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Showing: 7601 - 7620 of 21689 results

  5. pOCC5

    Plasmid
    #118863
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C protease
    Depositor
    PEPC MPI CBG, Marc Bickle
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-3C-NotI-ccdB-AscI-stop-HindIII cassette
    Use
    Tags
    HIS6, cleavable with 3C protease
    Expression
    Insect
    Mutation
    Promoter
    polH
    Available since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118863-plasmid-map-sequence-id-232276

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  11. pTG131

    Plasmid
    #198640
    Purpose
    Thermosensitive pSC101-based plasmid expressing a sgRNA under the control of the J23119 promoter and Cas9 under the control of the DAPG-inducible PhlF promoter
    Depositor
    David Bikard
    Article
    Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.
    Insert
    Cas9
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pPhlF
    Available since
    June 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    198640-plasmid-map-sequence-id-396878

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  13. Stx1a-12aa-mCer

    Plasmid
    #53236
    Purpose
    Fluorescent probe: Syntaxin 1A, C-terminally-tagged with monomeric Cerulean, connected with a 12 amino acid linker. Expressed in mammalian cells, used with SNAP25B-12aa-mCit to measure FRET & dispers.
    Depositor
    Robert Zucker
    Article
    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.
    Insert
    Syntaxin 1A (Stx1a Rat)
    Use
    Tags
    12aa-linker and mCerulean
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 9, 2014
    Availability
    Academic Institutions and Nonprofits only

  16. pcDNA-LBR-EGFP-polyA

    Plasmid
    #59748
    Purpose
    Express LBR-EGFP in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes. This LBR contains N-terminal domain only.
    Depositor
    Yi Zhang
    Article
    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-
    Insert
    LBR-EGFP
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and T7
    Available since
    Sept. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    59748-plasmid-map-sequence-id-94052

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  18. BPK2139

    Plasmid
    #65776
    Purpose
    Human expression vector for S aureus Cas9: CAG-humanSaCas9-NLS-3xFLAG
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Staphylococcus aureus Cas9-NLS-3xFlag
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65776-plasmid-map-sequence-id-112651

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Showing: 7601 - 7620 of 21689 results