorInsertKCNH1 (KCNH1 Human)
UseXenopus expressionTagsExpressionMutationPromoterAvailable sinceDec. 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEvolvR-enCas9-PolI3M-TBD
Plasmid#113077PurposeExpresses enCas9 fused to PolI3M-TBD in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.DepositorInsertenCas9-PolI3M-TBD
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
YSEHPTFTSQY_epitope
Plasmid#165016PurposeExpression of CMV UL83 (363-373) fused to IL-2 signal sequenceDepositorInsertCMV UL83 (363-373)
UseLentiviralTagshuman IL2 signal sequence at N-termExpressionMammalianMutationPromoterCMVAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
His6-MBP-uTEV3
Plasmid#135464PurposeBacterial expression of uTEV3 (full-length) proteaseDepositorInsertHis6-MBP-uTEV3
UseTagsMBP, His6ExpressionBacterialMutationS219V mutation (improves stability) , I138T/S153N…PromoterT5Available sinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
p201H Cas9
Plasmid#59176PurposeCas9 driven by double 35S, hygromycin resistance for plant selection, I-PpoI site to accept gRNA from pUC gRNA ShuttleDepositorInsertsCas9
hph
UseCRISPRTagsExpressionMutationPromoter2x35S and StUbi-3PAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMpGE014
Plasmid#108682PurposeAll-in-one genome editing vector in M. polymorpha (chlorsulfuron resistant)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
CFP-FKBP (CF)
Plasmid#20160DepositorInsertCFP-FKBP (FKBP1A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDV17_pCMV-MCP-ADAR2dd(E488Q)-NES
Plasmid#200406PurposeExpresses a compact, engineered version of ADAR2 constitutively in mammalian cellsDepositorInsertMCP-ADAR2dd(E488Q)-NES
UseSynthetic BiologyTagsExpressionMammalianMutationE488Q (hyperactive)PromoterCMVAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
p11d-sctRPA
Plasmid#102614PurposeExpression of S. cerevisiea RPA in E.coliDepositorUseTagsExpressionBacterialMutationRFA2 has intron deletedPromoterT7Available sinceOct. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-H2A-TORCAR
Plasmid#175016PurposeTORCAR targeted to the nucleus via histone H2A.DepositorInsertH2A-TORCAR
UseTagsHistone H2AExpressionMammalianMutationPromoterCMVAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMT525
Plasmid#131392PurposeL1RP retrotransposition reporterDepositorInsertL1RP GFP-AI
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-loxSTOPlox-LynEGFP
Plasmid#133922PurposeReporter expressing membrane targeted EGFP for visualization of neuronal morphology upon Cre-based recombinationDepositorInsertLynEGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
mTurquoise2-3xrGBD
Plasmid#176100PurposeVisualization of active RhoDepositorInsertrhotekin (Rtkn Mouse)
UseTagsExpressionMammalianMutationPromoterCMVdelAvailable sinceOct. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
N-WASP(CRIB)-mScarlet-I-N-WASP(CRIB)
Plasmid#191451PurposeVisualizing endogenous & active Cdc42DepositorInsertN-WASP
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
CAG FUCCI
Plasmid#175266PurposeBicistronic FUCCI construct driven by a CAG promoter. Contains I-SceI sites for transgenesisDepositorInsertFUCCI
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-U6-sgRNA-PGK-puromycin
Plasmid#51133Purposeexpresses sgRNA from the U6 promoter.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
WASp(CRIB)-HaloTag-WASp(CRIB)
Plasmid#191452PurposeVisualizing endogenous & active Cdc42DepositorInsertWASp
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSb_init containing the MBP sybody Sb_MBP#1
Plasmid#132699Purposeexpresses the MBP sybody Sb_MBP#1 in E.coli, to use as positive control in ELISADepositorInsertsybody_MBP#1
UseTagsExpressionBacterialMutationPromoterpBADAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only