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Plasmid#55151PurposeLocalization: Actin/Focal Adhesions, Excitation: 587, Emission: 610DepositorInsertVASP
UseTagsmCherryExpressionMammalianMutationA209T in VASPPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET21a-AspRS-His
Plasmid#124106PurposeBacterial expression plasmid for PURE system componentsDepositorInsertAspartate-tRNA ligase (aspS Escherichia coli)
UseTags6x-HisExpressionBacterialMutationPromoterAvailable sinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn1-DIO-Opto-cytTrkB(E281A)-HA
Plasmid#180590PurposeOpto-cytTrkBDepositorInsertTrkB
UseAAVTagsExpressionMutationPHR(E281A)PromoterAvailable sinceFeb. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
hCRISPRi_dual_1_2
Pooled Library#187246PurposeUltra-compact, human genome-wide CRISPRi library targeting each gene with a single element encoding a dual sgRNA cassette.DepositorSpeciesSyntheticUseLentiviralAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pYPQ230 (LbCpf1)
Plasmid#86210PurposeLbCpf1 Gateway entry plasmidDepositorInsertLbCpf1
UseCRISPR; Gateway compatible lbcpf1 entry cloneTags5' and 3' NLSExpressionPlantMutationPromoterAvailable sinceApril 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CMV
Plasmid#72265PurposeExpress gene of interest under CMV promoterDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV.CMV.ffLuciferase.SV40
Plasmid#105532PurposeAAV expression of firefly luciferase from CMV promoterDepositorHas ServiceAAV8InsertLuciferase
UseAAV and LuciferaseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-Cas9-NLS-6xHis
Plasmid#62933PurposeExpression of Cas9-NLS-6xHis in bacterial cellsDepositorInsertCas9-NLS-6xHis
UseTags6xHis tag and NLSExpressionBacterialMutationPromoterT7Available sinceMay 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
mTB3-BC2 nanobody
Plasmid#210208PurposeType 3 phage-display vector that contains kanamycin resistance and displays the BC2 nanobody at the N-terminus of mature protein III.DepositorInsertBC2 Nanobody
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF1a_HNF1A_P2A_Hygro_Barcode
Plasmid#120448PurposeBarcoded lentiviral vector to express HNF1A in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertHNF1A (HNF1A Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
PyronicSF-mRuby2/pBI-CMV1
Plasmid#124830PurposeHighly resposive GFP-based pyruvate nanosensor.DepositorInsertsPyronicSF
mRuby2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
PTGER3-Tango
Plasmid#66485PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertEP3-I (PTGER3 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDD426
Plasmid#202079PurposeFrame +1/-0 selector for NHEJ knock-inDepositorInsertpEF1a-mCherry-Cas9 + Frame +1/-0 sgRNA
UseCRISPRTagsExpressionMutationPromoterEF1aAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
eGFP STAT1 WT
Plasmid#12301DepositorInsertSTAT1 (STAT1 Human)
UseTagseGFPExpressionMammalianMutationPromoterAvailable sinceSept. 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
dCas9-10xSunTag(22aa)
Plasmid#215745PurposeExpresses dCas9 with 10 GCN4 epitopes separated by 22 amino acid linkersDepositorInsertdCas9-10xSunTag
UseTagsExpressionMammalianMutationPromoterchicken beta-actin promoterAvailable sinceApril 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-TRE-dCas9-KRAB-MeCP2
Plasmid#122267PurposeSP-dCas9-KRAB-MeCP2 with doxycycline-inducible expressionDepositorInsertdCas9-KRAB-MeCP2
UseTagsdCas9-KRAB-MeCP2ExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRRL_U2AF1_S34F_mCherry
Plasmid#84019Purposeexpression of mutant U2AF1 in mammalian cellsDepositorInsertU2AF1 (U2AF1 Human)
UseLentiviralTagsFLAG and T2A-mCherryExpressionMammalianMutationS34FPromoterPGKAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
PUMA Frag1-Luc
Plasmid#16591DepositorInsertPUMA promotor fragment (BBC3 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceApril 7, 2008AvailabilityAcademic Institutions and Nonprofits only -
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pET-pA-Hia5
Plasmid#174372PurposeSEE DEPOSITOR COMMENTS BELOW. Plasmid for IPTG-inducible expression and purification of protein A-Hia5-6xHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)DepositorInsertprotein A - hia5
UseTags6xHis and Protein AExpressionBacterialMutationPromoterAvailable sinceSept. 6, 2021AvailabilityAcademic Institutions and Nonprofits only